Results of a Multicenter Study Investigating Plasmid Mediated Colistin Resistance Genes (mcr-1 and mcr-2) in Clinical Enterobacteriaceae Isolates from Turkey

Colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmid-mediated colistin resistance gene mcr-2 in 2016. The rapid and interspecies dissemination of plasmid-mediated resistance mechanisms through horizontal gene transfer, have made these genes considerably threatening. After the first reports, although mcr-1/mcr-2 producing Enterobacteriaceae isolates have been reported from many countries, there have been no reports from Turkey. Thus, the aim of this study was to investigate the presence of mcr-1/mcr-2 in clinical Enterobacteriaceae isolates from different parts of our country. A total of 329 Enterobacteriaceae isolates from 22 laboratories were collected which were isolated between March, 2015 and February, 2016. mcr-1/mcr-2 were investigated by polymerase chain reaction during February-March, 2016. Two hundred and seventeen of Klebsiella pneumoniae (66%), 75 of Salmonella spp. (22.8%), 31 of Esherichia coli (9.4%), 3 of Enterobacter cloacae (0.9%), 2 of Klebsiella oxytoca (0.6%) and 1 of Enterobacter aerogenes (0.3%) isolates were included to the study. Agarose gel electrophoresis results of PCR studies have shown expected band sizes for positive control isolates as 309 bp for mcr-1 and 567 bp for mcr-2. However, the presence of mcr-1/mcr-2 genes was not detected among the tested study isolates of Enterobacteriaceae. Although mcr-1/mcr-2 were not detected in our study isolates, it is highly important to understand the mechanism of resistance dissemination and determine the resistant isolates by considering that colistin is a last-line antibiotic against infections of multidrug or carbapenem resistant gram-negative bacteria. Thus, it is suggested that these mechanisms should be followed-up in both clinical and non-clinical (e.g. isolates from food animals, raw meats and environment) isolates of special populations.

Yazar Sari, Ayse Nur
Suzuk, Serap
Karatuna, Onur
Ogunc, Dilara
Karakoc, Ayse Esra
Cizmeci, Zeynep
Aliskan, Hikmet Eda
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3266
Tek Biçim Adres 10.5578/mb.57515
Konu Başlıkları Colistin
mcr-1
mcr-2
Enterobacteriaceae
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı MIKROBIYOLOJI BULTENI
Dergi Cilt Bilgisi 51
Dergi Sayısı 3
Sayfalar 299 - 303
Yayın Yılı 2017
Eser Adı
[dc.title]
Results of a Multicenter Study Investigating Plasmid Mediated Colistin Resistance Genes (mcr-1 and mcr-2) in Clinical Enterobacteriaceae Isolates from Turkey
Yazar
[dc.contributor.author]
Sari, Ayse Nur
Yazar
[dc.contributor.author]
Suzuk, Serap
Yazar
[dc.contributor.author]
Karatuna, Onur
Yazar
[dc.contributor.author]
Ogunc, Dilara
Yazar
[dc.contributor.author]
Karakoc, Ayse Esra
Yazar
[dc.contributor.author]
Cizmeci, Zeynep
Yazar
[dc.contributor.author]
Aliskan, Hikmet Eda
Yayın Yılı
[dc.date.issued]
2017
Yayıncı
[dc.publisher]
ANKARA MICROBIOLOGY SOC
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmid-mediated colistin resistance gene mcr-2 in 2016. The rapid and interspecies dissemination of plasmid-mediated resistance mechanisms through horizontal gene transfer, have made these genes considerably threatening. After the first reports, although mcr-1/mcr-2 producing Enterobacteriaceae isolates have been reported from many countries, there have been no reports from Turkey. Thus, the aim of this study was to investigate the presence of mcr-1/mcr-2 in clinical Enterobacteriaceae isolates from different parts of our country. A total of 329 Enterobacteriaceae isolates from 22 laboratories were collected which were isolated between March, 2015 and February, 2016. mcr-1/mcr-2 were investigated by polymerase chain reaction during February-March, 2016. Two hundred and seventeen of Klebsiella pneumoniae (66%), 75 of Salmonella spp. (22.8%), 31 of Esherichia coli (9.4%), 3 of Enterobacter cloacae (0.9%), 2 of Klebsiella oxytoca (0.6%) and 1 of Enterobacter aerogenes (0.3%) isolates were included to the study. Agarose gel electrophoresis results of PCR studies have shown expected band sizes for positive control isolates as 309 bp for mcr-1 and 567 bp for mcr-2. However, the presence of mcr-1/mcr-2 genes was not detected among the tested study isolates of Enterobacteriaceae. Although mcr-1/mcr-2 were not detected in our study isolates, it is highly important to understand the mechanism of resistance dissemination and determine the resistant isolates by considering that colistin is a last-line antibiotic against infections of multidrug or carbapenem resistant gram-negative bacteria. Thus, it is suggested that these mechanisms should be followed-up in both clinical and non-clinical (e.g. isolates from food animals, raw meats and environment) isolates of special populations.
Açıklama
[dc.description]
WOS: 000408311400011
Açıklama
[dc.description]
PubMed: 28929967
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
tur
Konu Başlıkları
[dc.subject]
Colistin
Konu Başlıkları
[dc.subject]
mcr-1
Konu Başlıkları
[dc.subject]
mcr-2
Konu Başlıkları
[dc.subject]
Enterobacteriaceae
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0374-9096
İlk Sayfa Sayısı
[dc.identifier.startpage]
299
Son Sayfa Sayısı
[dc.identifier.endpage]
303
Dergi Adı
[dc.relation.journal]
MIKROBIYOLOJI BULTENI
Dergi Sayısı
[dc.identifier.issue]
3
Dergi Cilt Bilgisi
[dc.identifier.volume]
51
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.5578/mb.57515
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3266
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
27
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 22:24
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Tıklayınız
isolates resistance mcr-1/mcr-2 resistant Enterobacteriaceae Klebsiella plasmid-mediated colistin reports detected pneumoniae presence Enterobacter dissemination mechanisms Colistin clinical against infections multidrug carbapenem gram-negative antibiotic expected studies special results electrophoresis Agarose included aerogenes oxytoca cloacae Esherichia populations
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