The effect of erythropoietin on anastomotic healing of irradiated rats

Aim: The aim of the present study is to evaluate the possible protective effects of erythropoietin (EPO) on anastomotic wound healing after preoperative radiotherapy according to its pleiotropic mechanism of action. Methods: Thirty-two male Wistar albino rats were randomized into four groups containing eight rats each: ANAS group, standard resection plus anastomosis; RT+ANAS group, radiation plus standard resection plus anastomosis; ANAS+EPO group, standard resection plus anastomosis plus EPO; RT+ANAS+EPO, radiation plus standard resection plus anastomosis plus EPO. All animals were sacrificed by cardiac puncture, and anastomotic healing was measured by bursting pressure, hydroxyproline (OHP) levels, myeloperoxidase (MPO) activity and histopathological evaluations. Malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-?), and matrix metalloproteinase-9 (MMP-9) were also measured in serum specimens. Results: OHP levels in the RT+ANAS + EPO group were significantly increased compared with other groups (p <.05). In contrast, MPO activity in the RT+ANAS+EPO group was significantly decreased compared with other groups (p <.05). Serum MDA levels were found to be decreased in the ANAS+EPO and RT+ANAS+EPO groups (p <.05). Group comparisons demonstrated that bursting pressure was significantly higher in EPO treated rats (p <.05). The histopathology results revealed that EPO treatment improves anastomotic wound healing though decreased necrosis and inflammatory cell infiltration and increased fibroblast activity. Conclusion: The findings of the present study indicate that EPO contributes to wound healing and the strength of colon anastomosis following radiation due to its antioxidant and anti-inflammatory effects, but further studies are needed to explore the significance of these effects. © 2012 Informa Healthcare USA, Inc.

Dergi Adı Journal of Investigative Surgery
Dergi Cilt Bilgisi 25
Dergi Sayısı 2
Sayfalar 127 - 135
Yayın Yılı 2012
Eser Adı
[dc.title]
The effect of erythropoietin on anastomotic healing of irradiated rats
Yazar
[dc.contributor.author]
Turkcu U.O.
Yazar
[dc.contributor.author]
Cakmak G.K.
Yazar
[dc.contributor.author]
Demir E.O.
Yazar
[dc.contributor.author]
Bakkal, Bekir Hakan
Yazar
[dc.contributor.author]
Oner M.O.
Yazar
[dc.contributor.author]
Okyay R.D.
Yazar
[dc.contributor.author]
Bassorgun I.C.
Yayın Yılı
[dc.date.issued]
2012
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Aim: The aim of the present study is to evaluate the possible protective effects of erythropoietin (EPO) on anastomotic wound healing after preoperative radiotherapy according to its pleiotropic mechanism of action. Methods: Thirty-two male Wistar albino rats were randomized into four groups containing eight rats each: ANAS group, standard resection plus anastomosis; RT+ANAS group, radiation plus standard resection plus anastomosis; ANAS+EPO group, standard resection plus anastomosis plus EPO; RT+ANAS+EPO, radiation plus standard resection plus anastomosis plus EPO. All animals were sacrificed by cardiac puncture, and anastomotic healing was measured by bursting pressure, hydroxyproline (OHP) levels, myeloperoxidase (MPO) activity and histopathological evaluations. Malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-?), and matrix metalloproteinase-9 (MMP-9) were also measured in serum specimens. Results: OHP levels in the RT+ANAS + EPO group were significantly increased compared with other groups (p <.05). In contrast, MPO activity in the RT+ANAS+EPO group was significantly decreased compared with other groups (p <.05). Serum MDA levels were found to be decreased in the ANAS+EPO and RT+ANAS+EPO groups (p <.05). Group comparisons demonstrated that bursting pressure was significantly higher in EPO treated rats (p <.05). The histopathology results revealed that EPO treatment improves anastomotic wound healing though decreased necrosis and inflammatory cell infiltration and increased fibroblast activity. Conclusion: The findings of the present study indicate that EPO contributes to wound healing and the strength of colon anastomosis following radiation due to its antioxidant and anti-inflammatory effects, but further studies are needed to explore the significance of these effects. © 2012 Informa Healthcare USA, Inc.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Anastomosis
Konu Başlıkları
[dc.subject]
Erythropoietin
Konu Başlıkları
[dc.subject]
Healing
Konu Başlıkları
[dc.subject]
Inflammation
Konu Başlıkları
[dc.subject]
Oxidative stress
Konu Başlıkları
[dc.subject]
Radiation
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
0894-1939
Sponsor YAYINCI
[dc.description.sponsorship]
Oct.34
Sponsor YAYINCI
[dc.description.sponsorship]
This study was supported by the Research Fund of the Mugla University (No: 10/34).
İlk Sayfa Sayısı
[dc.identifier.startpage]
127
Son Sayfa Sayısı
[dc.identifier.endpage]
135
Dergi Adı
[dc.relation.journal]
Journal of Investigative Surgery
Dergi Sayısı
[dc.identifier.issue]
2
Dergi Cilt Bilgisi
[dc.identifier.volume]
25
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.3109/08941939.2011.611583
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/7933
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anastomosis resection groups healing standard radiation RT+ANAS+EPO decreased significantly levels activity effects anastomotic increased compared necrosis RT+ANAS ANAS+EPO measured bursting pressure present explore demonstrated significance comparisons higher contrast Informa Healthcare needed treated infiltration inflammatory fibroblast
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