An electrochemical biosensor for direct detection of DNA using polystyrene-g-soya oil-g-imidazole graft copolymer

A label-free electrochemical DNA biosensor was developed through the attachment of polystyrene-g-soya oil-g-imidazole graft copolymer (PS-PSyIm) onto modified graphene oxide (GO) electrodeposited on glassy carbon electrode (GC). GC/GO electrode was initially functionalised via electrochemical reduction of 4-nitrobenzene diazonium salt, followed by the electrochemical reduction of NO2 to NH2. Subsequent to the electrochemical deposition of gold nanoparticles on modified surface, the attachment of the PS-PSyIm graft copolymer on the resulting electrode was achieved. The interaction of PS-PSyIm with DNA at the bare glassy carbon electrode was studied by cyclic voltammetry technique, and it was found that interaction predominantly takes place through intercalation mode. The selectivity of developed DNA biosensor was also explored by DPV on the basis of considering hybridisation event with non-complementary, one-base mismatched DNA and complementary target DNA sequence. Large decrease in the peak current was found upon the addition of complementary target DNA. The sensitivity of the developed DNA biosensor was also investigated, and detection limit was found to be 1.20 nmol L-1. © 2017, Springer-Verlag Berlin Heidelberg.

Dergi Adı Journal of Solid State Electrochemistry
Dergi Cilt Bilgisi 21
Dergi Sayısı 5
Sayfalar 1397 - 1405
Yayın Yılı 2017
Eser Adı
[dc.title]
An electrochemical biosensor for direct detection of DNA using polystyrene-g-soya oil-g-imidazole graft copolymer
Yazar
[dc.contributor.author]
Koçak, İzzet
Yazar
[dc.contributor.author]
Şanal, Timur
Yazar
[dc.contributor.author]
Hazer, Baki
Yayın Yılı
[dc.date.issued]
2017
Yayıncı
[dc.publisher]
Springer New York LLC
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
A label-free electrochemical DNA biosensor was developed through the attachment of polystyrene-g-soya oil-g-imidazole graft copolymer (PS-PSyIm) onto modified graphene oxide (GO) electrodeposited on glassy carbon electrode (GC). GC/GO electrode was initially functionalised via electrochemical reduction of 4-nitrobenzene diazonium salt, followed by the electrochemical reduction of NO2 to NH2. Subsequent to the electrochemical deposition of gold nanoparticles on modified surface, the attachment of the PS-PSyIm graft copolymer on the resulting electrode was achieved. The interaction of PS-PSyIm with DNA at the bare glassy carbon electrode was studied by cyclic voltammetry technique, and it was found that interaction predominantly takes place through intercalation mode. The selectivity of developed DNA biosensor was also explored by DPV on the basis of considering hybridisation event with non-complementary, one-base mismatched DNA and complementary target DNA sequence. Large decrease in the peak current was found upon the addition of complementary target DNA. The sensitivity of the developed DNA biosensor was also investigated, and detection limit was found to be 1.20 nmol L-1. © 2017, Springer-Verlag Berlin Heidelberg.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
DNA biosensor
Konu Başlıkları
[dc.subject]
Gold nanoparticles
Konu Başlıkları
[dc.subject]
Graphene oxide
Konu Başlıkları
[dc.subject]
Soya oil
Künye
[dc.identifier.citation]
Kocak, I., Şanal, T. ve Hazer, B. (2017). An electrochemical biosensor for direct detection of DNA using polystyrene-g-soya oil-g-imidazole graft copolymer. Journal of Solid State Electrochemistry, 21(5), 1397–1405. doi:10.1007/s10008-017-3504-8
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1432-8488
İlk Sayfa Sayısı
[dc.identifier.startpage]
1397
Son Sayfa Sayısı
[dc.identifier.endpage]
1405
Dergi Adı
[dc.relation.journal]
Journal of Solid State Electrochemistry
Dergi Sayısı
[dc.identifier.issue]
5
Dergi Cilt Bilgisi
[dc.identifier.volume]
21
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1007/s10008-017-3504-8
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4198
Tam Metin İndirmek için tıklayın Ön izleme
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
22
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 08:30
Google Kontrol
Tıklayınız
electrochemical electrode developed biosensor PS-PSyIm reduction complementary carbon glassy interaction through attachment modified copolymer target mismatched one-base non-complementary hybridisation selectivity considering explored addition sequence Heidelberg Berlin Springer-Verlag 20 nmol L-1 detection investigated sensitivity current decrease intercalation diazonium
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms