N-acetyl-cysteine improves anastomotic wound healing after radiotherapy in rats

Aim: This study was designed to determine the effects of intraperitoneally or orally administered N-acetylcysteine (NAC) on anastomotic healing of irradiated rats. Methods: Thirty-two male Wistar albino rats were randomized into four groups containing 8 rats each: I; standard resection plus anastomosis, II; radiation plus standard resection plus anastomosis, III; radiation plus standard resection plus anastomosis plus oral NAC, IV; radiation plus standard resection plus anastomosis plus intraperitoneal NAC. Four types of assessment were performed: bursting pressure, hydroxiproline (OHP) content, histopathology, and biochemical evaluation, including serum malondialdehyde (MDA), advanced oxidation protein products (AOPP), reduced glutathione (GSH) and superoxide dismutase (SOD) activities. Results: Group comparisons demonstrated that bursting pressure was significantly higher in NAC treated rats. The mean tissue OHP concentration in the anastomotic tissue was significantly lower in irradiated rats (group II) than in the other groups. NAC treatment caused increased activity of SOD and GSH. In contrast, MDA levels were found to be decreased in groups III and IV. Histopathological analysis revealed that NAC administration, either orally or intraperitoneally, leads to a better anastomotic healing in terms of reepithelialization, perianastomotic fibrosis, ischemic necrosis, and muscle layer destruction. Conclusion: The present study supports the hypothesis that NAC administration alleviates the negative effects of radiotherapy on anastomotic healing. Nevertheless, the underlying mechanisms responsible for this protective effect is unknown today. © 2011 Informa Healthcare USA, Inc.

Yazar Demir E.O.
Cakmak G.K.
Bakkal, Bekir Hakan
Turkcu U.O.
Kandemir N.
Demir A.S.
Tascilar O.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/6712
Tek Biçim Adres 10.3109/08941939.2011.560237
Konu Başlıkları anastomosis
n-acetylcysteine
radiation
rat
Koleksiyonlar Fakülteler
Tıp Fakültesi
Cerrahi Bilimler Bölümü
Makale Koleksiyonu (Cerrahi Bilimler Bölümü)
Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Journal of Investigative Surgery
Dergi Cilt Bilgisi 24
Dergi Sayısı 4
Sayfalar 151 - 158
Yayın Yılı 2011
Eser Adı
[dc.title]
N-acetyl-cysteine improves anastomotic wound healing after radiotherapy in rats
Yazar
[dc.contributor.author]
Demir E.O.
Yazar
[dc.contributor.author]
Cakmak G.K.
Yazar
[dc.contributor.author]
Bakkal, Bekir Hakan
Yazar
[dc.contributor.author]
Turkcu U.O.
Yazar
[dc.contributor.author]
Kandemir N.
Yazar
[dc.contributor.author]
Demir A.S.
Yazar
[dc.contributor.author]
Tascilar O.
Yayın Yılı
[dc.date.issued]
2011
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Aim: This study was designed to determine the effects of intraperitoneally or orally administered N-acetylcysteine (NAC) on anastomotic healing of irradiated rats. Methods: Thirty-two male Wistar albino rats were randomized into four groups containing 8 rats each: I; standard resection plus anastomosis, II; radiation plus standard resection plus anastomosis, III; radiation plus standard resection plus anastomosis plus oral NAC, IV; radiation plus standard resection plus anastomosis plus intraperitoneal NAC. Four types of assessment were performed: bursting pressure, hydroxiproline (OHP) content, histopathology, and biochemical evaluation, including serum malondialdehyde (MDA), advanced oxidation protein products (AOPP), reduced glutathione (GSH) and superoxide dismutase (SOD) activities. Results: Group comparisons demonstrated that bursting pressure was significantly higher in NAC treated rats. The mean tissue OHP concentration in the anastomotic tissue was significantly lower in irradiated rats (group II) than in the other groups. NAC treatment caused increased activity of SOD and GSH. In contrast, MDA levels were found to be decreased in groups III and IV. Histopathological analysis revealed that NAC administration, either orally or intraperitoneally, leads to a better anastomotic healing in terms of reepithelialization, perianastomotic fibrosis, ischemic necrosis, and muscle layer destruction. Conclusion: The present study supports the hypothesis that NAC administration alleviates the negative effects of radiotherapy on anastomotic healing. Nevertheless, the underlying mechanisms responsible for this protective effect is unknown today. © 2011 Informa Healthcare USA, Inc.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
anastomosis
Konu Başlıkları
[dc.subject]
n-acetylcysteine
Konu Başlıkları
[dc.subject]
radiation
Konu Başlıkları
[dc.subject]
rat
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
0894-1939
İlk Sayfa Sayısı
[dc.identifier.startpage]
151
Son Sayfa Sayısı
[dc.identifier.endpage]
158
Dergi Adı
[dc.relation.journal]
Journal of Investigative Surgery
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
24
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.3109/08941939.2011.560237
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/6712
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resection anastomosis standard anastomotic groups healing radiation administration bursting pressure significantly tissue effects orally intraperitoneally irradiated increased revealed reepithelialization better either treatment Histopathological analysis decreased activity perianastomotic contrast caused levels fibrosis responsible Healthcare Informa unknown
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