Exercise training prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas

The aim of the present study was the evaluation of possible protective effects of exercise against beta-cell damage in streptozotocin (STZ)-induced diabetes in rats. The animals were divided into five groups: the control group, the STZ-induced diabetes group, the STZ-induced diabetes and light-intensity exercise group, the STZ-induced diabetes and moderate-intensity exercise group, and the STZ-induced diabetes and heavy-intensity exercise group. Animals in the exercise groups were made to swim one of three exercise protocols once a day for 12 consecutive weeks. STZ was injected intraperitoneally at a single dose of 50 mg/kg for diabetes induction. Exercise training was continued for 4 weeks prior to STZ administration; these applications were continued end of the study (for 12 weeks). Erythrocyte and pancreatic tissue malondialdehyde (MDA) levels and serum nitric oxide (NO) concentration were measured. Moreover glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) were also measured in pancreatic homogenates. Pancreatic beta-cells were examined by immunohistochemical methods. STZ increased lipid peroxidation and decreased the antioxidant enzyme activity significantly. Exercise, especially moderate-intensity exercise has shown protective effect probably through decreasing lipid peroxidation and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining were observed in STZ induced diabetic rats. Increased intensity of staining for insulin and preservation of beta-cell numbers were apparent in the exercise-applied diabetic rats. Interestingly, the best result was obtained from moderate-intensity exercise. These findings suggest that exercise has a therapeutic and/or protective effect in diabetes by decreasing oxidative stress and preservation of pancreatic beta-cell integrity. (C) 2004 Tohoku University Medical Press.

Yazar Coskun, O
Ocakci, A
Bayraktaroglu, T
Kanter, M
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2622
Tek Biçim Adres 10.1620/tjem.203.145
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
Dergi Cilt Bilgisi 203
Dergi Sayısı 3
Sayfalar 145 - 154
Yayın Yılı 2004
Eser Adı
[dc.title]
Exercise training prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas
Yazar
[dc.contributor.author]
Coskun, O
Yazar
[dc.contributor.author]
Ocakci, A
Yazar
[dc.contributor.author]
Bayraktaroglu, T
Yazar
[dc.contributor.author]
Kanter, M
Yayın Yılı
[dc.date.issued]
2004
Yayıncı
[dc.publisher]
TOHOKU UNIV MEDICAL PRESS
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
The aim of the present study was the evaluation of possible protective effects of exercise against beta-cell damage in streptozotocin (STZ)-induced diabetes in rats. The animals were divided into five groups: the control group, the STZ-induced diabetes group, the STZ-induced diabetes and light-intensity exercise group, the STZ-induced diabetes and moderate-intensity exercise group, and the STZ-induced diabetes and heavy-intensity exercise group. Animals in the exercise groups were made to swim one of three exercise protocols once a day for 12 consecutive weeks. STZ was injected intraperitoneally at a single dose of 50 mg/kg for diabetes induction. Exercise training was continued for 4 weeks prior to STZ administration; these applications were continued end of the study (for 12 weeks). Erythrocyte and pancreatic tissue malondialdehyde (MDA) levels and serum nitric oxide (NO) concentration were measured. Moreover glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) were also measured in pancreatic homogenates. Pancreatic beta-cells were examined by immunohistochemical methods. STZ increased lipid peroxidation and decreased the antioxidant enzyme activity significantly. Exercise, especially moderate-intensity exercise has shown protective effect probably through decreasing lipid peroxidation and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining were observed in STZ induced diabetic rats. Increased intensity of staining for insulin and preservation of beta-cell numbers were apparent in the exercise-applied diabetic rats. Interestingly, the best result was obtained from moderate-intensity exercise. These findings suggest that exercise has a therapeutic and/or protective effect in diabetes by decreasing oxidative stress and preservation of pancreatic beta-cell integrity. (C) 2004 Tohoku University Medical Press.
Açıklama
[dc.description]
WOS: 000222410000001
Açıklama
[dc.description]
PubMed: 15240923
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
0040-8727
ISSN
[dc.identifier.issn]
1349-3329
İlk Sayfa Sayısı
[dc.identifier.startpage]
145
Son Sayfa Sayısı
[dc.identifier.endpage]
154
Dergi Adı
[dc.relation.journal]
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
Dergi Sayısı
[dc.identifier.issue]
3
Dergi Cilt Bilgisi
[dc.identifier.volume]
203
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1620/tjem.203.145
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2622
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Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
8
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
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06 Şubat 2024 11:45
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Tıklayınız
exercise diabetes STZ-induced moderate-intensity pancreatic protective beta-cell continued preservation staining decreasing insulin effect diabetic activity measured Exercise peroxidation immunohistochemical antioxidant groups enzyme beta-cells examined Pancreatic increasing increased methods through probably decreased especially significantly degeneration numbers
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