Effects of Nigella sativa on oxidative stress and beta-cell damage in streptozotocin-induced diabetic rats

The aim of the present study was to evaluate the possible protective effects of Nigella sativa L. (NS) against beta-cell damage from streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes. NS (0.2 ml/kg/day, i.p.) was injected for 3 days prior to STZ administration, and these injections were continued throughout the 4-week study. Oxidative stress is believed to play a role in the pathogenesis of diabetes mellitus (DM). To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in pancreatic homogenates. We also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Pancreatic beta-cells were examined by immunohistochemical methods. STZ induced a significant increase in lipid peroxidation and serum NO concentrations, and decreased antioxidant enzyme activity. NS treatment has been shown to provide a protective effect by decreasing lipid peroxidation and serum NO, and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining was observed in rats with STZ-induced diabetes. Increased intensity of staining for insulin, and preservation of beta-cell numbers were apparent in the NS-treated diabetic rats. These findings suggest that NS treatment exerts a therapeutic protective effect in diabetes by decreasing oxidative stress and preserving pancreatic beta-cell integrity. Consequently, NS may be clinically useful for protecting beta-cells against oxidative stress. (C) 2004 Wiley-Liss, Inc.

Dergi Adı ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
Dergi Cilt Bilgisi 279A
Dergi Sayısı 1
Sayfalar 685 - 691
Yayın Yılı 2004
Eser Adı
[dc.title]
Effects of Nigella sativa on oxidative stress and beta-cell damage in streptozotocin-induced diabetic rats
Yazar
[dc.contributor.author]
Kanter, M
Yazar
[dc.contributor.author]
Coskun, O
Yazar
[dc.contributor.author]
Korkmaz, A
Yazar
[dc.contributor.author]
Oter, S
Yayın Yılı
[dc.date.issued]
2004
Yayıncı
[dc.publisher]
WILEY-LISS
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
The aim of the present study was to evaluate the possible protective effects of Nigella sativa L. (NS) against beta-cell damage from streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes. NS (0.2 ml/kg/day, i.p.) was injected for 3 days prior to STZ administration, and these injections were continued throughout the 4-week study. Oxidative stress is believed to play a role in the pathogenesis of diabetes mellitus (DM). To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in pancreatic homogenates. We also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Pancreatic beta-cells were examined by immunohistochemical methods. STZ induced a significant increase in lipid peroxidation and serum NO concentrations, and decreased antioxidant enzyme activity. NS treatment has been shown to provide a protective effect by decreasing lipid peroxidation and serum NO, and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining was observed in rats with STZ-induced diabetes. Increased intensity of staining for insulin, and preservation of beta-cell numbers were apparent in the NS-treated diabetic rats. These findings suggest that NS treatment exerts a therapeutic protective effect in diabetes by decreasing oxidative stress and preserving pancreatic beta-cell integrity. Consequently, NS may be clinically useful for protecting beta-cells against oxidative stress. (C) 2004 Wiley-Liss, Inc.
Açıklama
[dc.description]
WOS: 000222466000012
Açıklama
[dc.description]
PubMed: 15224410
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Nigella sativa L.
Konu Başlıkları
[dc.subject]
diabetes mellitus
Konu Başlıkları
[dc.subject]
streptozotocin
Konu Başlıkları
[dc.subject]
oxidative stress
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1552-4884
ISSN
[dc.identifier.issn]
1552-4892
İlk Sayfa Sayısı
[dc.identifier.startpage]
685
Son Sayfa Sayısı
[dc.identifier.endpage]
691
Dergi Adı
[dc.relation.journal]
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
279A
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1002/ar.a.20056
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2498
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
22
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
06 Şubat 2024 19:37
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Tıklayınız
diabetes antioxidant pancreatic beta-cell peroxidation protective stress measured staining enzyme activity insulin treatment immunohistochemical decreasing against effect oxidative injected beta-cells examined provide determine whether oxidant imbalance between status decreased concentrations Pancreatic increase significant induced methods
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