Protective role of alpha-tocopherol and caffeic acid phenethyl ester on ischemia-reperfusion injury via nitric oxide and myeloperoxidase in rat kidneys

Background: The aim of this study was to determine the acute effects of antioxidant caffeic acid phenethyl ester (CAPE) and alpha-tocopherol (vitamin E) on nitric oxide (NO) production, neutrophil infiltration, and antioxidant enzyme activities on an in vivo model of renal ischemia-reperfusion injury. Methods: Rats were divided into five equal groups each consisting six rats: sham operation, ischemia, ischemia-reperfusion (I/R), I/R plus CAPE, and I/R plus vitamin E groups. CAPE or vitamin E was administered intraperitoneally before reperfusion. After experimental procedure, rats were sacrificed and both ipsilateral and contralateral kidneys were removed and prepared for NO concentrations, myeloperoxidase (MPO), catalase (CAT) and superoxide dismutase (SOD) activities. Results: Acute administration of vitamin E decreased NO concentrations in both ipsilateral and contralateral renal tissues compared to I/R group. SOD activity was increased in I/R and I/R + CAPE groups compared to sham operation group. The most prominent results were encountered in MPO activities, which did not change in contralateral kidneys in both ischemia and I/R groups. There was a significant decrease in ipsilateral MPO activity in ischemia group and a significant increase in I/R group compared to sham operation group. Pretreatment with intraperitoneal CAPE significantly diminished the tissue MPO activity indicating the prevention of the neutrophil sequestration into the kidney. Conclusion: There is a role for CAPE in attenuation in renal damage after I/R injury of the kidney, in part at least by inhibition of neutrophil sequestration. (C) 2003 Elsevier B.V. All rights reserved.

Yazar Gurel, A
Armutcu, F
Sahin, S
Sogut, S
Ozyurt, H
Gulec, M
Kutlu, NO
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3189
Tek Biçim Adres 10.1016/j.cccn.2003.09.013
Konu Başlıkları reperfusion injury
kidney
caffeic acid phenethyl ester
alpha-tocopherol
nitric oxide
antioxidant enzymes
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı CLINICA CHIMICA ACTA
Dergi Cilt Bilgisi 339
Dergi Sayısı 01.Feb
Sayfalar 33 - 41
Yayın Yılı 2004
Eser Adı
[dc.title]
Protective role of alpha-tocopherol and caffeic acid phenethyl ester on ischemia-reperfusion injury via nitric oxide and myeloperoxidase in rat kidneys
Yazar
[dc.contributor.author]
Gurel, A
Yazar
[dc.contributor.author]
Armutcu, F
Yazar
[dc.contributor.author]
Sahin, S
Yazar
[dc.contributor.author]
Sogut, S
Yazar
[dc.contributor.author]
Ozyurt, H
Yazar
[dc.contributor.author]
Gulec, M
Yazar
[dc.contributor.author]
Kutlu, NO
Yayın Yılı
[dc.date.issued]
2004
Yayıncı
[dc.publisher]
ELSEVIER SCIENCE BV
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background: The aim of this study was to determine the acute effects of antioxidant caffeic acid phenethyl ester (CAPE) and alpha-tocopherol (vitamin E) on nitric oxide (NO) production, neutrophil infiltration, and antioxidant enzyme activities on an in vivo model of renal ischemia-reperfusion injury. Methods: Rats were divided into five equal groups each consisting six rats: sham operation, ischemia, ischemia-reperfusion (I/R), I/R plus CAPE, and I/R plus vitamin E groups. CAPE or vitamin E was administered intraperitoneally before reperfusion. After experimental procedure, rats were sacrificed and both ipsilateral and contralateral kidneys were removed and prepared for NO concentrations, myeloperoxidase (MPO), catalase (CAT) and superoxide dismutase (SOD) activities. Results: Acute administration of vitamin E decreased NO concentrations in both ipsilateral and contralateral renal tissues compared to I/R group. SOD activity was increased in I/R and I/R + CAPE groups compared to sham operation group. The most prominent results were encountered in MPO activities, which did not change in contralateral kidneys in both ischemia and I/R groups. There was a significant decrease in ipsilateral MPO activity in ischemia group and a significant increase in I/R group compared to sham operation group. Pretreatment with intraperitoneal CAPE significantly diminished the tissue MPO activity indicating the prevention of the neutrophil sequestration into the kidney. Conclusion: There is a role for CAPE in attenuation in renal damage after I/R injury of the kidney, in part at least by inhibition of neutrophil sequestration. (C) 2003 Elsevier B.V. All rights reserved.
Açıklama
[dc.description]
WOS: 000188214900004
Açıklama
[dc.description]
PubMed: 14687891
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
reperfusion injury
Konu Başlıkları
[dc.subject]
kidney
Konu Başlıkları
[dc.subject]
caffeic acid phenethyl ester
Konu Başlıkları
[dc.subject]
alpha-tocopherol
Konu Başlıkları
[dc.subject]
nitric oxide
Konu Başlıkları
[dc.subject]
antioxidant enzymes
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0009-8981
ISSN
[dc.identifier.issn]
1873-3492
İlk Sayfa Sayısı
[dc.identifier.startpage]
33
Son Sayfa Sayısı
[dc.identifier.endpage]
41
Dergi Adı
[dc.relation.journal]
CLINICA CHIMICA ACTA
Dergi Sayısı
[dc.identifier.issue]
01.Feb
Dergi Cilt Bilgisi
[dc.identifier.volume]
339
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.cccn.2003.09.013
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3189
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
12
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
27 Aralık 2023 18:37
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Tıklayınız
groups ipsilateral ischemia activities operation neutrophil vitamin contralateral activity compared significant kidneys concentrations injury ischemia-reperfusion kidney sequestration antioxidant inhibition encountered results Elsevier prominent increased rights reserved tissues decrease tissue diminished prevention significantly administration intraperitoneal Pretreatment
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