Intravesical cationic nanoparticles of chitosan and polycaprolactone for the delivery of Mitomycin C to bladder tumors

Cationic nanoparticles of chitosan (CS), poly-epsilon-caprolactone coated with chitosan (CS-PCL) and poly-e-caprolactone coated with poly-L-lysine (PLL-PCL) were developed to encapsulate intravesical chemotherapeutic agent Mitomycin C (MMC) for longer residence time, higher local drug concentration and prevention of drug loss during bladder discharge. Nanoparticle diameters varied between 180 and 340 nm depending on polymer used for preparation and coating. Zeta potential values demonstrated positive charge expected from cationic nanoparticles. MMC encapsulation efficiency depended on hydrophilicity of polymers since MMC is water-soluble. Encapsulation was increased by 2-fold for CS-PCL and 3-fold for PLL-PCL as a consequence of hydrophilic coating. Complete drug release was obtained with only CS-PCL nanoparticles. On the other hand, CS and PLL-PCL nanoparticles did not completely liberate MMC due to strong polymer-drug interactions which were elucidated with DSC studies. As far as cellular interaction was concerned, CS-PCL was the most efficient formulation for uptake of fluorescent markers Nile Red and Rhodamine123 incorporated into nanoparticles. Especially, CS-PCL nanoparticles loaded with Rhodamine123 sharing hydrophilic properties with MMC were selectively incorporated by bladder cancer cell line, but not by normal bladder epithelial cells. CS-PCL nanoparticles seem to be promising for MMC delivery with respect to anticancer efficacy tested against MB49 bladder carcinoma cell line. (C) 2008 Elsevier B.V. All rights reserved.

Yazar Bilensoy, Erem
Sarisozen, Can
Esendagli, Guenes
Dogan, A. Lale
Aktas, Yesim
Sen, Murat
Mungan, N. Aydin
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2810
Tek Biçim Adres 10.1016/j.ijpharm.2008.12.015
Konu Başlıkları Mitomycin C
Chitosan
Poly-epsilon-caprolactone
Poly-L-lysine
Nanoparticle
Intravesical delivery
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı INTERNATIONAL JOURNAL OF PHARMACEUTICS
Dergi Cilt Bilgisi 371
Dergi Sayısı 01.Feb
Sayfalar 170 - 176
Yayın Yılı 2009
Eser Adı
[dc.title]
Intravesical cationic nanoparticles of chitosan and polycaprolactone for the delivery of Mitomycin C to bladder tumors
Yazar
[dc.contributor.author]
Bilensoy, Erem
Yazar
[dc.contributor.author]
Sarisozen, Can
Yazar
[dc.contributor.author]
Esendagli, Guenes
Yazar
[dc.contributor.author]
Dogan, A. Lale
Yazar
[dc.contributor.author]
Aktas, Yesim
Yazar
[dc.contributor.author]
Sen, Murat
Yazar
[dc.contributor.author]
Mungan, N. Aydin
Yayın Yılı
[dc.date.issued]
2009
Yayıncı
[dc.publisher]
ELSEVIER SCIENCE BV
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Cationic nanoparticles of chitosan (CS), poly-epsilon-caprolactone coated with chitosan (CS-PCL) and poly-e-caprolactone coated with poly-L-lysine (PLL-PCL) were developed to encapsulate intravesical chemotherapeutic agent Mitomycin C (MMC) for longer residence time, higher local drug concentration and prevention of drug loss during bladder discharge. Nanoparticle diameters varied between 180 and 340 nm depending on polymer used for preparation and coating. Zeta potential values demonstrated positive charge expected from cationic nanoparticles. MMC encapsulation efficiency depended on hydrophilicity of polymers since MMC is water-soluble. Encapsulation was increased by 2-fold for CS-PCL and 3-fold for PLL-PCL as a consequence of hydrophilic coating. Complete drug release was obtained with only CS-PCL nanoparticles. On the other hand, CS and PLL-PCL nanoparticles did not completely liberate MMC due to strong polymer-drug interactions which were elucidated with DSC studies. As far as cellular interaction was concerned, CS-PCL was the most efficient formulation for uptake of fluorescent markers Nile Red and Rhodamine123 incorporated into nanoparticles. Especially, CS-PCL nanoparticles loaded with Rhodamine123 sharing hydrophilic properties with MMC were selectively incorporated by bladder cancer cell line, but not by normal bladder epithelial cells. CS-PCL nanoparticles seem to be promising for MMC delivery with respect to anticancer efficacy tested against MB49 bladder carcinoma cell line. (C) 2008 Elsevier B.V. All rights reserved.
Açıklama
[dc.description]
WOS: 000265361300023
Açıklama
[dc.description]
PubMed: 19135514
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Mitomycin C
Konu Başlıkları
[dc.subject]
Chitosan
Konu Başlıkları
[dc.subject]
Poly-epsilon-caprolactone
Konu Başlıkları
[dc.subject]
Poly-L-lysine
Konu Başlıkları
[dc.subject]
Nanoparticle
Konu Başlıkları
[dc.subject]
Intravesical delivery
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0378-5173
ISSN
[dc.identifier.issn]
1873-3476
Sponsor YAYINCI
[dc.description.sponsorship]
Turkish Council of Scientific and Technical Research TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [107S247]
Sponsor YAYINCI
[dc.description.sponsorship]
Authors wish to acknowledge that this study was financially supported by Turkish Council of Scientific and Technical Research TUBITAK Scientific Research Project SBAG-HD-235 (107S247).
İlk Sayfa Sayısı
[dc.identifier.startpage]
170
Son Sayfa Sayısı
[dc.identifier.endpage]
176
Dergi Adı
[dc.relation.journal]
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Dergi Sayısı
[dc.identifier.issue]
01.Feb
Dergi Cilt Bilgisi
[dc.identifier.volume]
371
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.ijpharm.2008.12.015
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2810
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
16
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 06:57
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Tıklayınız
nanoparticles CS-PCL bladder hydrophilic chitosan coating coated PLL-PCL Rhodamine123 incorporated formulation efficient concerned interaction cellular Cationic strong completely liberate fluorescent polymer-drug interactions elucidated studies uptake loaded markers respect reserved rights Elsevier carcinoma against tested efficacy
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