Comparison of Direct and Remote Ischaemic Preconditioning of Renal Ischaemia Reperfusion Injury in Rats

Objective: One of the methods that can be used to prevent ischaemia reperfusion (IR) injury is ischaemic preconditioning. The aim of this study was to evaluate and compare the effects of remote and direct ischaemic preconditioning (RIPC and DIPC) histopathologically in the rat renal IR injury model. Methods: After obtaining an approval from the Dokuz Eylul University School of Medicine Ethics Committee, 28 Wistar Albino male rats were divided into four groups. In Group I (Sham, n=7), laparotomy and left renal pedicle dissection were performed, but nothing else was done. In Group II (IR, n=7), after 45 minutes of left renal pedicle occlusion, reperfusion lasting 4 hours was performed. In Group III (DIPC+IR, n=7), after four cycles of ischaemic preconditioning applied to the left kidney, renal IR was performed. In Group IV (RIPC+IR, n=7), after three cycles of ischaemic preconditioning applied to the left hind leg, renal IR was performed. All rats were sacrificed, and the left kidney was processed for conventional histopathology. Results: The histopathological injury score of the kidney was significantly lower in the sham group compared with the other groups (p<0.01). The injury scores of the DIPC+IR and RIPC+IR groups were significantly lower than in the IR group (p<0.05). In the RIPC+IR group, the injury score for erythrocyte extravasation was found to be significantly lower than in the DIPC+IR group (p<0.05). Conclusion: In the present study, it was demonstrated that both DIPC and RIPC decreased renal IR injury, but RIPC was found to be more effective than DIPC. This protective effect requiresfurther detailed experimental and clinical studies.

Yazar Oral, Keziban
Akan, Mert
Ozkardesler, Sevda
Boztas, Nilay
Ergur, Bekir Ugur
Guneli, Mehmet Ensari
Olguner, Cimen
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2267
Tek Biçim Adres 10.5152/TJAR.2018.07992
Konu Başlıkları Kidney
reperfusion injury
ischaemic preconditioning
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı TURKISH JOURNAL OF ANAESTHESIOLOGY AND REANIMATION
Dergi Cilt Bilgisi 46
Dergi Sayısı 6
Sayfalar 453 - 461
Yayın Yılı 2018
Eser Adı
[dc.title]
Comparison of Direct and Remote Ischaemic Preconditioning of Renal Ischaemia Reperfusion Injury in Rats
Yazar
[dc.contributor.author]
Oral, Keziban
Yazar
[dc.contributor.author]
Akan, Mert
Yazar
[dc.contributor.author]
Ozkardesler, Sevda
Yazar
[dc.contributor.author]
Boztas, Nilay
Yazar
[dc.contributor.author]
Ergur, Bekir Ugur
Yazar
[dc.contributor.author]
Guneli, Mehmet Ensari
Yazar
[dc.contributor.author]
Olguner, Cimen
Yayın Yılı
[dc.date.issued]
2018
Yayıncı
[dc.publisher]
AVES
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Objective: One of the methods that can be used to prevent ischaemia reperfusion (IR) injury is ischaemic preconditioning. The aim of this study was to evaluate and compare the effects of remote and direct ischaemic preconditioning (RIPC and DIPC) histopathologically in the rat renal IR injury model. Methods: After obtaining an approval from the Dokuz Eylul University School of Medicine Ethics Committee, 28 Wistar Albino male rats were divided into four groups. In Group I (Sham, n=7), laparotomy and left renal pedicle dissection were performed, but nothing else was done. In Group II (IR, n=7), after 45 minutes of left renal pedicle occlusion, reperfusion lasting 4 hours was performed. In Group III (DIPC+IR, n=7), after four cycles of ischaemic preconditioning applied to the left kidney, renal IR was performed. In Group IV (RIPC+IR, n=7), after three cycles of ischaemic preconditioning applied to the left hind leg, renal IR was performed. All rats were sacrificed, and the left kidney was processed for conventional histopathology. Results: The histopathological injury score of the kidney was significantly lower in the sham group compared with the other groups (p<0.01). The injury scores of the DIPC+IR and RIPC+IR groups were significantly lower than in the IR group (p<0.05). In the RIPC+IR group, the injury score for erythrocyte extravasation was found to be significantly lower than in the DIPC+IR group (p<0.05). Conclusion: In the present study, it was demonstrated that both DIPC and RIPC decreased renal IR injury, but RIPC was found to be more effective than DIPC. This protective effect requiresfurther detailed experimental and clinical studies.
Açıklama
[dc.description]
WOS: 000449525500009
Açıklama
[dc.description]
PubMed: 30505608
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Kidney
Konu Başlıkları
[dc.subject]
reperfusion injury
Konu Başlıkları
[dc.subject]
ischaemic preconditioning
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
2149-0937
ISSN
[dc.identifier.issn]
2149-276X
İlk Sayfa Sayısı
[dc.identifier.startpage]
453
Son Sayfa Sayısı
[dc.identifier.endpage]
461
Dergi Adı
[dc.relation.journal]
TURKISH JOURNAL OF ANAESTHESIOLOGY AND REANIMATION
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
46
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.5152/TJAR.2018.07992
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2267
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
15
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
07 Şubat 2024 20:42
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Tıklayınız
injury preconditioning ischaemic performed significantly groups kidney pedicle DIPC+IR applied cycles RIPC+IR reperfusion erythrocyte experimental processed effect requiresfurther detailed sacrificed (RIPC+IR clinical studies protective conventional present (DIPC+IR scores Conclusion compared effective demonstrated histopathological Results histopathology
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