FIRST ISOLATION AND DETECTION OF MULTIPLE CLONES OF VANCOMYCIN-RESISTANT ENTEROCOCCI IN THE PEDIATRIC UNIT OF VAN YUZUNCU YIL UNIVERSITY, TURKEY

Upon isolation of the first vancomycin resistant enterococcus (VRE) from the urine sample of a nine months old patient in pediatric unit of Van Yuzuncu Yil University Hospital (located in eastern part of Turkey), we aimed to search for the presence of VRE isolates in the unit, to determine the resistance genotypes and to evaluate the clonal relationships among isolates. A total of 28 rectal swabs and 28 skin swabs from the patients, 12 skin swabs from the staff giving care to the patients, 15 skin swabs from the mothers of the patients and 96 environmental samples from the pediatric unit were screened. Antibiotic susceptibilities were tested and the resistance genotypes were determined. Molecular typing of the isolates was performed using pulsed-field gel electrophoresis (PFGE). Apart from the first case, 13 more VRE isolates, one being a clinical isolate from the urine of a patient and 12 isolates from the screening samples (8 rectal swabs, one skin swab and three swabs from patients' beds) were obtained. All of the isolates were identified as Enterococcus faecium with similar antibiotic susceptibility patterns. VanA gene was present in all of the isolates. PFGE demonstrated two major clones and five clones closely related with the major ones. This was the first VRE isolation and colonization reported in our region. The isolates belonged to more than one clone. Currently, VRE did not seem to be a significant pathogen in Turkey, however, there may be an underestimation of the problem and continuous surveillance studies should be undertaken in every region.

Yazar Guducuoglu, Huseyin
Aktas, Elif
Comert, Fusun Begendik
Aygul, Kumru
Ozlu, Nagihan
Baykal, Sanem
Berktas, Mustafa
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/2655
Konu Başlıkları Vancomycin resistant enterococcus
nosocomial infection
clonal relation
pulsed-field gel electrophoresis
Turkey
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı MIKROBIYOLOJI BULTENI
Dergi Cilt Bilgisi 43
Dergi Sayısı 4
Sayfalar 535 - 543
Yayın Yılı 2009
Eser Adı
[dc.title]
FIRST ISOLATION AND DETECTION OF MULTIPLE CLONES OF VANCOMYCIN-RESISTANT ENTEROCOCCI IN THE PEDIATRIC UNIT OF VAN YUZUNCU YIL UNIVERSITY, TURKEY
Yazar
[dc.contributor.author]
Guducuoglu, Huseyin
Yazar
[dc.contributor.author]
Aktas, Elif
Yazar
[dc.contributor.author]
Comert, Fusun Begendik
Yazar
[dc.contributor.author]
Aygul, Kumru
Yazar
[dc.contributor.author]
Ozlu, Nagihan
Yazar
[dc.contributor.author]
Baykal, Sanem
Yazar
[dc.contributor.author]
Berktas, Mustafa
Yayın Yılı
[dc.date.issued]
2009
Yayıncı
[dc.publisher]
ANKARA MICROBIOLOGY SOC
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Upon isolation of the first vancomycin resistant enterococcus (VRE) from the urine sample of a nine months old patient in pediatric unit of Van Yuzuncu Yil University Hospital (located in eastern part of Turkey), we aimed to search for the presence of VRE isolates in the unit, to determine the resistance genotypes and to evaluate the clonal relationships among isolates. A total of 28 rectal swabs and 28 skin swabs from the patients, 12 skin swabs from the staff giving care to the patients, 15 skin swabs from the mothers of the patients and 96 environmental samples from the pediatric unit were screened. Antibiotic susceptibilities were tested and the resistance genotypes were determined. Molecular typing of the isolates was performed using pulsed-field gel electrophoresis (PFGE). Apart from the first case, 13 more VRE isolates, one being a clinical isolate from the urine of a patient and 12 isolates from the screening samples (8 rectal swabs, one skin swab and three swabs from patients' beds) were obtained. All of the isolates were identified as Enterococcus faecium with similar antibiotic susceptibility patterns. VanA gene was present in all of the isolates. PFGE demonstrated two major clones and five clones closely related with the major ones. This was the first VRE isolation and colonization reported in our region. The isolates belonged to more than one clone. Currently, VRE did not seem to be a significant pathogen in Turkey, however, there may be an underestimation of the problem and continuous surveillance studies should be undertaken in every region.
Açıklama
[dc.description]
WOS: 000271840400003
Açıklama
[dc.description]
PubMed: 20084906
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
tur
Konu Başlıkları
[dc.subject]
Vancomycin resistant enterococcus
Konu Başlıkları
[dc.subject]
nosocomial infection
Konu Başlıkları
[dc.subject]
clonal relation
Konu Başlıkları
[dc.subject]
pulsed-field gel electrophoresis
Konu Başlıkları
[dc.subject]
Turkey
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0374-9096
İlk Sayfa Sayısı
[dc.identifier.startpage]
535
Son Sayfa Sayısı
[dc.identifier.endpage]
543
Dergi Adı
[dc.relation.journal]
MIKROBIYOLOJI BULTENI
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
43
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/2655
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
35
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
10 Şubat 2024 19:12
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Tıklayınız
isolates patients samples genotypes resistance rectal clones region patient isolation pediatric obtained identified Enterococcus closely patterns demonstrated similar antibiotic present susceptibility faecium related pathogen undertaken should studies surveillance continuous problem underestimation however Turkey significant Currently
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