Electrochemical determination of urea using a gold nanoparticle-copolymer coated-enzyme modified gold electrode

A new amperometric urea biosensor based on gold nanoparticle embedded poly(propylene-co-imidazole) was developed for the determination of urea. The urease adsorbed on the polymeric film catalyzed the hydrolysis of urea to ammonium and bicarbonate ions and the ammonium was then electrooxidized on the gold electrode with the aid of gold nanoparticles at +0.2 V versus Ag/AgCl using differential pulse voltammetry. The biosensor provided a linear current response to urea concentration from 0.1 to 30 mM, a detection limit of 36 µM, a relative standard deviation of 2.43% (n = 18), and excellent storage stability, as the current decrease was only 3% after 75 days. The operation of the biosensor was evaluated by the analysis of municipal sewage wastewater collected from the inlet pipe of the treatment plant of Zonguldak City in Turkey. The effects of possible interferants were also characterized. © 2018, © 2018 Taylor & Francis.

Eser Adı
[dc.title]
Electrochemical determination of urea using a gold nanoparticle-copolymer coated-enzyme modified gold electrode
Yazar
[dc.contributor.author]
Korkut, Şeyda
Yazar
[dc.contributor.author]
Uzunçar, Sinan
Yazar
[dc.contributor.author]
Kılıç, Muhammet Samet
Yazar
[dc.contributor.author]
Hazer, Baki
Yayın Yılı
[dc.date.issued]
2019
Yayıncı
[dc.publisher]
Taylor and Francis Inc.
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
A new amperometric urea biosensor based on gold nanoparticle embedded poly(propylene-co-imidazole) was developed for the determination of urea. The urease adsorbed on the polymeric film catalyzed the hydrolysis of urea to ammonium and bicarbonate ions and the ammonium was then electrooxidized on the gold electrode with the aid of gold nanoparticles at +0.2 V versus Ag/AgCl using differential pulse voltammetry. The biosensor provided a linear current response to urea concentration from 0.1 to 30 mM, a detection limit of 36 µM, a relative standard deviation of 2.43% (n = 18), and excellent storage stability, as the current decrease was only 3% after 75 days. The operation of the biosensor was evaluated by the analysis of municipal sewage wastewater collected from the inlet pipe of the treatment plant of Zonguldak City in Turkey. The effects of possible interferants were also characterized. © 2018, © 2018 Taylor & Francis.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Differential pulse voltammetry
Konu Başlıkları
[dc.subject]
gold nanoparticles
Konu Başlıkları
[dc.subject]
poly(propylene-co-imidazole)
Konu Başlıkları
[dc.subject]
sewage wastewater
Konu Başlıkları
[dc.subject]
Urea
Künye
[dc.identifier.citation]
Korkut, S., Uzuncar, S., Kilic, M. S. ve Hazer, B. (2019). Electrochemical determination of urea using a gold nanoparticle-copolymer coated-enzyme modified gold electrode. Instrumentation Science & Technology, 47(1), 1-18. doi:10.1080/10739149.2018.1447486
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1073-9149
Sponsor YAYINCI
[dc.description.sponsorship]
BEU-2016-77047330-01
Sponsor YAYINCI
[dc.description.sponsorship]
This work was supported by the Bulent Ecevit University Research Fund under Grant [number BEU-2016-77047330-01].
İlk Sayfa Sayısı
[dc.identifier.startpage]
1
Son Sayfa Sayısı
[dc.identifier.endpage]
18
Dergi Adı
[dc.relation.journal]
Instrumentation Science and Technology
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
47
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1080/10739149.2018.1447486
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5586
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09 Şubat 2024 06:51
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biosensor current ammonium operation evaluated decrease stability storage excellent treatment analysis Turkey Francis Taylor characterized interferants possible effects municipal Zonguldak standard collected wastewater sewage deviation relative polymeric electrode electrooxidized bicarbonate hydrolysis catalyzed adsorbed urease determination
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