Electrochemical, continuous-flow determination of p-benzoquinone on a gold nanoparticles poly(propylene-co-imidazole) modified gold electrode

A novel continuous flow biosensor based on gold nanoparticles and poly(propylene-co-imidazole) was developed for the online determination of p-benzoquinone. The amperometric response was measured as a function of p-benzoquinone concentration at an applied potential of -50 mV. The hydrogen peroxide concentration was optimized and fixed at 1 mM in samples. The mass transfer resistance of the copolymer film was minimized, and the flow cell was regenerated quickly at 1 mL/min. The resulting device provided good analytical performance based on a linear dynamic range from 5–100 µM, a short response time of 3 s, a detection limit of 3.3 µM, excellent repeatability with a relative standard deviation of 0.82%, long-term stability of 95% after four weeks, and an accuracy of 105%. The gold nanoparticles enhanced the electron transfer rate on the electrode. The apparent Michaelis-Menten constant was 4 mM, showing that the enzyme retained catalytic specificity and provided high activity for p-benzoquinone. © 2016, Taylor & Francis.

Eser Adı
[dc.title]
Electrochemical, continuous-flow determination of p-benzoquinone on a gold nanoparticles poly(propylene-co-imidazole) modified gold electrode
Yazar
[dc.contributor.author]
Korkut, Şeyda
Yazar
[dc.contributor.author]
Uzunçar, Sinan
Yazar
[dc.contributor.author]
Kılıç, Muhammet Samet
Yazar
[dc.contributor.author]
Hazer, Baki
Yayın Yılı
[dc.date.issued]
2016
Yayıncı
[dc.publisher]
Taylor and Francis Inc.
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
A novel continuous flow biosensor based on gold nanoparticles and poly(propylene-co-imidazole) was developed for the online determination of p-benzoquinone. The amperometric response was measured as a function of p-benzoquinone concentration at an applied potential of -50 mV. The hydrogen peroxide concentration was optimized and fixed at 1 mM in samples. The mass transfer resistance of the copolymer film was minimized, and the flow cell was regenerated quickly at 1 mL/min. The resulting device provided good analytical performance based on a linear dynamic range from 5–100 µM, a short response time of 3 s, a detection limit of 3.3 µM, excellent repeatability with a relative standard deviation of 0.82%, long-term stability of 95% after four weeks, and an accuracy of 105%. The gold nanoparticles enhanced the electron transfer rate on the electrode. The apparent Michaelis-Menten constant was 4 mM, showing that the enzyme retained catalytic specificity and provided high activity for p-benzoquinone. © 2016, Taylor & Francis.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Continuous flow biosensor
Konu Başlıkları
[dc.subject]
gold nanoparticle
Konu Başlıkları
[dc.subject]
horseradish peroxidase
Konu Başlıkları
[dc.subject]
p-benzoquinone
Künye
[dc.identifier.citation]
Korkut, S., Uzuncar, S., Kilic, M. S. ve Hazer, B. (2016). Electrochemical, continuous-flow determination of p -benzoquinone on a gold nanoparticles poly(Propylene- co -imidazole) modified gold electrode. Instrumentation Science & Technology, 44(6), 614-628. doi:10.1080/10739149.2016.1184161
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1073-9149
İlk Sayfa Sayısı
[dc.identifier.startpage]
614
Son Sayfa Sayısı
[dc.identifier.endpage]
628
Dergi Adı
[dc.relation.journal]
Instrumentation Science and Technology
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
44
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1080/10739149.2016.1184161
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5588
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p-benzoquinone concentration response transfer provided nanoparticles activity stability long-term standard deviation accuracy relative repeatability excellent detection electron specificity catalytic retained Taylor enzyme Francis constant Michaelis-Menten apparent electrode enhanced showing function optimized peroxide hydrogen potential applied
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