Effect of adrenomedullin on hepatic damage in hepatic ischaemia/reperfusion injury in rats

Aims: Adrenomedullin (AM) is a multifunctional peptide with a putative beneficial role after an ischaemic insult. The aim of this study was to evaluate the effect of AM on partial hepatic ischaemia reperfusion (I/R) injury. Methods: Rats were subjected to 1 h of 70% hepatic ischaemia, followed by reperfusion or sham. At the end of ischaemia, vehicle (phosphate-buffered saline solution), N-nitro-L-arginine methyl ester (L-NAME) and AM with or without L-NAME were infused via the portal vein. Analysis was performed at pre-ischaemia, ischaemia onset and 1, 2 and 4 h after reperfusion. Hepatic tissue blood flow (HTBF) was evaluated by laser Doppler. Results: Plasma AM levels in the I/R groups were significantly lower than the levels in the sham group. AM treatment significantly reduced levels of aspartate transaminase and tissue arginase (P < 0.05). Significant decreases of tumour necrosis factor-alpha, interleukin-1 beta and endothelin-1 levels were also found in the serum. Endothelin-1, malondialdehyde and necrosis were observed more frequently in liver tissue in the AM group than the control (P < 0.05). Tissue nitric oxide, energy charge and HTBF were significantly increased in AM treatment experiments (P < 0.05). Conclusion: The improved HTBF, energy charge and nitric oxide and the reduction of hepatic necrosis, oxidative stress, liver enzymes, endotelin-1 and pro-inflammatory cytokines demonstrate that treatment with AM attenuates liver I/R injury.

Yazar Kerem, Mustafa
Bedirli, Abdulkadir
Pasaoglu, Hatice
Ofluoglu, Ebru
Yilmazer, Demet
Salman, Bulent
Yilmaz, Tonguc Utku
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2437
Tek Biçim Adres 10.1111/j.1478-3231.2008.01741.x
Konu Başlıkları adrenomedullin
endothelin
ischaemia
liver
nitric oxide
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı LIVER INTERNATIONAL
Dergi Cilt Bilgisi 28
Dergi Sayısı 7
Sayfalar 972 - 981
Yayın Yılı 2008
Eser Adı
[dc.title]
Effect of adrenomedullin on hepatic damage in hepatic ischaemia/reperfusion injury in rats
Yazar
[dc.contributor.author]
Kerem, Mustafa
Yazar
[dc.contributor.author]
Bedirli, Abdulkadir
Yazar
[dc.contributor.author]
Pasaoglu, Hatice
Yazar
[dc.contributor.author]
Ofluoglu, Ebru
Yazar
[dc.contributor.author]
Yilmazer, Demet
Yazar
[dc.contributor.author]
Salman, Bulent
Yazar
[dc.contributor.author]
Yilmaz, Tonguc Utku
Yayın Yılı
[dc.date.issued]
2008
Yayıncı
[dc.publisher]
WILEY-BLACKWELL
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Aims: Adrenomedullin (AM) is a multifunctional peptide with a putative beneficial role after an ischaemic insult. The aim of this study was to evaluate the effect of AM on partial hepatic ischaemia reperfusion (I/R) injury. Methods: Rats were subjected to 1 h of 70% hepatic ischaemia, followed by reperfusion or sham. At the end of ischaemia, vehicle (phosphate-buffered saline solution), N-nitro-L-arginine methyl ester (L-NAME) and AM with or without L-NAME were infused via the portal vein. Analysis was performed at pre-ischaemia, ischaemia onset and 1, 2 and 4 h after reperfusion. Hepatic tissue blood flow (HTBF) was evaluated by laser Doppler. Results: Plasma AM levels in the I/R groups were significantly lower than the levels in the sham group. AM treatment significantly reduced levels of aspartate transaminase and tissue arginase (P < 0.05). Significant decreases of tumour necrosis factor-alpha, interleukin-1 beta and endothelin-1 levels were also found in the serum. Endothelin-1, malondialdehyde and necrosis were observed more frequently in liver tissue in the AM group than the control (P < 0.05). Tissue nitric oxide, energy charge and HTBF were significantly increased in AM treatment experiments (P < 0.05). Conclusion: The improved HTBF, energy charge and nitric oxide and the reduction of hepatic necrosis, oxidative stress, liver enzymes, endotelin-1 and pro-inflammatory cytokines demonstrate that treatment with AM attenuates liver I/R injury.
Açıklama
[dc.description]
WOS: 000257706600009
Açıklama
[dc.description]
PubMed: 18435717
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
adrenomedullin
Konu Başlıkları
[dc.subject]
endothelin
Konu Başlıkları
[dc.subject]
ischaemia
Konu Başlıkları
[dc.subject]
liver
Konu Başlıkları
[dc.subject]
nitric oxide
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1478-3223
İlk Sayfa Sayısı
[dc.identifier.startpage]
972
Son Sayfa Sayısı
[dc.identifier.endpage]
981
Dergi Adı
[dc.relation.journal]
LIVER INTERNATIONAL
Dergi Sayısı
[dc.identifier.issue]
7
Dergi Cilt Bilgisi
[dc.identifier.volume]
28
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1111/j.1478-3231.2008.01741.x
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2437
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
8
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
06 Şubat 2024 14:32
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Tıklayınız
levels ischaemia hepatic significantly treatment reperfusion necrosis tissue injury nitric energy charge transaminase attenuates oxidative groups stress enzymes arginase endotelin-1 aspartate reduced pro-inflammatory decreases cytokines demonstrate Significant improved tumour observed Tissue control increased frequently experiments
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