N-acetyl cysteine promotes angiogenesis and clearance of free oxygen radicals, thus improving wound healing in an alloxan-induced diabetic mouse model of incisional wound

Background. This study investigated whether N-acetyl cysteine induces any favourable effects on cutaneous incisional wound healing in diabetic and nondiabetic mice. The wounds were assessed using detection of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) expression, and wound-breaking strength (WBS) measurements. Methods. In total, 48 BALB/c mice were used. These were divided into four groups, each consisting of 12 mice. Incisional wounds were made on the back of each mouse. Two of the groups consisted of healthy animals and the other two groups consisted of mice with alloxan-induced diabetes. One group of healthy mice and one group of diabetic mice received intraperitoneal N-acetyl cysteine (NAC) 150 mg/kg for 5 consecutive days, while the other two groups were untreated. On the fifth day all animals were killed, and the WBS, oxidative stress parameters, histopathological and immunohisotchemical results were assessed. Results. Both diabetic and nondiabetic mice receiving NAC had lower levels of oxidative stress markers and higher WBS measurements than untreated counterparts. Conclusions. A mouse model of incisional wound treated with NAC resulted in lower levels of tissue oxidative stress, higher levels of tissue glutathione, and downregulation of iNOS expression coupled with upregulation of VEGF expression, producing an overall favourable clinical outcome of higher WBS and a shorter wound-healing period both in diabetic and nondiabetic mice. Both antioxidant and anti-inflammatory properties of NAC may be involved in this improved healing process for incisional wounds.

Yazar Aktunc, E.
Ozacmak, V. H.
Ozacmak, H. S.
Barut, Figen
Buyukates, M.
Kandemir, O.
Demircan, N.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2976
Tek Biçim Adres 10.1111/j.1365-2230.2010.03823.x
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı CLINICAL AND EXPERIMENTAL DERMATOLOGY
Dergi Cilt Bilgisi 35
Dergi Sayısı 8
Sayfalar 902 - 909
Yayın Yılı 2010
Eser Adı
[dc.title]
N-acetyl cysteine promotes angiogenesis and clearance of free oxygen radicals, thus improving wound healing in an alloxan-induced diabetic mouse model of incisional wound
Yazar
[dc.contributor.author]
Aktunc, E.
Yazar
[dc.contributor.author]
Ozacmak, V. H.
Yazar
[dc.contributor.author]
Ozacmak, H. S.
Yazar
[dc.contributor.author]
Barut, Figen
Yazar
[dc.contributor.author]
Buyukates, M.
Yazar
[dc.contributor.author]
Kandemir, O.
Yazar
[dc.contributor.author]
Demircan, N.
Yayın Yılı
[dc.date.issued]
2010
Yayıncı
[dc.publisher]
WILEY-BLACKWELL
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background. This study investigated whether N-acetyl cysteine induces any favourable effects on cutaneous incisional wound healing in diabetic and nondiabetic mice. The wounds were assessed using detection of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) expression, and wound-breaking strength (WBS) measurements. Methods. In total, 48 BALB/c mice were used. These were divided into four groups, each consisting of 12 mice. Incisional wounds were made on the back of each mouse. Two of the groups consisted of healthy animals and the other two groups consisted of mice with alloxan-induced diabetes. One group of healthy mice and one group of diabetic mice received intraperitoneal N-acetyl cysteine (NAC) 150 mg/kg for 5 consecutive days, while the other two groups were untreated. On the fifth day all animals were killed, and the WBS, oxidative stress parameters, histopathological and immunohisotchemical results were assessed. Results. Both diabetic and nondiabetic mice receiving NAC had lower levels of oxidative stress markers and higher WBS measurements than untreated counterparts. Conclusions. A mouse model of incisional wound treated with NAC resulted in lower levels of tissue oxidative stress, higher levels of tissue glutathione, and downregulation of iNOS expression coupled with upregulation of VEGF expression, producing an overall favourable clinical outcome of higher WBS and a shorter wound-healing period both in diabetic and nondiabetic mice. Both antioxidant and anti-inflammatory properties of NAC may be involved in this improved healing process for incisional wounds.
Açıklama
[dc.description]
WOS: 000283734600017
Açıklama
[dc.description]
PubMed: 20408853
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0307-6938
İlk Sayfa Sayısı
[dc.identifier.startpage]
902
Son Sayfa Sayısı
[dc.identifier.endpage]
909
Dergi Adı
[dc.relation.journal]
CLINICAL AND EXPERIMENTAL DERMATOLOGY
Dergi Sayısı
[dc.identifier.issue]
8
Dergi Cilt Bilgisi
[dc.identifier.volume]
35
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1111/j.1365-2230.2010.03823.x
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2976
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
18
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 08:16
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Tıklayınız
diabetic groups nondiabetic expression incisional levels wounds stress oxidative higher untreated consisted healthy animals tissue measurements assessed N-acetyl favourable cysteine healing receiving anti-inflammatory properties killed involved process Results improved antioxidant immunohisotchemical histopathological parameters results overall
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