In vitro evaluation of antisense oligonucleotide functionalized core-shell nanoparticles loaded with -tocopherol succinate

Antisense oligonucleotide (ASO)-conjugated--tocopherol succinate (TCS)-loaded-poly(lactic acid)-g-poly(ethylene glycol) nanoparticles (ASO-TCS-PLA-PEG NPs), with the ratio of polymer/TCS of 10:2.5, 10:5, 10:7 (w/w) were prepared for targeting cancer therapy. The amphiphilic PLA, amino terminated PEG graft copolymers were synthesized by ring opening polymerization reaction. Nanoparticles were produced by using double emulsion (w/o/w) solvent evaporation method. ASO-TCS-PLA-PEG NPs demonstrated satisfactory encapsulation and loading efficiency and size distribution. The short-term stability studies were carried out at 4 and 25 degrees C for 30days to assess their mean particle size, polydispersity index and zeta potential. The cellular uptake and extended cytoplasmic retention of the NPs in A549 human lung carcinoma and L929 mouse fibroblast cells were examined by fluorescence and confocal microscopy. In human lung cancer cells, ASO-TCS-PLA-PEG NPs exhibited better cellular internalization, cytotoxicity and apoptotic and necrotic effects compared to healthy cell line, L929. These findings showed that ASO-modified nanoparticles could serve as a promising nanocarrier for targeted tumor cells.

Eser Adı
[dc.title]
In vitro evaluation of antisense oligonucleotide functionalized core-shell nanoparticles loaded with -tocopherol succinate
Yazar
[dc.contributor.author]
Kılıçay, Ebru
Yazar
[dc.contributor.author]
Karahaliloğlu, Zeynep
Yazar
[dc.contributor.author]
Alpaslan, Pınar
Yazar
[dc.contributor.author]
Hazer, Baki
Yazar
[dc.contributor.author]
Denkbaş, Emir Baki
Yayın Yılı
[dc.date.issued]
2017
Yayıncı
[dc.publisher]
TAYLOR & FRANCIS LTD
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Antisense oligonucleotide (ASO)-conjugated--tocopherol succinate (TCS)-loaded-poly(lactic acid)-g-poly(ethylene glycol) nanoparticles (ASO-TCS-PLA-PEG NPs), with the ratio of polymer/TCS of 10:2.5, 10:5, 10:7 (w/w) were prepared for targeting cancer therapy. The amphiphilic PLA, amino terminated PEG graft copolymers were synthesized by ring opening polymerization reaction. Nanoparticles were produced by using double emulsion (w/o/w) solvent evaporation method. ASO-TCS-PLA-PEG NPs demonstrated satisfactory encapsulation and loading efficiency and size distribution. The short-term stability studies were carried out at 4 and 25 degrees C for 30days to assess their mean particle size, polydispersity index and zeta potential. The cellular uptake and extended cytoplasmic retention of the NPs in A549 human lung carcinoma and L929 mouse fibroblast cells were examined by fluorescence and confocal microscopy. In human lung cancer cells, ASO-TCS-PLA-PEG NPs exhibited better cellular internalization, cytotoxicity and apoptotic and necrotic effects compared to healthy cell line, L929. These findings showed that ASO-modified nanoparticles could serve as a promising nanocarrier for targeted tumor cells.
Açıklama
[dc.description]
WOS: 000407503900010
Açıklama
[dc.description]
PubMed: 28696185
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
PLA-PEG
Konu Başlıkları
[dc.subject]
antisense oligonucleotide
Konu Başlıkları
[dc.subject]
-tocopherol succinate
Konu Başlıkları
[dc.subject]
drug delivery
Konu Başlıkları
[dc.subject]
human lung cancer cells
Künye
[dc.identifier.citation]
Kilicay, E., Karahaliloglu, Z., Alpaslan, P., Hazer, B. ve Denkbas, E. B. (2017). In vitro evaluation of antisense oligonucleotide functionalized core-shell nanoparticles loaded with -tocopherol succinate. Journal of Biomaterials Science-polymer Edition, 28(15), 1762–1785. doi:10.1080/09205063.2017.1354670
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0920-5063
ISSN
[dc.identifier.issn]
1568-5624
Sponsor YAYINCI
[dc.description.sponsorship]
Bulent Ecevit UniversityBulent Ecevit University [BEU-2017-YKD-33496813-01]
Sponsor YAYINCI
[dc.description.sponsorship]
This work was financially supported by Bulent Ecevit University [BEU-2017-YKD-33496813-01].
İlk Sayfa Sayısı
[dc.identifier.startpage]
1762
Son Sayfa Sayısı
[dc.identifier.endpage]
1785
Dergi Adı
[dc.relation.journal]
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Dergi Sayısı
[dc.identifier.issue]
15
Dergi Cilt Bilgisi
[dc.identifier.volume]
28
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1080/09205063.2017.1354670
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2763
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
17
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 06:27
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Tıklayınız
ASO-TCS-PLA-PEG cancer nanoparticles cellular fibroblast assess carcinoma particle uptake polydispersity retention potential cytoplasmic examined extended Antisense fluorescence targeted nanocarrier promising ASO-modified showed findings confocal healthy compared effects necrotic apoptotic cytotoxicity internalization 30days exhibited microscopy better
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