Effects of L-arginine and NG-nitro L-arginine methyl ester on lipid peroxide, superoxide dismutase and nitrate levels after experimental sciatic nerve ischemia-reperfusion in rats

Nitric oxide (NO) has been reported to function in both cytoprotective and cytotoxic tissue ischemia-reperfusion (I/R). In this study, we evaluated the effects of L-arginine, the substrate for NO, and N-G-nitro L-argine methyl ester (L-NAME), NO synthase (NOS) inhibitor on superoxide dismutase (SOD) enzyme activity, malondialdehyde (MDA), a marker of lipid peroxidation, nitrate levels, and histopathological structure in rat sciatic nerve 2 h after ischemia, followed by 3 h of reperfusion. Reperfusion resulted in a significant increase in lipid peroxidation level and a decrease in nitrate level of the sciatic nerve. The increased level of lipid peroxidation was partly reduced by NOS inhibition. The decrease in sciatic nerve SOD level, observed in group subjected to I/R, was prevented by inhibition of NOS by L-NAME. These results were supported by histological findings that in the L-arginine-treated group, degenerations of both myelin sheath and axon were observed, while in the L-NAME-treated group, no pathological changes were detected. Our results suggested that excessive NO formation accelerates lipid peroxidation, as well as axonal degeneration on the early reperfusion period of the sciatic nerve.

Yazar Sayan, H
Ugurlu, B
Babul, A
Take, G
Erdogan, D
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2496
Tek Biçim Adres 10.1080/00207450490270578
Konu Başlıkları ischemia
nitric oxide
oxidative stress
reperfusion
sciatic nerve
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı INTERNATIONAL JOURNAL OF NEUROSCIENCE
Dergi Cilt Bilgisi 114
Dergi Sayısı 3
Sayfalar 349 - 364
Yayın Yılı 2004
Eser Adı
[dc.title]
Effects of L-arginine and NG-nitro L-arginine methyl ester on lipid peroxide, superoxide dismutase and nitrate levels after experimental sciatic nerve ischemia-reperfusion in rats
Yazar
[dc.contributor.author]
Sayan, H
Yazar
[dc.contributor.author]
Ugurlu, B
Yazar
[dc.contributor.author]
Babul, A
Yazar
[dc.contributor.author]
Take, G
Yazar
[dc.contributor.author]
Erdogan, D
Yayın Yılı
[dc.date.issued]
2004
Yayıncı
[dc.publisher]
TAYLOR & FRANCIS LTD
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Nitric oxide (NO) has been reported to function in both cytoprotective and cytotoxic tissue ischemia-reperfusion (I/R). In this study, we evaluated the effects of L-arginine, the substrate for NO, and N-G-nitro L-argine methyl ester (L-NAME), NO synthase (NOS) inhibitor on superoxide dismutase (SOD) enzyme activity, malondialdehyde (MDA), a marker of lipid peroxidation, nitrate levels, and histopathological structure in rat sciatic nerve 2 h after ischemia, followed by 3 h of reperfusion. Reperfusion resulted in a significant increase in lipid peroxidation level and a decrease in nitrate level of the sciatic nerve. The increased level of lipid peroxidation was partly reduced by NOS inhibition. The decrease in sciatic nerve SOD level, observed in group subjected to I/R, was prevented by inhibition of NOS by L-NAME. These results were supported by histological findings that in the L-arginine-treated group, degenerations of both myelin sheath and axon were observed, while in the L-NAME-treated group, no pathological changes were detected. Our results suggested that excessive NO formation accelerates lipid peroxidation, as well as axonal degeneration on the early reperfusion period of the sciatic nerve.
Açıklama
[dc.description]
WOS: 000189260100005
Açıklama
[dc.description]
PubMed: 14754660
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
ischemia
Konu Başlıkları
[dc.subject]
nitric oxide
Konu Başlıkları
[dc.subject]
oxidative stress
Konu Başlıkları
[dc.subject]
reperfusion
Konu Başlıkları
[dc.subject]
sciatic nerve
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0020-7454
İlk Sayfa Sayısı
[dc.identifier.startpage]
349
Son Sayfa Sayısı
[dc.identifier.endpage]
364
Dergi Adı
[dc.relation.journal]
INTERNATIONAL JOURNAL OF NEUROSCIENCE
Dergi Sayısı
[dc.identifier.issue]
3
Dergi Cilt Bilgisi
[dc.identifier.volume]
114
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1080/00207450490270578
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2496
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
148
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
15 Temmuz 2024 01:46
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Tıklayınız
sciatic peroxidation results observed nitrate decrease reperfusion inhibition sheath excessive formation accelerates reduced partly increased axonal degeneration period suggested detected myelin degenerations L-arginine-treated significant findings histological supported L-NAME-treated changes L-NAME prevented subjected pathological increase Nitric
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