Increased Serum Levels of IL-28 and IL-29 and the Protective Effect of IL28B rs8099917 Polymorphism in Patients with Hashimoto's Thyroiditis

Hashimoto's thyroiditis (HT) is thought to result from decreased T helper type 2 (Th2) responses, leading to the progressive destruction of thyrocytes. IFN-1, -2, and -3 (also known as IL-29, IL-28A, and IL-28B, respectively) are recently described members of the IFN- family and have been shown to decrease the production of Th2 cytokines in vitro. However, the role and mechanism of IFN-1 in HT remain unknown. The purpose of this study was to examine whether IL29 and IL28B gene polymorphisms are susceptibility genes for the development of HT. Also, we investigated the effects of IL-29 and IL-28 serum levels in the pathogenesis of HT. Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, single-nucleotide polymorphisms (SNPs) of IL28B rs8099917 (IL28 G/T) and IL29 rs30461 (IL29 T/C) were studied in 99 patients with HT and 100 healthy controls. Considering the allelic distribution of the IL28 G/T polymorphism, a higher frequency of the G allele was observed in the control group versus the HT group. Thus, it was suggested that the G allele may be protective against HT pathogenesis (OR = 0.388, 95% CI = 0.217-0.693; p = 0.001). Our findings also demonstrated that there was a statistically significant difference in serum IL-28 and IL-29 levels between case and control groups (p < 0.001). Increased serum levels of IL-28 and IL-29 were found in patients with HT. However, we did not find a relationship between the IL29 gene polymorphism and HT. In conclusion, the IL28B gene polymorphism and serum IL-28 and IL-29 levels seem to play a role in the pathogenesis of HT.

Yazar Arpaci, Dilek
Celik, Sevim Karakas
Can, Murat
Genc, Gunes Cakmak
Kuzu, Fatih
Unal, Mustafa
Bayraktaroglu, Taner
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2771
Tek Biçim Adres 10.1080/08820139.2016.1208215
Konu Başlıkları Hashimoto's thyroiditis
IL28
IL29
polymorphism
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı IMMUNOLOGICAL INVESTIGATIONS
Dergi Cilt Bilgisi 45
Dergi Sayısı 7
Sayfalar 668 - 678
Yayın Yılı 2016
Eser Adı
[dc.title]
Increased Serum Levels of IL-28 and IL-29 and the Protective Effect of IL28B rs8099917 Polymorphism in Patients with Hashimoto's Thyroiditis
Yazar
[dc.contributor.author]
Arpaci, Dilek
Yazar
[dc.contributor.author]
Celik, Sevim Karakas
Yazar
[dc.contributor.author]
Can, Murat
Yazar
[dc.contributor.author]
Genc, Gunes Cakmak
Yazar
[dc.contributor.author]
Kuzu, Fatih
Yazar
[dc.contributor.author]
Unal, Mustafa
Yazar
[dc.contributor.author]
Bayraktaroglu, Taner
Yayın Yılı
[dc.date.issued]
2016
Yayıncı
[dc.publisher]
TAYLOR & FRANCIS INC
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Hashimoto's thyroiditis (HT) is thought to result from decreased T helper type 2 (Th2) responses, leading to the progressive destruction of thyrocytes. IFN-1, -2, and -3 (also known as IL-29, IL-28A, and IL-28B, respectively) are recently described members of the IFN- family and have been shown to decrease the production of Th2 cytokines in vitro. However, the role and mechanism of IFN-1 in HT remain unknown. The purpose of this study was to examine whether IL29 and IL28B gene polymorphisms are susceptibility genes for the development of HT. Also, we investigated the effects of IL-29 and IL-28 serum levels in the pathogenesis of HT. Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, single-nucleotide polymorphisms (SNPs) of IL28B rs8099917 (IL28 G/T) and IL29 rs30461 (IL29 T/C) were studied in 99 patients with HT and 100 healthy controls. Considering the allelic distribution of the IL28 G/T polymorphism, a higher frequency of the G allele was observed in the control group versus the HT group. Thus, it was suggested that the G allele may be protective against HT pathogenesis (OR = 0.388, 95% CI = 0.217-0.693; p = 0.001). Our findings also demonstrated that there was a statistically significant difference in serum IL-28 and IL-29 levels between case and control groups (p < 0.001). Increased serum levels of IL-28 and IL-29 were found in patients with HT. However, we did not find a relationship between the IL29 gene polymorphism and HT. In conclusion, the IL28B gene polymorphism and serum IL-28 and IL-29 levels seem to play a role in the pathogenesis of HT.
Açıklama
[dc.description]
WOS: 000384678700007
Açıklama
[dc.description]
PubMed: 27617784
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Hashimoto's thyroiditis
Konu Başlıkları
[dc.subject]
IL28
Konu Başlıkları
[dc.subject]
IL29
Konu Başlıkları
[dc.subject]
polymorphism
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0882-0139
ISSN
[dc.identifier.issn]
1532-4311
İlk Sayfa Sayısı
[dc.identifier.startpage]
668
Son Sayfa Sayısı
[dc.identifier.endpage]
678
Dergi Adı
[dc.relation.journal]
IMMUNOLOGICAL INVESTIGATIONS
Dergi Sayısı
[dc.identifier.issue]
7
Dergi Cilt Bilgisi
[dc.identifier.volume]
45
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1080/08820139.2016.1208215
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2771
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
23
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
08 Şubat 2024 10:37
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Tıklayınız
levels polymorphism pathogenesis allele control polymorphisms However between patients higher observed versus studied healthy controls Considering allelic distribution frequency Hashimoto groups Increased significant relationship conclusion difference statistically suggested rs30461 protective against findings demonstrated polymerase destruction
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