DNA studies of newly synthesized heteroleptic platinum(II) complexes [Pt(bpy)(iip)](2+) and [Pt(bpy)(miip)](2+)

Two new mono-nuclear heteroleptic platinum(II) complexes, [Pt(bpy)(iip)](PF6)(2) (1) and [Pt(bpy)(miip)](PF6)(2)center dot 2H(2)O (2) (bpy is 2,2'-bipyridine; iip is 2-(imidazo-4-yl)-1H-imidazo[4,5-f] [1,10] phenanthroline; miip is 2-(1-methylimidazo-2-yl)-1H-imidazo[4,5-f] [1, 10] phenanthroline), have been synthesized and fully characterized by CHN analysis, electrospray ionization and MALDI-TOF mass spectrometry, H-1 NMR, FT-IR (ATR), and UV-Vis spectrophotometer. Cytotoxicity, ability to inhibit DNA transcription and DNAse activity of the complexes were studied. The DNA-binding behaviors of both complexes have also been studied by spectroscopic methods, cyclic voltammetry and viscosity measurements. Both complexes showed cytotoxic properties and 2 was more cytotoxic than 1. DNA transcription was inhibited upon increasing concentrations of both complexes. The complex 2 was found to be a better inhibitor than 1. The same pattern can be seen in the DNAse profile of the complexes. In addition, 2 was found to promote cleavage of pBR322 DNA at a lower concentration than 1. The spectroscopic, electrochemical and viscometric results indicate that both complexes show some degree of binding to DNA in an intercalative mode, resulting in intrinsic binding constants K (b) = 3.55 +/- A 0.6 x 10(4) M-1 and 7.01 +/- A 0.9 x 10(4) M-1 for 1 and 2, respectively. The difference in the DNA-binding affinities of 1 and 2 may presumably be explained by the methylated imidazole nitrogen atom that makes the compound more hydrophobic and gives better intercalative binding ability to DNA's hydrophobic environment.

Yazar Coban, Burak
Tekin, Ishak Ozel
Sengul, Abdurrahman
Yildiz, Ufuk
Kocak, Izzet
Sevinc, Nergis
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2405
Tek Biçim Adres 10.1007/s00775-015-1317-8
Konu Başlıkları Platinum
Polypyridyl ligand
DNA binding
Cytotoxicity
Transcription inhibition
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Dergi Cilt Bilgisi 21
Dergi Sayısı 2
Sayfalar 163 - 175
Yayın Yılı 2016
Eser Adı
[dc.title]
DNA studies of newly synthesized heteroleptic platinum(II) complexes [Pt(bpy)(iip)](2+) and [Pt(bpy)(miip)](2+)
Yazar
[dc.contributor.author]
Coban, Burak
Yazar
[dc.contributor.author]
Tekin, Ishak Ozel
Yazar
[dc.contributor.author]
Sengul, Abdurrahman
Yazar
[dc.contributor.author]
Yildiz, Ufuk
Yazar
[dc.contributor.author]
Kocak, Izzet
Yazar
[dc.contributor.author]
Sevinc, Nergis
Yayın Yılı
[dc.date.issued]
2016
Yayıncı
[dc.publisher]
SPRINGER
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Two new mono-nuclear heteroleptic platinum(II) complexes, [Pt(bpy)(iip)](PF6)(2) (1) and [Pt(bpy)(miip)](PF6)(2)center dot 2H(2)O (2) (bpy is 2,2'-bipyridine; iip is 2-(imidazo-4-yl)-1H-imidazo[4,5-f] [1,10] phenanthroline; miip is 2-(1-methylimidazo-2-yl)-1H-imidazo[4,5-f] [1, 10] phenanthroline), have been synthesized and fully characterized by CHN analysis, electrospray ionization and MALDI-TOF mass spectrometry, H-1 NMR, FT-IR (ATR), and UV-Vis spectrophotometer. Cytotoxicity, ability to inhibit DNA transcription and DNAse activity of the complexes were studied. The DNA-binding behaviors of both complexes have also been studied by spectroscopic methods, cyclic voltammetry and viscosity measurements. Both complexes showed cytotoxic properties and 2 was more cytotoxic than 1. DNA transcription was inhibited upon increasing concentrations of both complexes. The complex 2 was found to be a better inhibitor than 1. The same pattern can be seen in the DNAse profile of the complexes. In addition, 2 was found to promote cleavage of pBR322 DNA at a lower concentration than 1. The spectroscopic, electrochemical and viscometric results indicate that both complexes show some degree of binding to DNA in an intercalative mode, resulting in intrinsic binding constants K (b) = 3.55 +/- A 0.6 x 10(4) M-1 and 7.01 +/- A 0.9 x 10(4) M-1 for 1 and 2, respectively. The difference in the DNA-binding affinities of 1 and 2 may presumably be explained by the methylated imidazole nitrogen atom that makes the compound more hydrophobic and gives better intercalative binding ability to DNA's hydrophobic environment.
Açıklama
[dc.description]
WOS: 000373018700003
Açıklama
[dc.description]
PubMed: 26626200
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Platinum
Konu Başlıkları
[dc.subject]
Polypyridyl ligand
Konu Başlıkları
[dc.subject]
DNA binding
Konu Başlıkları
[dc.subject]
Cytotoxicity
Konu Başlıkları
[dc.subject]
Transcription inhibition
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0949-8257
ISSN
[dc.identifier.issn]
1432-1327
Sponsor YAYINCI
[dc.description.sponsorship]
TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113S165]
Sponsor YAYINCI
[dc.description.sponsorship]
We are grateful for the support of TUBITAK with Grant #113S165. We also thank Zehra Safi Oz for the use of her laboratory to make it possible to visualize and photograph the gel electrophoresis results.
İlk Sayfa Sayısı
[dc.identifier.startpage]
163
Son Sayfa Sayısı
[dc.identifier.endpage]
175
Dergi Adı
[dc.relation.journal]
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Dergi Sayısı
[dc.identifier.issue]
2
Dergi Cilt Bilgisi
[dc.identifier.volume]
21
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1007/s00775-015-1317-8
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2405
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
8
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
27 Aralık 2023 09:36
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Tıklayınız
complexes binding ability transcription spectroscopic intercalative studied cytotoxic better hydrophobic DNA-binding concentration promote addition indicate cleavage pBR322 viscometric electrochemical degree results affinities environment compound nitrogen imidazole methylated explained presumably difference resulting respectively constants intrinsic profile
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