In vivo application of poly-3-hydroxyoctanoate as peripheral nerve graft

Objective: This study aims to investigate the degree of biocompatibility and neuroregeneration of a polymer tube, poly-3-hydroxyoctanoate (PHO) in nerve gap repair. Methods: Forty Wistar Albino male rats were randomized into two groups: autologous nerve gap repair group and PHO tube repair group. In each group, a 10-mm right sciatic nerve defect was created and reconstructed accordingly. Neuroregeneration was studied by sciatic function index (SFI), electromyography, and immunohistochemical studies on Days 7, 21, 45 and 60 of implantation. Biocompatibility was analyzed by the capsule formation around the conduit. Biodegradation was analyzed by the molecular weight loss in vivo. Results: Electrophysiological and histomorphometric assessments demonstrated neuroregeneration in both groups over time. In the experimental group, a straight alignment of the Schwann cells parallel to the axons was detected. However, autologous nerve graft seems to have a superior neuroregeneration compared to PHO grafts. Minor biodegradation was observed in PHO conduit at the end of 60 d. Conclusions: Although neuroregeneration is detected in PHO grafts with minor degradation in 60 d, autologous nerve graft is found to be superior in axonal regeneration compared to PHO nerve tube grafts. PHO conduits were found to create minor inflammatory reaction in vivo, resulting in good soft tissue response. © 2013 Zhejiang University and Springer-Verlag Berlin Heidelberg.

Dergi Adı Journal of Zhejiang University: Science B
Dergi Cilt Bilgisi 14
Dergi Sayısı 11
Sayfalar 993 - 1003
Yayın Yılı 2013
Eser Adı
[dc.title]
In vivo application of poly-3-hydroxyoctanoate as peripheral nerve graft
Yazar
[dc.contributor.author]
Hazer, Derya Burcu
Yazar
[dc.contributor.author]
Bal, Ercan
Yazar
[dc.contributor.author]
Nurlu, Gülay
Yazar
[dc.contributor.author]
Benli, Kemal
Yazar
[dc.contributor.author]
Balcı, Serdar
Yazar
[dc.contributor.author]
Öztürk, Feral
Yazar
[dc.contributor.author]
Hazer, Baki
Yayın Yılı
[dc.date.issued]
2013
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Objective: This study aims to investigate the degree of biocompatibility and neuroregeneration of a polymer tube, poly-3-hydroxyoctanoate (PHO) in nerve gap repair. Methods: Forty Wistar Albino male rats were randomized into two groups: autologous nerve gap repair group and PHO tube repair group. In each group, a 10-mm right sciatic nerve defect was created and reconstructed accordingly. Neuroregeneration was studied by sciatic function index (SFI), electromyography, and immunohistochemical studies on Days 7, 21, 45 and 60 of implantation. Biocompatibility was analyzed by the capsule formation around the conduit. Biodegradation was analyzed by the molecular weight loss in vivo. Results: Electrophysiological and histomorphometric assessments demonstrated neuroregeneration in both groups over time. In the experimental group, a straight alignment of the Schwann cells parallel to the axons was detected. However, autologous nerve graft seems to have a superior neuroregeneration compared to PHO grafts. Minor biodegradation was observed in PHO conduit at the end of 60 d. Conclusions: Although neuroregeneration is detected in PHO grafts with minor degradation in 60 d, autologous nerve graft is found to be superior in axonal regeneration compared to PHO nerve tube grafts. PHO conduits were found to create minor inflammatory reaction in vivo, resulting in good soft tissue response. © 2013 Zhejiang University and Springer-Verlag Berlin Heidelberg.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Axonal spurting
Konu Başlıkları
[dc.subject]
Biodegradable polymer
Konu Başlıkları
[dc.subject]
Nerve grafting
Konu Başlıkları
[dc.subject]
Neuroregeneration
Künye
[dc.identifier.citation]
Hazer, D. B., Bal, E., Nurlu, G., Benli, K., Balci, S., Öztürk, F. ve Hazer, B. (2013). In vivo application of poly-3-hydroxyoctanoate as peripheral nerve graft. Journal of Zhejiang University SCIENCE B, 14(11), 993-1003. doi:10.1631/jzus.B1300016
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1673-1581
Sponsor YAYINCI
[dc.description.sponsorship]
Ministry of Health of the People's Republic of China
Sponsor YAYINCI
[dc.description.sponsorship]
(2Atatürk Research and Medical Center, Neurosurgery Clinic, Ministry of Health of the Republic of Turkey, Ankara 06110, Turkey) (3Department of Neurology, Faculty of Medicine, School of Medicine, Hacettepe University, Ankara 06100, Turkey) (4Department of Neurosurgery, Faculty of Medicine, School of Medicine, Hacettepe University, Ankara 06100, Turkey) (5Atatürk Research and Medical Center, Department of Pathology, Yıldırım Beyazıt University, Ankara 06110, Turkey) (6Department of Histology and Embryology, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla 48000, Turkey) (7Department of Chemistry, Bülent Ecevit University, Zonguldak 67100, Turkey) †E-mail: burcuhazer@hotmail.com Received Jan. 16, 2013; Revision accepted May 28, 2013; Crosschecked Oct. 21, 2013
İlk Sayfa Sayısı
[dc.identifier.startpage]
993
Son Sayfa Sayısı
[dc.identifier.endpage]
1003
Dergi Adı
[dc.relation.journal]
Journal of Zhejiang University: Science B
Dergi Sayısı
[dc.identifier.issue]
11
Dergi Cilt Bilgisi
[dc.identifier.volume]
14
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1631/jzus.B1300016
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/6197
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neuroregeneration autologous repair grafts superior compared sciatic groups conduit analyzed detected parallel However Schwann alignment straight experimental biodegradation observed Objective Heidelberg Berlin Springer-Verlag University Zhejiang response tissue resulting Conclusions reaction inflammatory create conduits regeneration axonal
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