Experimental Obstructive Jaundice Results in Oxidized Low-Density-Lipoprotein Accumulation in Surgical Wound of Rats

Introduction : Mounting evidence suggests that impaired wound heating is a well-defined consequence in obstructive jaundice and, as redox-regulated processes are relevant to wound healing, it is not unreasonable to suppose that oxidative stress associated with lipid peroxidation in cholestasis might be a systemic phenomenon probably comprising all tissues and organs, including wounds. The aim of the present investigation was to analyse the lipid peroxidation status of surgical wounds, in terms of oxidized low-density-lipoprotein (oxLDL) accumulation in experimental obstructive jaundice. Methods : Sixteen Wistar-Albino rats weighing 200-230 gr were randomly divided into two groups. Group I (n = 8) was designed as the prolonged obstructive jaundice group and was subjected to bile duct ligation. Group II (Sham-control, n = 8) rats underwent laparotomy alone and bile duct was just dissected from the surrounding tissue. Histopathological evaluation. immunohistochemical screening and immunoflourescent staining of the surgical wound was conducted to the bile-duct ligated rats and control group on the 21(st) postoperative day. Results : Wound healing was found to be impaired in jaundiced rats histopathologically. When compared with the control group. significant positive oxLDL staining and intracellular accumulation of TNF-alpha, IL-2 and IL-6 was detected ill the wound sections of the prolonged obstructive jaundice group. Conclusion : Our present data is the first in the literature, indicating significant oxLDL accumulation in surgical wounds of cholestatic rats, which might be one of the results of systemic oxidative stress leading to deficient healing capacity as a consequence of persistent inflammation.

Yazar Cakamak, G. Karadeniz
Tascilar, O.
Tekin, I. O.
Ucan, B. H.
Emre, A. U.
Gun, B. D.
Acikgoz, S.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2625
Konu Başlıkları oxLDL
obstructive jaundice
wound healing
oxidative stress
lipid peroxidation
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı ACTA CHIRURGICA BELGICA
Dergi Cilt Bilgisi 108
Dergi Sayısı 6
Sayfalar 725 - 731
Yayın Yılı 2008
Eser Adı
[dc.title]
Experimental Obstructive Jaundice Results in Oxidized Low-Density-Lipoprotein Accumulation in Surgical Wound of Rats
Yazar
[dc.contributor.author]
Cakamak, G. Karadeniz
Yazar
[dc.contributor.author]
Tascilar, O.
Yazar
[dc.contributor.author]
Tekin, I. O.
Yazar
[dc.contributor.author]
Ucan, B. H.
Yazar
[dc.contributor.author]
Emre, A. U.
Yazar
[dc.contributor.author]
Gun, B. D.
Yazar
[dc.contributor.author]
Acikgoz, S.
Yayın Yılı
[dc.date.issued]
2008
Yayıncı
[dc.publisher]
ACTA MEDICAL BELGICA
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Introduction : Mounting evidence suggests that impaired wound heating is a well-defined consequence in obstructive jaundice and, as redox-regulated processes are relevant to wound healing, it is not unreasonable to suppose that oxidative stress associated with lipid peroxidation in cholestasis might be a systemic phenomenon probably comprising all tissues and organs, including wounds. The aim of the present investigation was to analyse the lipid peroxidation status of surgical wounds, in terms of oxidized low-density-lipoprotein (oxLDL) accumulation in experimental obstructive jaundice. Methods : Sixteen Wistar-Albino rats weighing 200-230 gr were randomly divided into two groups. Group I (n = 8) was designed as the prolonged obstructive jaundice group and was subjected to bile duct ligation. Group II (Sham-control, n = 8) rats underwent laparotomy alone and bile duct was just dissected from the surrounding tissue. Histopathological evaluation. immunohistochemical screening and immunoflourescent staining of the surgical wound was conducted to the bile-duct ligated rats and control group on the 21(st) postoperative day. Results : Wound healing was found to be impaired in jaundiced rats histopathologically. When compared with the control group. significant positive oxLDL staining and intracellular accumulation of TNF-alpha, IL-2 and IL-6 was detected ill the wound sections of the prolonged obstructive jaundice group. Conclusion : Our present data is the first in the literature, indicating significant oxLDL accumulation in surgical wounds of cholestatic rats, which might be one of the results of systemic oxidative stress leading to deficient healing capacity as a consequence of persistent inflammation.
Açıklama
[dc.description]
WOS: 000262975700018
Açıklama
[dc.description]
PubMed: 19241926
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
oxLDL
Konu Başlıkları
[dc.subject]
obstructive jaundice
Konu Başlıkları
[dc.subject]
wound healing
Konu Başlıkları
[dc.subject]
oxidative stress
Konu Başlıkları
[dc.subject]
lipid peroxidation
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0001-5458
İlk Sayfa Sayısı
[dc.identifier.startpage]
725
Son Sayfa Sayısı
[dc.identifier.endpage]
731
Dergi Adı
[dc.relation.journal]
ACTA CHIRURGICA BELGICA
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
108
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2625
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
19
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 21:57
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Tıklayınız
jaundice obstructive healing wounds surgical accumulation control staining present significant systemic peroxidation stress prolonged impaired oxidative consequence dissected postoperative 21(st) surrounding tissue ligated bile-duct conducted Histopathological immunoflourescent evaluation screening immunohistochemical Results Introduction inflammation persistent capacity
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