The Effect of the Botulinum Toxin-A on Craniofacial Development An Experimental Study

In this study, we developed a novel experimental model to evaluate muscular action on bone formation and remodeling by the help of Botulinum toxin-A (BTX). Forty-nine 15-day-old mate Wistar rats were put into 4 groups randomly. Group I was the control group. BTX 0.4 IU (0.05 mL) was injected into the right masseter muscle in group 2 and into right temporalis muscle in group 3. The same volume of sterile saline was given into the both above-mentioned muscles in group 4. At the end of the fourth month, all animals were killed. Histology and weight of the masseter and temporalis muscles were studied. Thirty different osteometric measurements were also taken from skulls. Significant atrophy in BTX injected muscles was observed in groups 2 and 3. In group 4 (saline injection), only few osteometric measurements were significantly reduced, indicating the effect of the injection itself. Both groups 2 and 3 have apparent decrease in nasal bone, premaxilla, maxilla, and zygomatic dimensions on the injected side. When masseter group was compared with control and saline groups, no significant difference was found in skull base dimensions and mandibular length. In contrast, temporal group has also shown significant decrease in skull base dimensions. Our conclusions are as follows: (1) With this model, it is possible to study muscular action on bone formation and modeling without any surgical intervention, that is, by avoiding surgical artifacts, such as scar and contracture; (2) denervation of the skeletal muscles with BTX during the growing phase does effect bone development in a negative way; (3) pediatric use of the BTX deserves reevaluation under the light of these findings.

Yazar Babuccu, Berfu
Babuccu, Orhan
Yurdakan, Gamze
Ankarali, Handan
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3536
Tek Biçim Adres 10.1097/SAP.0b013e31818d4559
Konu Başlıkları botulinum toxin
craniofacial development
model
muscle action
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı ANNALS OF PLASTIC SURGERY
Dergi Cilt Bilgisi 63
Dergi Sayısı 4
Sayfalar 449 - 456
Yayın Yılı 2009
Eser Adı
[dc.title]
The Effect of the Botulinum Toxin-A on Craniofacial Development An Experimental Study
Yazar
[dc.contributor.author]
Babuccu, Berfu
Yazar
[dc.contributor.author]
Babuccu, Orhan
Yazar
[dc.contributor.author]
Yurdakan, Gamze
Yazar
[dc.contributor.author]
Ankarali, Handan
Yayın Yılı
[dc.date.issued]
2009
Yayıncı
[dc.publisher]
LIPPINCOTT WILLIAMS & WILKINS
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
In this study, we developed a novel experimental model to evaluate muscular action on bone formation and remodeling by the help of Botulinum toxin-A (BTX). Forty-nine 15-day-old mate Wistar rats were put into 4 groups randomly. Group I was the control group. BTX 0.4 IU (0.05 mL) was injected into the right masseter muscle in group 2 and into right temporalis muscle in group 3. The same volume of sterile saline was given into the both above-mentioned muscles in group 4. At the end of the fourth month, all animals were killed. Histology and weight of the masseter and temporalis muscles were studied. Thirty different osteometric measurements were also taken from skulls. Significant atrophy in BTX injected muscles was observed in groups 2 and 3. In group 4 (saline injection), only few osteometric measurements were significantly reduced, indicating the effect of the injection itself. Both groups 2 and 3 have apparent decrease in nasal bone, premaxilla, maxilla, and zygomatic dimensions on the injected side. When masseter group was compared with control and saline groups, no significant difference was found in skull base dimensions and mandibular length. In contrast, temporal group has also shown significant decrease in skull base dimensions. Our conclusions are as follows: (1) With this model, it is possible to study muscular action on bone formation and modeling without any surgical intervention, that is, by avoiding surgical artifacts, such as scar and contracture; (2) denervation of the skeletal muscles with BTX during the growing phase does effect bone development in a negative way; (3) pediatric use of the BTX deserves reevaluation under the light of these findings.
Açıklama
[dc.description]
WOS: 000270209000023
Açıklama
[dc.description]
PubMed: 19745716
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
botulinum toxin
Konu Başlıkları
[dc.subject]
craniofacial development
Konu Başlıkları
[dc.subject]
model
Konu Başlıkları
[dc.subject]
muscle action
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0148-7043
ISSN
[dc.identifier.issn]
1536-3708
İlk Sayfa Sayısı
[dc.identifier.startpage]
449
Son Sayfa Sayısı
[dc.identifier.endpage]
456
Dergi Adı
[dc.relation.journal]
ANNALS OF PLASTIC SURGERY
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
63
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1097/SAP.0b013e31818d4559
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3536
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
19
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
07 Şubat 2024 13:40
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Tıklayınız
groups muscles masseter injected dimensions muscle decrease saline temporalis surgical osteometric measurements significant effect control formation muscular action conclusions temporal reevaluation contrast length mandibular difference compared findings zygomatic maxilla follows denervation negative skeletal during contracture
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