Discrimination of 2n=60 Spalax leucodon cytotypes (Spalacidae, Rodentia) in Turkey by means of classical and molecular cytogenetic techniques

Comparative studies among seven populations of 2n = 60 S. leucodon employing classic cytogenetics (G-bands, C-bands, AgNOR-staining), fluorochrome staining, and fluorescence in situ hybridization of telomeric and rDNA probes are reported here for the first time. The studied specimens were assigned to two cytotypes: 2n = 60W and 2n = 60R. The basic karyotype of both cytotypes consisted of eight pairs of subtelocentric and 21 pairs of acrocentric autosomes, subtelocentric X and acrocentric Y chromosomes. Both cytotypes had variable numbers of B-chromosomes (1-3) and variable numbers of autosomal arms (NFa = 74-76) caused by amplification (deletion) of heterochromatin short arms in the second pair. The short arms of subtelocentric chromosomes were comprised of heterochromatin in both cytotypes. Nucleolar organizer regions (NORs) and rDNA clusters were detected at telomeric sites of the short arms in pairs Nos. 3, 5, 6, 9, and 13 in cytotype W, and in the short arms of pair No. 6, 8, 12, 13, and 16 in cytotype R. Different locations of rDNA clusters allowed unambiguous discrimination between two S. leucodon cytotypes possessing the same 2n = 60 and similar NFa (74-76) variability. Our findings suggest a high level of chromosomal divergence, which means that it is possible to consider these cytotypes as a well-differentiated, chromosomal lineage within the leucodon group. Copyright (C) 2008 S. Karger AG, Basel

Dergi Adı CYTOGENETIC AND GENOME RESEARCH
Dergi Cilt Bilgisi 122
Dergi Sayısı 2
Sayfalar 139 - 149
Yayın Yılı 2008
Eser Adı
[dc.title]
Discrimination of 2n=60 Spalax leucodon cytotypes (Spalacidae, Rodentia) in Turkey by means of classical and molecular cytogenetic techniques
Yazar
[dc.contributor.author]
Ivanitskaya, E.
Yazar
[dc.contributor.author]
Sözen, Mustafa
Yazar
[dc.contributor.author]
Rashkovetsky, L.
Yazar
[dc.contributor.author]
Matur, Ferhat
Yazar
[dc.contributor.author]
Nevo, E.
Yayın Yılı
[dc.date.issued]
2008
Yayıncı
[dc.publisher]
KARGER
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Comparative studies among seven populations of 2n = 60 S. leucodon employing classic cytogenetics (G-bands, C-bands, AgNOR-staining), fluorochrome staining, and fluorescence in situ hybridization of telomeric and rDNA probes are reported here for the first time. The studied specimens were assigned to two cytotypes: 2n = 60W and 2n = 60R. The basic karyotype of both cytotypes consisted of eight pairs of subtelocentric and 21 pairs of acrocentric autosomes, subtelocentric X and acrocentric Y chromosomes. Both cytotypes had variable numbers of B-chromosomes (1-3) and variable numbers of autosomal arms (NFa = 74-76) caused by amplification (deletion) of heterochromatin short arms in the second pair. The short arms of subtelocentric chromosomes were comprised of heterochromatin in both cytotypes. Nucleolar organizer regions (NORs) and rDNA clusters were detected at telomeric sites of the short arms in pairs Nos. 3, 5, 6, 9, and 13 in cytotype W, and in the short arms of pair No. 6, 8, 12, 13, and 16 in cytotype R. Different locations of rDNA clusters allowed unambiguous discrimination between two S. leucodon cytotypes possessing the same 2n = 60 and similar NFa (74-76) variability. Our findings suggest a high level of chromosomal divergence, which means that it is possible to consider these cytotypes as a well-differentiated, chromosomal lineage within the leucodon group. Copyright (C) 2008 S. Karger AG, Basel
Açıklama
[dc.description]
WOS: 000261979300007
Açıklama
[dc.description]
PubMed: 19096209
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1424-8581
ISSN
[dc.identifier.issn]
1424-859X
Sponsor YAYINCI
[dc.description.sponsorship]
Research Authority of Haifa University
Sponsor YAYINCI
[dc.description.sponsorship]
This study was supported by the Research Authority of Haifa University.
İlk Sayfa Sayısı
[dc.identifier.startpage]
139
Son Sayfa Sayısı
[dc.identifier.endpage]
149
Dergi Adı
[dc.relation.journal]
CYTOGENETIC AND GENOME RESEARCH
Dergi Sayısı
[dc.identifier.issue]
2
Dergi Cilt Bilgisi
[dc.identifier.volume]
122
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1159/000163091
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2398
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1159/000163091
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
22
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
01 Mart 2024 17:31
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Tıklayınız
cytotypes leucodon subtelocentric clusters numbers acrocentric chromosomes variable cytotype heterochromatin chromosomal telomeric divergence lineage within locations Different Copyright unambiguous Karger allowed well-differentiated suggest findings variability discrimination (74-76) possible similar consider possessing between Comparative caused specimens
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