The evaluation of cystatin C, IL-1 beta, and TNF-alpha levels in total saliva and gingival crevicular fluid from 11- to 16-year-old children

Background: The aim of this study was to evaluate the levels of cystatin C, interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) in the total saliva and gingival crevicular fluid (GCF) of periodontally healthy children (PHC) and children with gingivitis (CG) who were between I I and 16 years old. Methods: The study was carried out with 10 PHC and 25 CG. Unstimulated total saliva and GCF samples were obtained. Clinical parameters, including probing depth (PD), clinical attachment loss (CAL), plaque index (PI), gingival index (GI), and gingival bleeding index (GBI), were assessed. GCF samples were collected from four maxillary upper incisors. After sampling, biochemical analyses were performed using latex particle-enhanced turbidimetric immunoassay for cystatin C and enzyme-linked immunosorbent assay for IL-1 beta and TNF-alpha. The multivariate analysis of variance test was used for statistical evaluation. Results: In total saliva, cystatin C and TNF-a levels were higher in PHC, and IL-1 beta levels were higher in CG, but the differences were not statistically significant. In GCF, cystatin C levels were higher in PHC (P >0.05), whereas TNF-alpha and IL-1 beta levels were higher in CG (P >0.05). In the CG group, there were positive correlations between the GCF cystatin C level and the PI of the sampled site (r = 0.488; P <0.05); also, GCF IL-1 beta (r = 0.603; P <0.05) and TNF-a (r = 0.456; P <0.05) levels were positively correlated with PD and CAL. For the whole mouth and the sampled sites, PI, GI, GBI, PD, and CAL values were higher in CG (P<0.05), but no significant differences were detected between GCF volumes of the two groups. Conclusions: To the best of our knowledge, this study represents the first evaluation of cystatin C in the gingival disease mechanism in children. Our results showed that total saliva and GCF cystatin C levels were higher in PHC (P >0.05), but there was no correlation between cystatin C levels and IL-1 beta or TNF-alpha levels in total saliva or GCF.

Yazar Ulker, A. Evren
Tulunoglu, Oezlem
Ozmeric, Nurdan
Can, Murat
Demirtas, Selda
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3579
Tek Biçim Adres 10.1902/jop.2008.070422
Konu Başlıkları children
cystatin C
gingival crevicular fluid
interleukin-1 beta
saliva
tumor necrosis factor-alpha
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı JOURNAL OF PERIODONTOLOGY
Dergi Cilt Bilgisi 79
Dergi Sayısı 5
Sayfalar 854 - 860
Yayın Yılı 2008
Eser Adı
[dc.title]
The evaluation of cystatin C, IL-1 beta, and TNF-alpha levels in total saliva and gingival crevicular fluid from 11- to 16-year-old children
Yazar
[dc.contributor.author]
Ulker, A. Evren
Yazar
[dc.contributor.author]
Tulunoglu, Oezlem
Yazar
[dc.contributor.author]
Ozmeric, Nurdan
Yazar
[dc.contributor.author]
Can, Murat
Yazar
[dc.contributor.author]
Demirtas, Selda
Yayın Yılı
[dc.date.issued]
2008
Yayıncı
[dc.publisher]
AMER ACAD PERIODONTOLOGY
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background: The aim of this study was to evaluate the levels of cystatin C, interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) in the total saliva and gingival crevicular fluid (GCF) of periodontally healthy children (PHC) and children with gingivitis (CG) who were between I I and 16 years old. Methods: The study was carried out with 10 PHC and 25 CG. Unstimulated total saliva and GCF samples were obtained. Clinical parameters, including probing depth (PD), clinical attachment loss (CAL), plaque index (PI), gingival index (GI), and gingival bleeding index (GBI), were assessed. GCF samples were collected from four maxillary upper incisors. After sampling, biochemical analyses were performed using latex particle-enhanced turbidimetric immunoassay for cystatin C and enzyme-linked immunosorbent assay for IL-1 beta and TNF-alpha. The multivariate analysis of variance test was used for statistical evaluation. Results: In total saliva, cystatin C and TNF-a levels were higher in PHC, and IL-1 beta levels were higher in CG, but the differences were not statistically significant. In GCF, cystatin C levels were higher in PHC (P >0.05), whereas TNF-alpha and IL-1 beta levels were higher in CG (P >0.05). In the CG group, there were positive correlations between the GCF cystatin C level and the PI of the sampled site (r = 0.488; P <0.05); also, GCF IL-1 beta (r = 0.603; P <0.05) and TNF-a (r = 0.456; P <0.05) levels were positively correlated with PD and CAL. For the whole mouth and the sampled sites, PI, GI, GBI, PD, and CAL values were higher in CG (P<0.05), but no significant differences were detected between GCF volumes of the two groups. Conclusions: To the best of our knowledge, this study represents the first evaluation of cystatin C in the gingival disease mechanism in children. Our results showed that total saliva and GCF cystatin C levels were higher in PHC (P >0.05), but there was no correlation between cystatin C levels and IL-1 beta or TNF-alpha levels in total saliva or GCF.
Açıklama
[dc.description]
WOS: 000255961500012
Açıklama
[dc.description]
PubMed: 18454664
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
children
Konu Başlıkları
[dc.subject]
cystatin C
Konu Başlıkları
[dc.subject]
gingival crevicular fluid
Konu Başlıkları
[dc.subject]
interleukin-1 beta
Konu Başlıkları
[dc.subject]
saliva
Konu Başlıkları
[dc.subject]
tumor necrosis factor-alpha
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0022-3492
ISSN
[dc.identifier.issn]
1943-3670
İlk Sayfa Sayısı
[dc.identifier.startpage]
854
Son Sayfa Sayısı
[dc.identifier.endpage]
860
Dergi Adı
[dc.relation.journal]
JOURNAL OF PERIODONTOLOGY
Dergi Sayısı
[dc.identifier.issue]
5
Dergi Cilt Bilgisi
[dc.identifier.volume]
79
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1902/jop.2008.070422
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3579
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
9
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 22:12
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Tıklayınız
levels cystatin higher saliva gingival between children TNF-alpha sampled differences samples evaluation significant correlations statistical positive whereas Results statistically Background groups correlation showed results mechanism disease represents knowledge Conclusions volumes detected values correlated positively performed
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