The biochemical effectiveness of N-acetylcysteine in experimental spinal cord injury in rats

BACKGROUND In this study, we investigated the biochemical effectiveness of methylprednisolone, N-acetylcysteine (NAG) and methylprednisolone combined with NAC treatment in experimental spinal cord injury in rats. METHODS Thirty-two Sprague-Dawley male rats weighing 250-300 g were divided into four groups. Spinal cord injury was created extradurally with an aneurysm clip at the T4-T5 level. Following the trauma, Group C (Control group, n:8) was not given any treatment. Group M (methylprednisolone group, n:8) was treated with 30 mg.kg(-1) methylprednisolone followed by a maintenance dose of 5.4 mg.kg(-1) per hour. Group N (NAG group, n:8) was given 150 mg.kg(-1) NAG. Group MN (methylprednisolone and NAC group, n:8) was given 30 mg.kg(-1) followed by an hourly maintenance dose of 5.4 mg.kg(-1) methylprednisolone and 150 mg.kg(-1) NAC intraperitoneally. Twenty-four hours after the trauma, the rats were decapitated under anesthesia, and their spinal cord samples were taken for biochemical examination. RESULTS Mean malonyldialdehyde (MDA) values in Groups M, N and MN were significantly reduced compared to Group C. Mean superoxide dismutase (SOD) values in Groups M, N and MN were significantly higher than in Group C (p<0.05). No difference existed between Groups M and N with respect to mean MDA and SOD values. CONCLUSION Methylprednisolone, NAC and methylprednisolone plus NAC treatments have potential biochemical benefits in preventing secondary injury in experimental spinal cord injury in rats.

Yazar Hanci, Volkan
Kerimoglu, Alaeddin
Koca, Kenan
Baskesen, Aykut
Kilic, Kemal
Tastekin, Didem
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3461
Konu Başlıkları Experimental spinal cord injury
N-acetylcysteine
methylprednisolone
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı ULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY
Dergi Cilt Bilgisi 16
Dergi Sayısı 1
Sayfalar 15 - 21
Yayın Yılı 2010
Eser Adı
[dc.title]
The biochemical effectiveness of N-acetylcysteine in experimental spinal cord injury in rats
Yazar
[dc.contributor.author]
Hanci, Volkan
Yazar
[dc.contributor.author]
Kerimoglu, Alaeddin
Yazar
[dc.contributor.author]
Koca, Kenan
Yazar
[dc.contributor.author]
Baskesen, Aykut
Yazar
[dc.contributor.author]
Kilic, Kemal
Yazar
[dc.contributor.author]
Tastekin, Didem
Yayın Yılı
[dc.date.issued]
2010
Yayıncı
[dc.publisher]
TURKISH ASSOC TRAUMA EMERGENCY SURGERY
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
BACKGROUND In this study, we investigated the biochemical effectiveness of methylprednisolone, N-acetylcysteine (NAG) and methylprednisolone combined with NAC treatment in experimental spinal cord injury in rats. METHODS Thirty-two Sprague-Dawley male rats weighing 250-300 g were divided into four groups. Spinal cord injury was created extradurally with an aneurysm clip at the T4-T5 level. Following the trauma, Group C (Control group, n:8) was not given any treatment. Group M (methylprednisolone group, n:8) was treated with 30 mg.kg(-1) methylprednisolone followed by a maintenance dose of 5.4 mg.kg(-1) per hour. Group N (NAG group, n:8) was given 150 mg.kg(-1) NAG. Group MN (methylprednisolone and NAC group, n:8) was given 30 mg.kg(-1) followed by an hourly maintenance dose of 5.4 mg.kg(-1) methylprednisolone and 150 mg.kg(-1) NAC intraperitoneally. Twenty-four hours after the trauma, the rats were decapitated under anesthesia, and their spinal cord samples were taken for biochemical examination. RESULTS Mean malonyldialdehyde (MDA) values in Groups M, N and MN were significantly reduced compared to Group C. Mean superoxide dismutase (SOD) values in Groups M, N and MN were significantly higher than in Group C (p<0.05). No difference existed between Groups M and N with respect to mean MDA and SOD values. CONCLUSION Methylprednisolone, NAC and methylprednisolone plus NAC treatments have potential biochemical benefits in preventing secondary injury in experimental spinal cord injury in rats.
Açıklama
[dc.description]
WOS: 000273855400004
Açıklama
[dc.description]
PubMed: 20209390
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Experimental spinal cord injury
Konu Başlıkları
[dc.subject]
N-acetylcysteine
Konu Başlıkları
[dc.subject]
methylprednisolone
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1306-696X
İlk Sayfa Sayısı
[dc.identifier.startpage]
15
Son Sayfa Sayısı
[dc.identifier.endpage]
21
Dergi Adı
[dc.relation.journal]
ULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
16
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3461
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
16
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 07:00
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Tıklayınız
kg(-1) methylprednisolone injury biochemical Groups spinal values trauma significantly (methylprednisolone treatment maintenance experimental followed RESULTS samples examination anesthesia malonyldialdehyde reduced secondary preventing benefits potential treatments Methylprednisolone CONCLUSION respect compared between existed difference higher decapitated dismutase
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