Effects of humanin on experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid in rats

Background/Aim: The excessive apoptosis of intestinal epithelial cells (IECs) partly accounts for the development of colonic inflammation and eventually results in ulcerative colitis (UC). Humanin, an endogenous anti-apoptotic peptide, has previously been shown to protect against Alzheimer's disease and a variety of cellular insults. The present study aimed to investigate the effects of glysin variant of humanin (HNG) on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. Materials and Methods: Rats were divided into four groups as follows: Group 1 (n = 8): control; isotonic saline solution 0.1 ml/rat rectally, Group 2 (n = 8): TNBS colitis; 0.1 ml of a 2.5% (w/v) TNBS solution in 50% ethanol rectally, Group 3 (n = 8): 10 µM HNG, and Group 4 (n = 8): 20 µM HNG intraperitoneal (ip) on day 2 and 6 after rectal TNBS administration. Rats were sacrificed 7 days after the induction of colitis. Blood and tissue samples were harvested for biochemical and histopathological analysis. Results: HNG treatment significantly ameliorated weight loss and macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic mRNA expression of tumor necrosis factor-alpha (TNF-?), interleukin-1beta (IL-1ß), and caspase-3 activities in group II in comparison to the group I. HNG treatment was associated with an inhibition of mRNA expression of TNF-? and IL-1ß, and a decrease in caspase-3 activities in colon tissues in group III and IV when compared to group II. Conclusion: The results of this study indicate that HNG treatment may exert beneficial effects in UC by decreasing inflammatory reactions and apoptosis. © 2017 Saudi Journal of Gastroenterology (Official journal of The Saudi Gastroenterology Association) | Published by Wolters Kluwer - Medknow.

Dergi Adı Saudi Journal of Gastroenterology
Dergi Cilt Bilgisi 23
Dergi Sayısı 2
Sayfalar 105 - 111
Yayın Yılı 2017
Eser Adı
[dc.title]
Effects of humanin on experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid in rats
Yazar
[dc.contributor.author]
Gultekin F.A.
Yazar
[dc.contributor.author]
Emre A.U.
Yazar
[dc.contributor.author]
Celik S.K.
Yazar
[dc.contributor.author]
Barut F.
Yazar
[dc.contributor.author]
Tali U.
Yazar
[dc.contributor.author]
Sumer D.
Yazar
[dc.contributor.author]
Turkcu U.O.
Yayın Yılı
[dc.date.issued]
2017
Yayıncı
[dc.publisher]
Medknow Publications
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background/Aim: The excessive apoptosis of intestinal epithelial cells (IECs) partly accounts for the development of colonic inflammation and eventually results in ulcerative colitis (UC). Humanin, an endogenous anti-apoptotic peptide, has previously been shown to protect against Alzheimer's disease and a variety of cellular insults. The present study aimed to investigate the effects of glysin variant of humanin (HNG) on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. Materials and Methods: Rats were divided into four groups as follows: Group 1 (n = 8): control; isotonic saline solution 0.1 ml/rat rectally, Group 2 (n = 8): TNBS colitis; 0.1 ml of a 2.5% (w/v) TNBS solution in 50% ethanol rectally, Group 3 (n = 8): 10 µM HNG, and Group 4 (n = 8): 20 µM HNG intraperitoneal (ip) on day 2 and 6 after rectal TNBS administration. Rats were sacrificed 7 days after the induction of colitis. Blood and tissue samples were harvested for biochemical and histopathological analysis. Results: HNG treatment significantly ameliorated weight loss and macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic mRNA expression of tumor necrosis factor-alpha (TNF-?), interleukin-1beta (IL-1ß), and caspase-3 activities in group II in comparison to the group I. HNG treatment was associated with an inhibition of mRNA expression of TNF-? and IL-1ß, and a decrease in caspase-3 activities in colon tissues in group III and IV when compared to group II. Conclusion: The results of this study indicate that HNG treatment may exert beneficial effects in UC by decreasing inflammatory reactions and apoptosis. © 2017 Saudi Journal of Gastroenterology (Official journal of The Saudi Gastroenterology Association) | Published by Wolters Kluwer - Medknow.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Apoptosis
Konu Başlıkları
[dc.subject]
humanin
Konu Başlıkları
[dc.subject]
ulcerative colitis
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1319-3767
İlk Sayfa Sayısı
[dc.identifier.startpage]
105
Son Sayfa Sayısı
[dc.identifier.endpage]
111
Dergi Adı
[dc.relation.journal]
Saudi Journal of Gastroenterology
Dergi Sayısı
[dc.identifier.issue]
2
Dergi Cilt Bilgisi
[dc.identifier.volume]
23
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.4103/sjg.SJG_318_16
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5483
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
38
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 08:22
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colitis treatment effects solution expression caspase-3 rectally activities Gastroenterology significantly apoptosis colonic results analysis scores (TNF-?) interleukin-1beta (IL-1ß) biochemical harvested histopathological ameliorated Results TNBS-induced factor-alpha necrosis weight macroscopic increased microscopic Background/Aim comparison (Official decreasing inflammatory
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