Effects of whole-body hypoxic preconditioning on hypoxia/reoxygenation-induced intestinal injury in newborn rats

Purpose: The precise cause of necrotizing enterocolitis (NEC) is elusive. Ischemia and reperfusion injury of the intestine has been considered to be a major contributing factor for NEC. Ischemic preconditioning is defined as one or more brief periods of ischemia with intermittent reperfusion that protects tissues against a sustained period of subsequent ischemia. Contribution of preconditioning to hypoxia/reoxygenation-induced intestinal injury in newborn rats has not been evaluated previously. Methods: The study was carried out on 1-day-old Wistar albino rat pups. Whole-body hypoxia and reoxygenation (H/R) was achieved by 10 min hypoxia using 95% N-2 + 5% CO2 followed by 10 min reoxygenation with 100% oxygen. Whole body hypoxic preconditioning (HP) cycles were performed with 3 min hypoxia and 5 min reoxygenation. Thirty-three pups were randomly allocated into 4 groups. Group 1 served as untreated controls. The pups in group 2 were subjected to H/R only. In groups 3 and 4, 1 cycle and 3 cycles of HP were performed prior to H/R, respectively. Animals were killed at the end of the protocols. Intestine specimens were obtained to determine the histological changes, as well as to measure the tissue malondialdehyde (MDA) and nitric oxide (NO) levels, and xanthine oxidase (XO) and myeloperoxidase (MPO) activities. Results: The microscopic lesions in H/R rat pups were virtually the same as those seen in neonatal NEC, with severe destruction of villi and crypts, in some cases extending to the muscularis. In both HP groups, the lesions were found to be milder. H/R resulted in a marked elevation in MDA and NO levels, and XO and MPO activities compared to the untreated controls. Both 1 cycle and 3 cycles of HP prior to H/R resulted in an obvious decrease in all biochemical parameters. Differences of the biochemical results between both HP groups were not statistically significant. Conclusion: This study revealed that whole-body hypoxic preconditioning is beneficial for hypoxia/reoxygenation-induced intestinal injury in newborn rats.

Yazar Ceylan, H
Yuncu, M
Gurel, A
Armutcu, F
Gergerlioglu, HS
Bagci, C
Demiryurek, AT
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2519
Tek Biçim Adres 10.1055/s-2005-865820
Konu Başlıkları ischemia reperfusion injury
necrotizing enterocolitis
ischemic preconditioning
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı EUROPEAN JOURNAL OF PEDIATRIC SURGERY
Dergi Cilt Bilgisi 15
Dergi Sayısı 5
Sayfalar 325 - 332
Yayın Yılı 2005
Eser Adı
[dc.title]
Effects of whole-body hypoxic preconditioning on hypoxia/reoxygenation-induced intestinal injury in newborn rats
Yazar
[dc.contributor.author]
Ceylan, H
Yazar
[dc.contributor.author]
Yuncu, M
Yazar
[dc.contributor.author]
Gurel, A
Yazar
[dc.contributor.author]
Armutcu, F
Yazar
[dc.contributor.author]
Gergerlioglu, HS
Yazar
[dc.contributor.author]
Bagci, C
Yazar
[dc.contributor.author]
Demiryurek, AT
Yayın Yılı
[dc.date.issued]
2005
Yayıncı
[dc.publisher]
GEORG THIEME VERLAG KG
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Purpose: The precise cause of necrotizing enterocolitis (NEC) is elusive. Ischemia and reperfusion injury of the intestine has been considered to be a major contributing factor for NEC. Ischemic preconditioning is defined as one or more brief periods of ischemia with intermittent reperfusion that protects tissues against a sustained period of subsequent ischemia. Contribution of preconditioning to hypoxia/reoxygenation-induced intestinal injury in newborn rats has not been evaluated previously. Methods: The study was carried out on 1-day-old Wistar albino rat pups. Whole-body hypoxia and reoxygenation (H/R) was achieved by 10 min hypoxia using 95% N-2 + 5% CO2 followed by 10 min reoxygenation with 100% oxygen. Whole body hypoxic preconditioning (HP) cycles were performed with 3 min hypoxia and 5 min reoxygenation. Thirty-three pups were randomly allocated into 4 groups. Group 1 served as untreated controls. The pups in group 2 were subjected to H/R only. In groups 3 and 4, 1 cycle and 3 cycles of HP were performed prior to H/R, respectively. Animals were killed at the end of the protocols. Intestine specimens were obtained to determine the histological changes, as well as to measure the tissue malondialdehyde (MDA) and nitric oxide (NO) levels, and xanthine oxidase (XO) and myeloperoxidase (MPO) activities. Results: The microscopic lesions in H/R rat pups were virtually the same as those seen in neonatal NEC, with severe destruction of villi and crypts, in some cases extending to the muscularis. In both HP groups, the lesions were found to be milder. H/R resulted in a marked elevation in MDA and NO levels, and XO and MPO activities compared to the untreated controls. Both 1 cycle and 3 cycles of HP prior to H/R resulted in an obvious decrease in all biochemical parameters. Differences of the biochemical results between both HP groups were not statistically significant. Conclusion: This study revealed that whole-body hypoxic preconditioning is beneficial for hypoxia/reoxygenation-induced intestinal injury in newborn rats.
Açıklama
[dc.description]
WOS: 000233110500004
Açıklama
[dc.description]
PubMed: 16254844
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
ischemia reperfusion injury
Konu Başlıkları
[dc.subject]
necrotizing enterocolitis
Konu Başlıkları
[dc.subject]
ischemic preconditioning
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0939-7248
ISSN
[dc.identifier.issn]
1439-359X
İlk Sayfa Sayısı
[dc.identifier.startpage]
325
Son Sayfa Sayısı
[dc.identifier.endpage]
332
Dergi Adı
[dc.relation.journal]
EUROPEAN JOURNAL OF PEDIATRIC SURGERY
Dergi Sayısı
[dc.identifier.issue]
5
Dergi Cilt Bilgisi
[dc.identifier.volume]
15
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1055/s-2005-865820
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2519
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
7
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
27 Ocak 2024 23:35
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Tıklayınız
groups preconditioning reoxygenation cycles hypoxia injury hypoxic hypoxia/reoxygenation-induced activities intestinal newborn levels performed resulted reperfusion biochemical controls untreated ischemia lesions specimens obtained determine Results nitric histological myeloperoxidase measure oxidase xanthine changes malondialdehyde tissue microscopic Purpose
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