Synthesis, characterization, and DNA binding of complexes [Pt(bpy)(pip)](2+) and [Pt(bpy)(hpip)](2+)

Two new platinum(II) complexes, [Pt(bpy)(pip)](NO3)(2) (1) and [Pt(bpy)(hpip)](NO3)(2)center dot 2H(2)O (2) (bpy is 2,2'-bypyridine; pip is 2-phenylimidazo[4,5-f][1,10]phenanthroline; hpip is 2-(2-hydroxyphenyl) imidazo[4,5-f][1,10]phenanthroline), have been synthesized and fully characterized by CHN analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, H-1-NMR spectroscopy, IR spectroscopy (attenuated total reflection), and UV-vis spectroscopy. The DNA-binding behaviors of both complexes have been studied by spectroscopic methods and viscosity measurements, and their ability to inhibit DNA transcription was measured. The results indicate that both complexes show some degree of binding to DNA in an intercalative mode, resulting in intrinsic binding constants of (2.88 +/- A 0.4) x 10(4) and (5.38 +/- A 0.8) x 10(4) for 1 and 2, respectively. The comparatively observed difference in the DNA-binding affinities of the two complexes can be reasonably explained by the presence of intramolecular hydrogen bonding between the ortho phenolic group and the nitrogen atom of the imidazole ring. The extended coplanarity of the hpip ligand due to intramolecular hydrogen bonding may lead to an enhancement of the DNA-binding affinity of the hpip complex. In addition, the complexes can promote photocleavage of pUC19 DNA on irradiation as revealed by the spectroscopic and viscometric measurements, with 2 promoting cleavage of pUC19 DNA at lower concentration. Moreover, increasing concentrations of both complexes inhibited DNA transcription, and as expected 2 was shown to be a better antitumor agent than 1.

Yazar Coban, Burak
Yildiz, Ufuk
Sengul, Abdurrahman
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3423
Tek Biçim Adres 10.1007/s00775-013-0991-7
Konu Başlıkları Pt(II) complex
Polypyridyl ligand
DNA binding
Photocleavage
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Dergi Cilt Bilgisi 18
Dergi Sayısı 4
Sayfalar 461 - 471
Yayın Yılı 2013
Eser Adı
[dc.title]
Synthesis, characterization, and DNA binding of complexes [Pt(bpy)(pip)](2+) and [Pt(bpy)(hpip)](2+)
Yazar
[dc.contributor.author]
Coban, Burak
Yazar
[dc.contributor.author]
Yildiz, Ufuk
Yazar
[dc.contributor.author]
Sengul, Abdurrahman
Yayın Yılı
[dc.date.issued]
2013
Yayıncı
[dc.publisher]
SPRINGER
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Two new platinum(II) complexes, [Pt(bpy)(pip)](NO3)(2) (1) and [Pt(bpy)(hpip)](NO3)(2)center dot 2H(2)O (2) (bpy is 2,2'-bypyridine; pip is 2-phenylimidazo[4,5-f][1,10]phenanthroline; hpip is 2-(2-hydroxyphenyl) imidazo[4,5-f][1,10]phenanthroline), have been synthesized and fully characterized by CHN analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, H-1-NMR spectroscopy, IR spectroscopy (attenuated total reflection), and UV-vis spectroscopy. The DNA-binding behaviors of both complexes have been studied by spectroscopic methods and viscosity measurements, and their ability to inhibit DNA transcription was measured. The results indicate that both complexes show some degree of binding to DNA in an intercalative mode, resulting in intrinsic binding constants of (2.88 +/- A 0.4) x 10(4) and (5.38 +/- A 0.8) x 10(4) for 1 and 2, respectively. The comparatively observed difference in the DNA-binding affinities of the two complexes can be reasonably explained by the presence of intramolecular hydrogen bonding between the ortho phenolic group and the nitrogen atom of the imidazole ring. The extended coplanarity of the hpip ligand due to intramolecular hydrogen bonding may lead to an enhancement of the DNA-binding affinity of the hpip complex. In addition, the complexes can promote photocleavage of pUC19 DNA on irradiation as revealed by the spectroscopic and viscometric measurements, with 2 promoting cleavage of pUC19 DNA at lower concentration. Moreover, increasing concentrations of both complexes inhibited DNA transcription, and as expected 2 was shown to be a better antitumor agent than 1.
Açıklama
[dc.description]
WOS: 000316819300006
Açıklama
[dc.description]
PubMed: 23494397
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Pt(II) complex
Konu Başlıkları
[dc.subject]
Polypyridyl ligand
Konu Başlıkları
[dc.subject]
DNA binding
Konu Başlıkları
[dc.subject]
Photocleavage
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0949-8257
Sponsor YAYINCI
[dc.description.sponsorship]
Bulent Ecevit UniversityBulent Ecevit University [2011-03-13-06]
Sponsor YAYINCI
[dc.description.sponsorship]
We are grateful for the support of Bulent Ecevit University with grant 2011-03-13-06. We also thank Zehra Safi Oz for the use of her laboratory to make it possible to visualize and photograph the gel electrophoresis results.
İlk Sayfa Sayısı
[dc.identifier.startpage]
461
Son Sayfa Sayısı
[dc.identifier.endpage]
471
Dergi Adı
[dc.relation.journal]
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
18
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1007/s00775-013-0991-7
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3423
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
19
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
07 Şubat 2024 20:26
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Tıklayınız
complexes spectroscopy DNA-binding transcription spectroscopic bonding hydrogen binding measurements intramolecular 5-f][1 imidazole nitrogen phenolic between presence explained reasonably affinities difference observed extended cleavage antitumor better expected inhibited concentrations increasing Moreover concentration promoting coplanarity ligand enhancement
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