The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella

Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1,250, or 2,500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on diets with 156-and 620-ppm BA doses yielded increased SOD activity but 1,250- and 2,500-ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity. However, they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1,250- and 2,500-ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. In contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management. © 2007 Wiley-Liss, Inc.

Dergi Adı Archives of Insect Biochemistry and Physiology
Dergi Cilt Bilgisi 66
Dergi Sayısı 1
Sayfalar 23 - 31
Yayın Yılı 2007
Eser Adı
[dc.title]
The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella
Yazar
[dc.contributor.author]
Hyršl, Pavel
Yazar
[dc.contributor.author]
Büyükgüzel, Ender
Yazar
[dc.contributor.author]
Büyükgüzel, Kemal
Yayın Yılı
[dc.date.issued]
2007
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1,250, or 2,500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on diets with 156-and 620-ppm BA doses yielded increased SOD activity but 1,250- and 2,500-ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity. However, they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1,250- and 2,500-ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. In contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management. © 2007 Wiley-Liss, Inc.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Antioxidant enzymes
Konu Başlıkları
[dc.subject]
Boric acid
Konu Başlıkları
[dc.subject]
Fat body
Konu Başlıkları
[dc.subject]
Galleria mellonella
Konu Başlıkları
[dc.subject]
Hemolymph
Konu Başlıkları
[dc.subject]
Malondialdehyde
Konu Başlıkları
[dc.subject]
Survivorship
Künye
[dc.identifier.citation]
Hyršl, P., Büyükgüzel, E. and Büyükgüzel, K. (2007), The effects of boric acid‐induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella. Arch. Insect Biochem. Physiol., 66: 23-31. doi:10.1002/arch.20194
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0739-4462
İlk Sayfa Sayısı
[dc.identifier.startpage]
23
Son Sayfa Sayısı
[dc.identifier.endpage]
31
Dergi Adı
[dc.relation.journal]
Archives of Insect Biochemistry and Physiology
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
66
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1002/arch.20194
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/8011
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larval increased hemolymph activity significantly decreased 500-ppm larvae glutathione oxidative development stress reared instar toxicity related yielded management Wiley-Liss resulted contrast prolonged mortality concentrations lowest 620-ppm survival concentration affected Dietary However longevity shortened treatments dietary
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