Protective effects of erythropoietin against acute lung injury in a rat model of acute necrotizing pancreatitis

AIM: To investigate the effect of exogenous erythropoietin (EPO) administration on acute lung injury (ALI) in an experimental model of sodium taurodeoxycholate-induced acute necrotizing pancreatitis (ANP). METHODS: Forty-seven male Wistar albino rats were randomly divided into 7 groups: sham group (n = 5), 3 ANP groups (n = 7 each) and 3 EPO groups (n = 7 each). ANP was induced by retrograde infusion of 5% sodium taurodeoxycholate into the common bile duct. Rats in EPO groups received 1000 U/kg intramuscular EPO immediately after induction of ANP Rats in ANP groups were given 1 mL normal saline instead. All animals were sacrificed at postoperative 24 h, 48 h and 72 h. Serum amilase, IL-2, IL-6 and lung tissue malondialdehyde (MDA) were measured. Pleural effusion volume and lung/body weight (LW/BW) ratios were calculated. Tissue levels of TNF-alpha, IL-2 and IL-6 were screened immunohistochemically. Additionally, ox-LDL accumulation was assessed with immune-fluorescent staining. Histopathological alterations in the lungs were also scored. RESULTS: The mean pleural effusion volume, calculated LW/BW ratio, serum IL-6 and lung tissue MDA levels were significantly lower in EPO groups than in ANP groups. No statistically significant difference was observed in either serum or tissue values of IL-2 among the groups. The level of tumor necrosis factor-alpha (TNF-alpha) and IL-6 and accumulation of ox-LDL were evident in the lung tissues of ANP groups when compared to EPO groups, particularly at 72 h. Histopathological evaluation confirmed the improvement in lung injury parameters after exogenous EPO administration, particularly, at 48 h and 72 h. CONCLUSION: EPO administration leads to a significant decrease in ALI parameters by inhibiting polymorphonuclear leukocyte (PMNL) accumulation, decreasing the levels of proinflammatory cytokines in circulation, preserving microvascular endothelial cell integrity and reducing oxidative stress-associated lipid peroxidation and therefore, can be regarded as a cytoprotective agent in ANP-induced ALI. (c) 2007 WJG. All rights reserved.

Dergi Adı WORLD JOURNAL OF GASTROENTEROLOGY
Dergi Cilt Bilgisi 13
Dergi Sayısı 46
Sayfalar 6172 - 6182
Yayın Yılı 2007
Eser Adı
[dc.title]
Protective effects of erythropoietin against acute lung injury in a rat model of acute necrotizing pancreatitis
Yazar
[dc.contributor.author]
Taşçılar, Öğe
Yazar
[dc.contributor.author]
Çakmak, Güldeniz Karadeniz
Yazar
[dc.contributor.author]
Tekin, İshak Özel
Yazar
[dc.contributor.author]
Emre, Ali Uğur
Yazar
[dc.contributor.author]
Uçan, Bülent Hamdi
Yazar
[dc.contributor.author]
Bahadır, Burak
Yazar
[dc.contributor.author]
Açıkgöz, Serefden
Yazar
[dc.contributor.author]
İrkörücü, Oktay
Yazar
[dc.contributor.author]
Karakaya, Kemal
Yazar
[dc.contributor.author]
Balbaloğlu, Hakan
Yazar
[dc.contributor.author]
Kertiş, Gürkan
Yazar
[dc.contributor.author]
Ankaralı, Handan
Yazar
[dc.contributor.author]
Cömert, Mustafa
Yayın Yılı
[dc.date.issued]
2007
Yayıncı
[dc.publisher]
BAISHIDENG PUBLISHING GROUP INC
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
AIM: To investigate the effect of exogenous erythropoietin (EPO) administration on acute lung injury (ALI) in an experimental model of sodium taurodeoxycholate-induced acute necrotizing pancreatitis (ANP). METHODS: Forty-seven male Wistar albino rats were randomly divided into 7 groups: sham group (n = 5), 3 ANP groups (n = 7 each) and 3 EPO groups (n = 7 each). ANP was induced by retrograde infusion of 5% sodium taurodeoxycholate into the common bile duct. Rats in EPO groups received 1000 U/kg intramuscular EPO immediately after induction of ANP Rats in ANP groups were given 1 mL normal saline instead. All animals were sacrificed at postoperative 24 h, 48 h and 72 h. Serum amilase, IL-2, IL-6 and lung tissue malondialdehyde (MDA) were measured. Pleural effusion volume and lung/body weight (LW/BW) ratios were calculated. Tissue levels of TNF-alpha, IL-2 and IL-6 were screened immunohistochemically. Additionally, ox-LDL accumulation was assessed with immune-fluorescent staining. Histopathological alterations in the lungs were also scored. RESULTS: The mean pleural effusion volume, calculated LW/BW ratio, serum IL-6 and lung tissue MDA levels were significantly lower in EPO groups than in ANP groups. No statistically significant difference was observed in either serum or tissue values of IL-2 among the groups. The level of tumor necrosis factor-alpha (TNF-alpha) and IL-6 and accumulation of ox-LDL were evident in the lung tissues of ANP groups when compared to EPO groups, particularly at 72 h. Histopathological evaluation confirmed the improvement in lung injury parameters after exogenous EPO administration, particularly, at 48 h and 72 h. CONCLUSION: EPO administration leads to a significant decrease in ALI parameters by inhibiting polymorphonuclear leukocyte (PMNL) accumulation, decreasing the levels of proinflammatory cytokines in circulation, preserving microvascular endothelial cell integrity and reducing oxidative stress-associated lipid peroxidation and therefore, can be regarded as a cytoprotective agent in ANP-induced ALI. (c) 2007 WJG. All rights reserved.
Açıklama
[dc.description]
WOS: 000251840200007
Açıklama
[dc.description]
PubMed: 18069756
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
erythropoietin
Konu Başlıkları
[dc.subject]
acute pancreatitis
Konu Başlıkları
[dc.subject]
acute lung injury
Konu Başlıkları
[dc.subject]
acute respiratory distress syndrome
Konu Başlıkları
[dc.subject]
cytokine
Künye
[dc.identifier.citation]
Tascilar, O., Cakmak, G. K., Tekin, I. O., Emre, A. U., Ucan, B. H., Bahadir, B., … Comert, M. (2007). Protective effects of erythropoietin against acute lung injury in a rat model of acute necrotizing pancreatitis. World Journal of Gastroenterology, 13(46), 6172-6182. doi:10.3748/wjg.13.6172
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
1007-9327
ISSN
[dc.identifier.issn]
2219-2840
İlk Sayfa Sayısı
[dc.identifier.startpage]
6172
Son Sayfa Sayısı
[dc.identifier.endpage]
6182
Dergi Adı
[dc.relation.journal]
WORLD JOURNAL OF GASTROENTEROLOGY
Dergi Sayısı
[dc.identifier.issue]
46
Dergi Cilt Bilgisi
[dc.identifier.volume]
13
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.3748/wjg.13.6172
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3184
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groups levels tissue accumulation administration parameters particularly significant sodium exogenous ox-LDL calculated volume Histopathological effusion injury factor-alpha pleural necrosis (TNF-alpha) significantly statistically difference observed values either evident tissues regarded oxidative stress-associated peroxidation therefore integrity cytoprotective
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