An Experimental Study of Radiation Effect on Normal Tissue: Analysis of HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 Expression

Objective: This study investigated whether or not the stress and hypoxia, which are the effects of radiation on normal vascular endothelium, leading to the release of HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 were related and the possibility of them stimulating angiogenesis. Materials and Methods: Twenty-four male Swiss Albino mice were separated into 4 groups. The first group was the control group (Group 1), and the second, third, and fourth groups were euthanized after 24 h (Group 2), 48 h (Group 3), and 7 days (Group 4), respectively. A single-fractioned 10 Gy of ionizing radiation was applied to all mice's pelvic zone with Co-60. Bladders were removed completely from the pelvic region. Immunohistochemistry and light microscopy were used to investigate whether there would be an increase or not in the angiogenesis pathway by using the HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 antibodies. Results: The HIP-1 alpha antibody showed strong staining in Group 3, while the staining intensity was less in other groups. VEGF showed weak staining in Groups 1 and 4, while moderate staining in Group 2 and strong staining in Group 3 was observed. eIF2 showed strong staining in Groups 1 and 4. Groups 2 and 3 were stained weakly. In the present study, staining with TSP-1 was very strong in the samples belonging to Group 1, while other groups showed very weak staining. Conclusion: When normal tissue was exposed to radiation, the positively effective factors (HIF-1, VEGF, eIF2, and TIA-1) on the angiogenesis pathway were increased while the negative factor (TSP-1) was decreased. Radiation may initiate physiological angiogenesis in the normal tissue and accelerate healing in the damaged normal tissue.

Yazar Aktas, Caner
Kurtman, Cengiz
Ozbilgin, M. Kemal
Tek, Ibrahim
Toprak, Selami Kocak
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2054
Tek Biçim Adres 10.4274/Tjh.2012.0142
Konu Başlıkları Angiogenesis
Radiation
Cancer and normal tissue
Vascular endothelium
HIP-1 alpha
VEGF
eIF2
TIA-1
TSP-1
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı TURKISH JOURNAL OF HEMATOLOGY
Dergi Cilt Bilgisi 30
Dergi Sayısı 4
Sayfalar 371 - 378
Yayın Yılı 2013
Eser Adı
[dc.title]
An Experimental Study of Radiation Effect on Normal Tissue: Analysis of HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 Expression
Yazar
[dc.contributor.author]
Aktas, Caner
Yazar
[dc.contributor.author]
Kurtman, Cengiz
Yazar
[dc.contributor.author]
Ozbilgin, M. Kemal
Yazar
[dc.contributor.author]
Tek, Ibrahim
Yazar
[dc.contributor.author]
Toprak, Selami Kocak
Yayın Yılı
[dc.date.issued]
2013
Yayıncı
[dc.publisher]
GALENOS YAYINCILIK
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Objective: This study investigated whether or not the stress and hypoxia, which are the effects of radiation on normal vascular endothelium, leading to the release of HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 were related and the possibility of them stimulating angiogenesis. Materials and Methods: Twenty-four male Swiss Albino mice were separated into 4 groups. The first group was the control group (Group 1), and the second, third, and fourth groups were euthanized after 24 h (Group 2), 48 h (Group 3), and 7 days (Group 4), respectively. A single-fractioned 10 Gy of ionizing radiation was applied to all mice's pelvic zone with Co-60. Bladders were removed completely from the pelvic region. Immunohistochemistry and light microscopy were used to investigate whether there would be an increase or not in the angiogenesis pathway by using the HIF-1 alpha, VEGF, eIF2, TIA-1, and TSP-1 antibodies. Results: The HIP-1 alpha antibody showed strong staining in Group 3, while the staining intensity was less in other groups. VEGF showed weak staining in Groups 1 and 4, while moderate staining in Group 2 and strong staining in Group 3 was observed. eIF2 showed strong staining in Groups 1 and 4. Groups 2 and 3 were stained weakly. In the present study, staining with TSP-1 was very strong in the samples belonging to Group 1, while other groups showed very weak staining. Conclusion: When normal tissue was exposed to radiation, the positively effective factors (HIF-1, VEGF, eIF2, and TIA-1) on the angiogenesis pathway were increased while the negative factor (TSP-1) was decreased. Radiation may initiate physiological angiogenesis in the normal tissue and accelerate healing in the damaged normal tissue.
Açıklama
[dc.description]
WOS: 000331341700005
Açıklama
[dc.description]
PubMed: 24385827
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Angiogenesis
Konu Başlıkları
[dc.subject]
Radiation
Konu Başlıkları
[dc.subject]
Cancer and normal tissue
Konu Başlıkları
[dc.subject]
Vascular endothelium
Konu Başlıkları
[dc.subject]
HIP-1 alpha
Konu Başlıkları
[dc.subject]
VEGF
Konu Başlıkları
[dc.subject]
eIF2
Konu Başlıkları
[dc.subject]
TIA-1
Konu Başlıkları
[dc.subject]
TSP-1
Haklar
[dc.rights]
info:eu-repo/semantics/openAccess
ISSN
[dc.identifier.issn]
1300-7777
ISSN
[dc.identifier.issn]
1308-5263
İlk Sayfa Sayısı
[dc.identifier.startpage]
371
Son Sayfa Sayısı
[dc.identifier.endpage]
378
Dergi Adı
[dc.relation.journal]
TURKISH JOURNAL OF HEMATOLOGY
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
30
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.4274/Tjh.2012.0142
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2054
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
56
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
18 Mayıs 2024 15:40
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Tıklayınız
staining groups normal angiogenesis (Group strong showed tissue radiation Groups pelvic whether pathway antibody increase intensity antibodies Results Objective samples moderate TIA-1) damaged healing accelerate physiological initiate Radiation decreased (TSP-1) factor negative increased (HIF-1 observed
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