Oxidized low-density-lipoprotein accumulation is associated with liver fibrosis in experimental cholestasis

OBJECTIVE: The aim of the present study was to examine the probable relationship between the accumulation of oxLDL and hepatic fibrogenesis in cholestatic rats. INTRODUCTION: There is growing evidence to support the current theories on how oxidative stress that results in lipid peroxidation is involved in the pathogenesis of cholestatic liver injury and fibrogenesis. One of the major and early lipid peroxidation products, OxLDL, is thought to play complex roles in various immuno-inflammatory mechanisms. METHODS: A prolonged (21-day) experimental bile duct ligation was performed on Wistar-albino rats. Biochemical analysis of blood, histopathologic evaluation of liver, measurement of the concentration of malondialdehyde (MDA) and superoxide-dismutase (SOD) in liver tissue homogenates, and immunofluorescent staining for oxLDL in liver tissue was conducted in bile-duct ligated (n = 8) and sham-operated rats (n = 8). RESULTS: Significantly higher levels of MDA and lower concentrations of SOD were detected in jaundiced rats than in the sham-operated rats. Positive oxLDL staining was also observed in liver tissue sections of jaundiced rats. Histopathological examination demonstrated that neither fibrosis nor other indications of hepatocellular injury were found in the sham-operated group, while features of severe hepatocellular injury, particularly fibrosis, were found in jaundiced rats. CONCLUSION: Our results support the finding that either oxLDLs are produced as an intermediate agent during exacerbated oxidative stress or they otherwise contribute to the various pathomechanisms underlying the process of liver fibrosis. Whatever the mechanism, it is clear that an association exists between elevated oxLDL levels and hepatocellular injury, particularly with fibrosis. Further studies are needed to evaluate the potential effects of oxLDLs on the progression of secondary biliary cirrhosis.

Yazar Karadeniz, Gueldeniz
Acikgoz, Serefden
Tekin, Ishak Ozel
Tascylar, Oge
Gun, Banu Dogan
Comert, Mustafa
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3060
Tek Biçim Adres 10.1590/S1807-59322008000400020
Konu Başlıkları cholestasis
liver fibrogenesis
lipid peroxidation
oxLDL
oxidative stress
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı CLINICS
Dergi Cilt Bilgisi 63
Dergi Sayısı 4
Sayfalar 531 - 540
Yayın Yılı 2008
Eser Adı
[dc.title]
Oxidized low-density-lipoprotein accumulation is associated with liver fibrosis in experimental cholestasis
Yazar
[dc.contributor.author]
Karadeniz, Gueldeniz
Yazar
[dc.contributor.author]
Acikgoz, Serefden
Yazar
[dc.contributor.author]
Tekin, Ishak Ozel
Yazar
[dc.contributor.author]
Tascylar, Oge
Yazar
[dc.contributor.author]
Gun, Banu Dogan
Yazar
[dc.contributor.author]
Comert, Mustafa
Yayın Yılı
[dc.date.issued]
2008
Yayıncı
[dc.publisher]
HOSPITAL CLINICAS, UNIV SAO PAULO
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
OBJECTIVE: The aim of the present study was to examine the probable relationship between the accumulation of oxLDL and hepatic fibrogenesis in cholestatic rats. INTRODUCTION: There is growing evidence to support the current theories on how oxidative stress that results in lipid peroxidation is involved in the pathogenesis of cholestatic liver injury and fibrogenesis. One of the major and early lipid peroxidation products, OxLDL, is thought to play complex roles in various immuno-inflammatory mechanisms. METHODS: A prolonged (21-day) experimental bile duct ligation was performed on Wistar-albino rats. Biochemical analysis of blood, histopathologic evaluation of liver, measurement of the concentration of malondialdehyde (MDA) and superoxide-dismutase (SOD) in liver tissue homogenates, and immunofluorescent staining for oxLDL in liver tissue was conducted in bile-duct ligated (n = 8) and sham-operated rats (n = 8). RESULTS: Significantly higher levels of MDA and lower concentrations of SOD were detected in jaundiced rats than in the sham-operated rats. Positive oxLDL staining was also observed in liver tissue sections of jaundiced rats. Histopathological examination demonstrated that neither fibrosis nor other indications of hepatocellular injury were found in the sham-operated group, while features of severe hepatocellular injury, particularly fibrosis, were found in jaundiced rats. CONCLUSION: Our results support the finding that either oxLDLs are produced as an intermediate agent during exacerbated oxidative stress or they otherwise contribute to the various pathomechanisms underlying the process of liver fibrosis. Whatever the mechanism, it is clear that an association exists between elevated oxLDL levels and hepatocellular injury, particularly with fibrosis. Further studies are needed to evaluate the potential effects of oxLDLs on the progression of secondary biliary cirrhosis.
Açıklama
[dc.description]
WOS: 000258504600020
Açıklama
[dc.description]
PubMed: 18719767
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
cholestasis
Konu Başlıkları
[dc.subject]
liver fibrogenesis
Konu Başlıkları
[dc.subject]
lipid peroxidation
Konu Başlıkları
[dc.subject]
oxLDL
Konu Başlıkları
[dc.subject]
oxidative stress
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
1807-5932
ISSN
[dc.identifier.issn]
1980-5322
İlk Sayfa Sayısı
[dc.identifier.startpage]
531
Son Sayfa Sayısı
[dc.identifier.endpage]
540
Dergi Adı
[dc.relation.journal]
CLINICS
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
63
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1590/S1807-59322008000400020
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3060
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
15
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
11 Nisan 2024 00:31
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Tıklayınız
injury fibrosis hepatocellular sham-operated tissue jaundiced particularly oxLDLs various peroxidation levels results staining stress support between fibrogenesis oxidative cholestatic association Histopathological studies indications needed neither demonstrated examination evaluate sections observed potential effects progression secondary Positive
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