Antimicrobial effect of polymer-based silver nanoparticle coated pedicle screws: Experimental research on biofilm inhibition in Rabbits

Study Design. Antimicrobial effect of a novel silverimpregnated pedicle screw in rabbits. Objective. A novel spine implant model was designed to study the antimicrobial effect of a modified Titanium (Ti) pedicle screws with methicillin-resistant Staphylococcus aureus (MRSA) in multiple surgical sites in the lumbar spine of a rabbit. Summary of Background Data. Infection in spinal implant is of great concern. Anti-infection strategies must be tested in relevant animal models that will lead to appropriate clinical studies. Methods. Fourteen New Zealand white rabbits were divided into 2 groups: group 1: infected unmodified Ti screw group (n=6), and group 2: infected polyethylene glycol grafted, polypropylene-based silver nanoparticle (PP-g-PEG-Ag) covered Ti screw group (n=6), and 2 rabbits as sterile (sham-operated and control) group. In all groups, left L4-right L6 vertebra levels were exposed and screws were drilled to transverse processes after contamination of burr holes and surrounding tissue with 0.1mL of 106 colony forming units (CFU) MRSA solutions in groups 1 and 2. After 21 days, samples were collected and infection was analyzed via light and scanning electron microscopy and culturing. Silver nanoparticles (Ag-NP) on the screws and tissues were assayed pre and postoperatively. Results. The bacterial colony count for modified-Ti screw group was lower than for unmodified Ti screw (17.2 versus 200 x 103 CFU/mL, P=0.029) with less biofilm formation. There was no difference in duration of surgery among groups and within the surgical sites. Ag-NPs were detected on the screw surface postoperatively. Conclusion. This novel experimental design of implantation in rabbits is easy to apply and resembles human stabilization technique. Modified Ti screws were shown to have antimicrobial effect especially inhibiting the biofilm formation. This anchored Ag NPs that remained after 21st day of implantation shows that it is resistant to tapping forces of the screw. © 2016 Wolters Kluwer Health, Inc. All rights reserved.

Dergi Adı Spine
Dergi Cilt Bilgisi 41
Dergi Sayısı 6
Sayfalar E323 - E329
Yayın Yılı 2016
Eser Adı
[dc.title]
Antimicrobial effect of polymer-based silver nanoparticle coated pedicle screws: Experimental research on biofilm inhibition in Rabbits
Yazar
[dc.contributor.author]
Hazer, Derya Burcu
Yazar
[dc.contributor.author]
Sakar, Mustafa
Yazar
[dc.contributor.author]
Dere, Yelda
Yazar
[dc.contributor.author]
Altınkanat, Gülşen
Yazar
[dc.contributor.author]
Ziyal, M. İbrahim
Yazar
[dc.contributor.author]
Hazer, Baki
Yayın Yılı
[dc.date.issued]
2016
Yayıncı
[dc.publisher]
Lippincott Williams and Wilkins
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Study Design. Antimicrobial effect of a novel silverimpregnated pedicle screw in rabbits. Objective. A novel spine implant model was designed to study the antimicrobial effect of a modified Titanium (Ti) pedicle screws with methicillin-resistant Staphylococcus aureus (MRSA) in multiple surgical sites in the lumbar spine of a rabbit. Summary of Background Data. Infection in spinal implant is of great concern. Anti-infection strategies must be tested in relevant animal models that will lead to appropriate clinical studies. Methods. Fourteen New Zealand white rabbits were divided into 2 groups: group 1: infected unmodified Ti screw group (n=6), and group 2: infected polyethylene glycol grafted, polypropylene-based silver nanoparticle (PP-g-PEG-Ag) covered Ti screw group (n=6), and 2 rabbits as sterile (sham-operated and control) group. In all groups, left L4-right L6 vertebra levels were exposed and screws were drilled to transverse processes after contamination of burr holes and surrounding tissue with 0.1mL of 106 colony forming units (CFU) MRSA solutions in groups 1 and 2. After 21 days, samples were collected and infection was analyzed via light and scanning electron microscopy and culturing. Silver nanoparticles (Ag-NP) on the screws and tissues were assayed pre and postoperatively. Results. The bacterial colony count for modified-Ti screw group was lower than for unmodified Ti screw (17.2 versus 200 x 103 CFU/mL, P=0.029) with less biofilm formation. There was no difference in duration of surgery among groups and within the surgical sites. Ag-NPs were detected on the screw surface postoperatively. Conclusion. This novel experimental design of implantation in rabbits is easy to apply and resembles human stabilization technique. Modified Ti screws were shown to have antimicrobial effect especially inhibiting the biofilm formation. This anchored Ag NPs that remained after 21st day of implantation shows that it is resistant to tapping forces of the screw. © 2016 Wolters Kluwer Health, Inc. All rights reserved.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Ag nanoparticles
Konu Başlıkları
[dc.subject]
Antimicrobial
Konu Başlıkları
[dc.subject]
Biofilm
Konu Başlıkları
[dc.subject]
Infection
Konu Başlıkları
[dc.subject]
Polyethylene glycol
Konu Başlıkları
[dc.subject]
Polypropylene
Konu Başlıkları
[dc.subject]
Rabbit
Konu Başlıkları
[dc.subject]
Spine
Konu Başlıkları
[dc.subject]
Spine implant model
Künye
[dc.identifier.citation]
Hazer, D. B., Sakar, M., Dere, Y., Altinkanat, G., Ibrahim Ziyal, M. ve Hazer, B. (2016). Antimicrobial effect of polymer-based silver nanoparticle coated pedicle screws: Experimental research on biofilm inhibition in Rabbits. Spine, 41(6), E323–E329. doi:10.1097/BRS.0000000000001223
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0362-2436
İlk Sayfa Sayısı
[dc.identifier.startpage]
E323
Son Sayfa Sayısı
[dc.identifier.endpage]
E329
Dergi Adı
[dc.relation.journal]
Spine
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
41
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1097/BRS.0000000000001223
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4310
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
48
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 19:11
Google Kontrol
Tıklayınız
screws rabbits groups effect postoperatively unmodified infected implantation biofilm formation surgical implant antimicrobial pedicle colony electron assayed scanning CFU/mL analyzed infection culturing microscopy Silver (Ag-NP) Results bacterial nanoparticles modified-Ti tissues versus Conclusion difference forces remained
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms