Protective effects of L-arginine on rat terminal ileum subjected to ischemia/reperfusion

OBJECTIVES: Studies have shown that nitric oxide (NO) may play a major role in sustaining mucosal integrity; however, NO has been also implicated in the pathogenesis of ischemia/reperfusion (I/R)-related tissue injury. We investigated the effects of L-arginine and N-nitro L-arginine methyl ester (L-NAME) on the acetylcholine-induced contractile response of ileum and the levels of malondialdehyde (MDA) and reduced glutathione (GSH). Histopathological changes were also evaluated in ileal preparations. MATERIALS AND METHODS: Male Wistar Albino rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (3 hours). Four groups were designed: sham-operated control; I/R; I/R and L-arginine pretreatment; and I/R and L-NAME pretreatment. After reperfusion, ileum specimens were collected to determine the parameters mentioned above. RESULTS: Following reperfusion, a significant decrease in acetylcholine-induced contractile response, an increase in lipid peroxidation, a decrease in GSH content, and mucosal damage of the ileal preparations were observed. We showed that decreased contractility, increased lipid peroxidation, and reduced GSH content have been reversed by L-arginine but not by L-NAME. Mucosal injury was significantly lowered in the L-arginine group. CONCLUSIONS: Treatment with L-arginine exerted a protective effect in intestinal I/R injury, which was mediated in part by regulating MDA and GSH levels, consequently ameliorating impaired contractile response and mucosal injury. © 2008 Lippincott Williams & Wilkins, Inc.

Dergi Adı Journal of Pediatric Gastroenterology and Nutrition
Dergi Cilt Bilgisi 46
Dergi Sayısı 1
Sayfalar 29 - 35
Yayın Yılı 2008
Eser Adı
[dc.title]
Protective effects of L-arginine on rat terminal ileum subjected to ischemia/reperfusion
Yazar
[dc.contributor.author]
Sayan, Hale
Yazar
[dc.contributor.author]
Özaçmak, Veysel Haktan
Yazar
[dc.contributor.author]
Altaner, Şemsi
Yazar
[dc.contributor.author]
Aktaş, R. Gülhan
Yazar
[dc.contributor.author]
Arslan, S. Oktay
Yayın Yılı
[dc.date.issued]
2008
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
OBJECTIVES: Studies have shown that nitric oxide (NO) may play a major role in sustaining mucosal integrity; however, NO has been also implicated in the pathogenesis of ischemia/reperfusion (I/R)-related tissue injury. We investigated the effects of L-arginine and N-nitro L-arginine methyl ester (L-NAME) on the acetylcholine-induced contractile response of ileum and the levels of malondialdehyde (MDA) and reduced glutathione (GSH). Histopathological changes were also evaluated in ileal preparations. MATERIALS AND METHODS: Male Wistar Albino rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (3 hours). Four groups were designed: sham-operated control; I/R; I/R and L-arginine pretreatment; and I/R and L-NAME pretreatment. After reperfusion, ileum specimens were collected to determine the parameters mentioned above. RESULTS: Following reperfusion, a significant decrease in acetylcholine-induced contractile response, an increase in lipid peroxidation, a decrease in GSH content, and mucosal damage of the ileal preparations were observed. We showed that decreased contractility, increased lipid peroxidation, and reduced GSH content have been reversed by L-arginine but not by L-NAME. Mucosal injury was significantly lowered in the L-arginine group. CONCLUSIONS: Treatment with L-arginine exerted a protective effect in intestinal I/R injury, which was mediated in part by regulating MDA and GSH levels, consequently ameliorating impaired contractile response and mucosal injury. © 2008 Lippincott Williams & Wilkins, Inc.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Contractile activity
Konu Başlıkları
[dc.subject]
Ileum
Konu Başlıkları
[dc.subject]
Ischemia and reperfusion
Konu Başlıkları
[dc.subject]
Nitric oxide
Künye
[dc.identifier.citation]
Sayan, H., Ozacmak, V. H., Altaner, S., Aktas, R. G., & Arslan, S. O. (2008). Protective Effects of L-Arginine on Rat Terminal Ileum Subjected to Ischemia/Reperfusion. Journal of Pediatric Gastroenterology and Nutrition, 46(1). https://journals.lww.com/jpgn/Fulltext/2008/01000/Protective_Effects_of_L_Arginine_on_Rat_Terminal.7.aspx
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0277-2116
İlk Sayfa Sayısı
[dc.identifier.startpage]
29
Son Sayfa Sayısı
[dc.identifier.endpage]
35
Dergi Adı
[dc.relation.journal]
Journal of Pediatric Gastroenterology and Nutrition
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
46
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1097/01.mpg.0000304450.54057.96
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/7236
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L-arginine injury contractile mucosal reperfusion response pretreatment L-NAME levels reduced preparations content peroxidation decrease acetylcholine-induced exerted significant impaired Lippincott increase Williams Wilkins Following observed RESULTS mentioned parameters determine collected specimens damage ameliorating showed mediated Treatment
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