Contribution of flavonoid antioxidants to the preventive effect of mesna in cyclophosphamide-induced cystitis in rats

Cyclophosphamide (CP) is widely used, alone or in combination with other chemotherapeutic agents, for treatment of neoplastic diseases. Its urotoxicity may cause dose-limiting side-effects, for example hemorrhagic cystitis. The agent most often used to prevent this side-effect is mesna (2-mercaptoethane sulfonate). Overproduction of reactive oxygen species during inflammation is one reason for possible urothelial injury. The aim of this study was to evaluate whether combinations of quercetin and epigallocatechin 3-gallate (EGCG), flavonoid antioxidants and mesna could prevent cystitis induced by cyclophosphamide, better than mesna alone. A total of 38 male Sprague-Dawley rats were divided into five groups. Four groups received single dose of CP (100 mg kg(-1)) intraperitoneally at the same time. Group 2 received CP only, group 3 received mesna (3x21.5 mg kg(-1)), group 4 received a single dose of mesna+EGCG (2x20 mg kg(-1)), and group 5 received a single dose of mesna+quercetin (2x20 mg kg(-1)), before and after CP injection. Group 1 (not treated) served as control. CP injection alone resulted in severe cystitis. Mesna resulted in some, but not full, protection against CP toxicity. Quercetin and catechine, together with mesna, resulted in full protection against CP toxicity, on the basis of histopathology of the urinary bladder. It was concluded that oxidants might be important in the pathogenesis of CP-induced cystitis, and that flavonoid antioxidants, used in addition to mesna, may help to ameliorate bladder damage.

Yazar Ozcan, A
Korkmaz, A
Oter, S
Coskun, O
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2307
Tek Biçim Adres 10.1007/s00204-005-0647-7
Konu Başlıkları cyclophosphamide
cystitis
flavonoids
mesna
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı ARCHIVES OF TOXICOLOGY
Dergi Cilt Bilgisi 79
Dergi Sayısı 8
Sayfalar 461 - 465
Yayın Yılı 2005
Eser Adı
[dc.title]
Contribution of flavonoid antioxidants to the preventive effect of mesna in cyclophosphamide-induced cystitis in rats
Yazar
[dc.contributor.author]
Ozcan, A
Yazar
[dc.contributor.author]
Korkmaz, A
Yazar
[dc.contributor.author]
Oter, S
Yazar
[dc.contributor.author]
Coskun, O
Yayın Yılı
[dc.date.issued]
2005
Yayıncı
[dc.publisher]
SPRINGER
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Cyclophosphamide (CP) is widely used, alone or in combination with other chemotherapeutic agents, for treatment of neoplastic diseases. Its urotoxicity may cause dose-limiting side-effects, for example hemorrhagic cystitis. The agent most often used to prevent this side-effect is mesna (2-mercaptoethane sulfonate). Overproduction of reactive oxygen species during inflammation is one reason for possible urothelial injury. The aim of this study was to evaluate whether combinations of quercetin and epigallocatechin 3-gallate (EGCG), flavonoid antioxidants and mesna could prevent cystitis induced by cyclophosphamide, better than mesna alone. A total of 38 male Sprague-Dawley rats were divided into five groups. Four groups received single dose of CP (100 mg kg(-1)) intraperitoneally at the same time. Group 2 received CP only, group 3 received mesna (3x21.5 mg kg(-1)), group 4 received a single dose of mesna+EGCG (2x20 mg kg(-1)), and group 5 received a single dose of mesna+quercetin (2x20 mg kg(-1)), before and after CP injection. Group 1 (not treated) served as control. CP injection alone resulted in severe cystitis. Mesna resulted in some, but not full, protection against CP toxicity. Quercetin and catechine, together with mesna, resulted in full protection against CP toxicity, on the basis of histopathology of the urinary bladder. It was concluded that oxidants might be important in the pathogenesis of CP-induced cystitis, and that flavonoid antioxidants, used in addition to mesna, may help to ameliorate bladder damage.
Açıklama
[dc.description]
WOS: 000231353300005
Açıklama
[dc.description]
PubMed: 15800758
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
cyclophosphamide
Konu Başlıkları
[dc.subject]
cystitis
Konu Başlıkları
[dc.subject]
flavonoids
Konu Başlıkları
[dc.subject]
mesna
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0340-5761
İlk Sayfa Sayısı
[dc.identifier.startpage]
461
Son Sayfa Sayısı
[dc.identifier.endpage]
465
Dergi Adı
[dc.relation.journal]
ARCHIVES OF TOXICOLOGY
Dergi Sayısı
[dc.identifier.issue]
8
Dergi Cilt Bilgisi
[dc.identifier.volume]
79
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1007/s00204-005-0647-7
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2307
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
20
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
10 Şubat 2024 02:16
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Tıklayınız
received cystitis kg(-1)) resulted single flavonoid antioxidants protection against toxicity prevent injection bladder groups mesna+EGCG mesna+quercetin before intraperitoneally Cyclophosphamide urinary damage ameliorate addition CP-induced pathogenesis important oxidants concluded histopathology together catechine Quercetin severe control served
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