The effect of sildenafil on an animal model for ischemic colitis

  • Yazar İrkörücü, Oktay
    Taşçılar, Öğe
    Çakmak, Güldeniz Karadeniz
    Karakaya, Kemal
    Emre, Ali Uğur
    Uçan, Bülent Hamdi
    Bahadır, Burak
    Açıkgöz, Seferden
    Ankaralı, Handan
    Uğurbaş, Ebru
    Cömert, Mustafa
  • Yayın Türü Article
  • Yayın Yılı 2008
  • Yayıncı SPRINGER
  • Dergi Adı DIGESTIVE DISEASES AND SCIENCES 53, ( 6 ), pp.1618 - 1623
  • Tek Biçim Adres https://hdl.handle.net/20.500.12628/3529
  • Konu Başlıkları ischemic colitis
    ischemic colon
    PDE inhibitor
    sildenafil

Introduction Sildenafil both enhances vasodilatation by relaxing the smooth muscle in the vessels and inhibits platelet aggregation. We have therefore examined the potential benefits of sildenafil on an animal model for ischemic colitis (IC). Methods Twenty-eight female Wistar albino rats weighing 250-300 g were randomized into three experimental groups as follows: in Group 1, animals were sham operated (n = 8) and received tap water; in Groups 2 and 3, the rats underwent a standardized surgical procedure to induce IC (n = 10 in each group). Group 2 animals served as the controls, receiving only tap water, while Group 3 animals received 10 mg/kg sildenafil per day as a single dose for a 3-day period. All animals were sacrificed 72 h after devascularization. To determine the severity of the ischemia, we scored the macroscopically visible damage, measured the ischemic area and scored the histopathology. Tissue malondialdehyde levels were also evaluated. Results The mean area of ischemic changes were 116.80 +/- 189.93 and 0.55 +/- 1.01 mm(2) in Group 2 and 3 animals, respectively (p = 0.0001), while the macroscopically mean visible damage score decreased to 0.66 +/- 0.70 (p = 0.0001) for Group 3 animals. The Chiu scores were 0.00, 3.80 +/- 0.91 and 2.66 +/- 1.00 in Group 1, 2 and 3 animals, respectively. There was a statistically significant difference between Group 2 and 3 animals (p = 0.017). Conclusions Our findings support the view that sildenafil leads to a improvement in IC due to its well-known effects on the vascular smooth muscle and on the microcirculatory hemodynamics.

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Eser Adı
(dc.title)
The effect of sildenafil on an animal model for ischemic colitis
Yazar
(dc.contributor.author)
İrkörücü, Oktay
Yazar
(dc.contributor.author)
Taşçılar, Öğe
Yazar
(dc.contributor.author)
Çakmak, Güldeniz Karadeniz
Yazar
(dc.contributor.author)
Karakaya, Kemal
Yazar
(dc.contributor.author)
Emre, Ali Uğur
Yazar
(dc.contributor.author)
Uçan, Bülent Hamdi
Yazar
(dc.contributor.author)
Bahadır, Burak
Yazar
(dc.contributor.author)
Açıkgöz, Seferden
Yazar
(dc.contributor.author)
Ankaralı, Handan
Yazar
(dc.contributor.author)
Uğurbaş, Ebru
Yazar
(dc.contributor.author)
Cömert, Mustafa
Yayın Yılı
(dc.date.issued)
2008
Yayıncı
(dc.publisher)
SPRINGER
Yayın Türü
(dc.type)
article
Özet
(dc.description.abstract)
Introduction Sildenafil both enhances vasodilatation by relaxing the smooth muscle in the vessels and inhibits platelet aggregation. We have therefore examined the potential benefits of sildenafil on an animal model for ischemic colitis (IC). Methods Twenty-eight female Wistar albino rats weighing 250-300 g were randomized into three experimental groups as follows: in Group 1, animals were sham operated (n = 8) and received tap water; in Groups 2 and 3, the rats underwent a standardized surgical procedure to induce IC (n = 10 in each group). Group 2 animals served as the controls, receiving only tap water, while Group 3 animals received 10 mg/kg sildenafil per day as a single dose for a 3-day period. All animals were sacrificed 72 h after devascularization. To determine the severity of the ischemia, we scored the macroscopically visible damage, measured the ischemic area and scored the histopathology. Tissue malondialdehyde levels were also evaluated. Results The mean area of ischemic changes were 116.80 +/- 189.93 and 0.55 +/- 1.01 mm(2) in Group 2 and 3 animals, respectively (p = 0.0001), while the macroscopically mean visible damage score decreased to 0.66 +/- 0.70 (p = 0.0001) for Group 3 animals. The Chiu scores were 0.00, 3.80 +/- 0.91 and 2.66 +/- 1.00 in Group 1, 2 and 3 animals, respectively. There was a statistically significant difference between Group 2 and 3 animals (p = 0.017). Conclusions Our findings support the view that sildenafil leads to a improvement in IC due to its well-known effects on the vascular smooth muscle and on the microcirculatory hemodynamics.
Açıklama
(dc.description)
WOS: 000255955700024
Açıklama
(dc.description)
PubMed: 17932755
Kayıt Giriş Tarihi
(dc.date.accessioned)
2019-12-23

(dc.date.available)
2019-12-23
Yayın Dili
(dc.language.iso)
eng
Konu Başlıkları
(dc.subject)
ischemic colitis
Konu Başlıkları
(dc.subject)
ischemic colon
Konu Başlıkları
(dc.subject)
PDE inhibitor
Konu Başlıkları
(dc.subject)
sildenafil
Künye
(dc.identifier.citation)
Irkorucu, O., Tascilar, O., Cakmak, G. K., Karakaya, K., Emre, A. U., Ucan, B. H., … Comert, M. (2008). The effect of sildenafil on an animal model for ischemic colitis. Digestive Diseases and Sciences, 53(6), 1618-1623. doi:10.1007/s10620-007-0033-9
Haklar
(dc.rights)
info:eu-repo/semantics/closedAccess
ISSN
(dc.identifier.issn)
0163-2116
İlk Sayfa Sayısı
(dc.identifier.startpage)
1618
Son Sayfa Sayısı
(dc.identifier.endpage)
1623
Dergi Adı
(dc.relation.journal)
DIGESTIVE DISEASES AND SCIENCES
Dergi Sayısı
(dc.identifier.issue)
6
Dergi Cilt Bilgisi
(dc.identifier.volume)
53
Tek Biçim Adres
(dc.identifier.uri)
https://dx.doi.org/10.1007/s10620-007-0033-9
Tek Biçim Adres
(dc.identifier.uri)
https://hdl.handle.net/20.500.12628/3529
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