The effects of nitric oxide on the expression of cell adhesion molecules (ICAM-1, UEA-1, and tenascin) in rats with unilateral testicular torsion

Background/Purpose: The aim of this study was to determine the effects of nitric oxide (NO) on the expression of adhesion molecules in the early course of testicular I-R injury in rats. Methods: Forty male Sprague-Dawley rats were separated into 4 groups, each containing 10 rats. A sham operation was performed in group 1 (control). In group 2 (I-R), after 6 hours of unilateral testicular torsion, 1 -hour detorsion of the testis was performed. In group 3 (I-R/L-NAME), after performing the same surgical procedures as in group 11, L-NAME was given for 30 minutes. In group 4 (I-R/Mol), after performing the same surgical procedure (torsion and detorsion) as in group 11, molsidomine, an NO donor, was infused for 30 minutes. Then, ipsilateral orchiectomies were performed to measure the tissue levels of malondialdehyde (MDA) and NO and to make histologic examination. Results: MDA values and the testicular injury score decreased and NO values increased in the I-R/Mol-treated group compared with other experimental groups. The tenascin expression in the interstitial space and basement membrane of the tubuli seminiferi were milder in the I-R/Mol group compared with that of the I-R and the I-R/L-NAME. The acrosomes of the spermatids in I-R and I-R/L-NAME groups were stained mildly by lectin. In the I-R and I-R/L-NAME groups, the interstitial spaces, basement membrane of the tubuli seminiferi, and sertoli and germinal cells in testicular tissue were stained intensely by ICAM-1. Conclusions: The expression of adhesion molecules such as tenascin, lectin, and ICAM-1 in the totted testicular tissue may be a pathophysiologic sign of inflammation. NO regulates adhesion molecules expression.

Yazar Ozturk, H
Buyukbayram, H
Ozdemir, E
Ketani, A
Gurel, A
Onen, A
Otcu, S
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3565
Tek Biçim Adres 10.1016/S0022-3468(03)00573-6
Konu Başlıkları testicular torsion
nitric oxide
L-NAME
adhesion molecules
rat
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı JOURNAL OF PEDIATRIC SURGERY
Dergi Cilt Bilgisi 38
Dergi Sayısı 11
Sayfalar 1621 - 1627
Yayın Yılı 2003
Eser Adı
[dc.title]
The effects of nitric oxide on the expression of cell adhesion molecules (ICAM-1, UEA-1, and tenascin) in rats with unilateral testicular torsion
Yazar
[dc.contributor.author]
Ozturk, H
Yazar
[dc.contributor.author]
Buyukbayram, H
Yazar
[dc.contributor.author]
Ozdemir, E
Yazar
[dc.contributor.author]
Ketani, A
Yazar
[dc.contributor.author]
Gurel, A
Yazar
[dc.contributor.author]
Onen, A
Yazar
[dc.contributor.author]
Otcu, S
Yayın Yılı
[dc.date.issued]
2003
Yayıncı
[dc.publisher]
W B SAUNDERS CO
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background/Purpose: The aim of this study was to determine the effects of nitric oxide (NO) on the expression of adhesion molecules in the early course of testicular I-R injury in rats. Methods: Forty male Sprague-Dawley rats were separated into 4 groups, each containing 10 rats. A sham operation was performed in group 1 (control). In group 2 (I-R), after 6 hours of unilateral testicular torsion, 1 -hour detorsion of the testis was performed. In group 3 (I-R/L-NAME), after performing the same surgical procedures as in group 11, L-NAME was given for 30 minutes. In group 4 (I-R/Mol), after performing the same surgical procedure (torsion and detorsion) as in group 11, molsidomine, an NO donor, was infused for 30 minutes. Then, ipsilateral orchiectomies were performed to measure the tissue levels of malondialdehyde (MDA) and NO and to make histologic examination. Results: MDA values and the testicular injury score decreased and NO values increased in the I-R/Mol-treated group compared with other experimental groups. The tenascin expression in the interstitial space and basement membrane of the tubuli seminiferi were milder in the I-R/Mol group compared with that of the I-R and the I-R/L-NAME. The acrosomes of the spermatids in I-R and I-R/L-NAME groups were stained mildly by lectin. In the I-R and I-R/L-NAME groups, the interstitial spaces, basement membrane of the tubuli seminiferi, and sertoli and germinal cells in testicular tissue were stained intensely by ICAM-1. Conclusions: The expression of adhesion molecules such as tenascin, lectin, and ICAM-1 in the totted testicular tissue may be a pathophysiologic sign of inflammation. NO regulates adhesion molecules expression.
Açıklama
[dc.description]
WOS: 000186565200012
Açıklama
[dc.description]
PubMed: 14614712
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
testicular torsion
Konu Başlıkları
[dc.subject]
nitric oxide
Konu Başlıkları
[dc.subject]
L-NAME
Konu Başlıkları
[dc.subject]
adhesion molecules
Konu Başlıkları
[dc.subject]
rat
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0022-3468
İlk Sayfa Sayısı
[dc.identifier.startpage]
1621
Son Sayfa Sayısı
[dc.identifier.endpage]
1627
Dergi Adı
[dc.relation.journal]
JOURNAL OF PEDIATRIC SURGERY
Dergi Sayısı
[dc.identifier.issue]
11
Dergi Cilt Bilgisi
[dc.identifier.volume]
38
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/S0022-3468(03)00573-6
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3565
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
15
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
15 Nisan 2024 04:53
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Tıklayınız
testicular expression groups performed I-R/L-NAME tissue molecules adhesion tubuli values minutes seminiferi compared membrane surgical basement interstitial tenascin performing stained ICAM-1 injury lectin acrosomes inflammation pathophysiologic increased decreased regulates Results examination histologic malondialdehyde I-R/Mol-treated mildly
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