Oxidized low-density lipoproteins accumulate in rat lung after experimental lung edema induced by alpha- naphthylthiourea (ANTU)

Oxidation of the low-density lipoprotein (LDL) results in the production of modified LDLs. Oxidation of LDL cholesterol plays a role on the pathogenesis of endothelial dysfunction. This study was designed to investigate the possible participation of the oxidative modification of low density lipoprotein in the lung edema induced by alpha-naphthylthiourea (ANTU), which is a well-known noxious chemical agent on the lung endothelium. When ANTU injected intraperitoneally into rats (15 mg kg-1), it produced lung edema as indicated by an increase in lung weight/body weight (LW/BW) ratio and pleural effusion (PE) reaching a maximum within 4 h. A significant lung edema was observed 4 h after intraperitoneally injection of alpha-naphthylthiourea when compared with olive oil-injected control rats. On microscopic examination of alpha-naphthylthiourea-treated rats were shown to have severe lung injury, while no change was observed in olive oil-treated control rats. While there were no staining in control lungs, positive oxidized low-density lipoproteins immune-fluorescent staining were observed in lung edema group. Our study showed that oxidized low-density lipoprotein (oxLDL) accumulated in ANTU-induced lung damage. This is the first study in which accumulation of oxLDL molecules in the intact lung tissue were shown by fluorescent immune-staining method in experimental lung edema. The potential role of oxLDL in this pathology are still under investigation. © 2004 Elsevier Ltd. All rights reserved.

Dergi Adı Pharmacological Research
Dergi Cilt Bilgisi 50
Dergi Sayısı 6
Sayfalar 585 - 591
Yayın Yılı 2004
Eser Adı
[dc.title]
Oxidized low-density lipoproteins accumulate in rat lung after experimental lung edema induced by alpha- naphthylthiourea (ANTU)
Yazar
[dc.contributor.author]
Sipahi E.Y.
Yazar
[dc.contributor.author]
Ozel Tekin I.
Yazar
[dc.contributor.author]
Comert M.
Yazar
[dc.contributor.author]
Barut F.
Yazar
[dc.contributor.author]
Ustun H.
Yazar
[dc.contributor.author]
Sipahi T.H.
Yayın Yılı
[dc.date.issued]
2004
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Oxidation of the low-density lipoprotein (LDL) results in the production of modified LDLs. Oxidation of LDL cholesterol plays a role on the pathogenesis of endothelial dysfunction. This study was designed to investigate the possible participation of the oxidative modification of low density lipoprotein in the lung edema induced by alpha-naphthylthiourea (ANTU), which is a well-known noxious chemical agent on the lung endothelium. When ANTU injected intraperitoneally into rats (15 mg kg-1), it produced lung edema as indicated by an increase in lung weight/body weight (LW/BW) ratio and pleural effusion (PE) reaching a maximum within 4 h. A significant lung edema was observed 4 h after intraperitoneally injection of alpha-naphthylthiourea when compared with olive oil-injected control rats. On microscopic examination of alpha-naphthylthiourea-treated rats were shown to have severe lung injury, while no change was observed in olive oil-treated control rats. While there were no staining in control lungs, positive oxidized low-density lipoproteins immune-fluorescent staining were observed in lung edema group. Our study showed that oxidized low-density lipoprotein (oxLDL) accumulated in ANTU-induced lung damage. This is the first study in which accumulation of oxLDL molecules in the intact lung tissue were shown by fluorescent immune-staining method in experimental lung edema. The potential role of oxLDL in this pathology are still under investigation. © 2004 Elsevier Ltd. All rights reserved.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Alpha-naphthylthiourea
Konu Başlıkları
[dc.subject]
Lung edema
Konu Başlıkları
[dc.subject]
Oxidized low-density lipoprotein
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1043-6618
Sponsor YAYINCI
[dc.description.sponsorship]
The authors would like to thank to Dr. E. Schilliger, Schering AG, Berlin, Germany, for his gift of alpha-naphthylthiourea (Interchem). We thank Hasan Tahsin Yilmaz and Ramazan Temel for their assistance in research laboratory. This work was supported by intramural funding from Zonguldak Karaelmas University’s Office of Research and Sponsored Programs.
İlk Sayfa Sayısı
[dc.identifier.startpage]
585
Son Sayfa Sayısı
[dc.identifier.endpage]
591
Dergi Adı
[dc.relation.journal]
Pharmacological Research
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
50
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.phrs.2004.04.003
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/6908
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
31
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
08 Şubat 2024 05:57
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observed low-density lipoprotein control oxidized staining alpha-naphthylthiourea intraperitoneally Oxidation examination immune-fluorescent lipoproteins microscopic positive injury alpha-naphthylthiourea-treated oil-treated change severe molecules showed reserved rights Elsevier investigation pathology potential experimental method immune-staining fluorescent tissue intact accumulation damage
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