Experimental bile-duct ligation resulted in accumulation of oxidized low-density lipoproteins in BALB/c mice liver

Background and Aim: Oxidized low-density lipoproteins (LDL), which are produced during oxidative stress by the process of lipid peroxidation, have also been proposed to have complex roles in many other immuno-inflammatory mechanisms. It has been shown that bile-duct ligation results in oxidative stress in the liver of animals. The aim of this study was to investigate if oxidized LDL are produced in the liver tissues of bile-duct-ligated mice. Methods: Obstructive jaundice was induced in BALB/c mice by the ligation and division of the common bile duct. Liver concentrations of glutathione and malondialdehyde were measured in the sham-operated (n = 10) and bile-duct-ligated (n = 10) mice on the 10th day of obstructive jaundice. The presence of oxidized LDL in the liver tissue sections was evaluated using a special, novel immunofluorescent staining method. The final step was to explore the existence of oxidized LDL under fluorescent microscopy. Results: Compared with sham-operated mice, jaundiced mice showed significantly higher levels of malondialdehyde and lower concentrations of reduced glutathione in the liver. While there was no staining in the sham-operated group, bile-duct ligation resulted in positive oxidized LDL staining in the liver tissues of mice. The present study testifies that bile-duct ligation results in oxidative stress and enhanced lipid peroxidation in the hepatic tissues of BALB/c mice and moreover, that oxidized LDL accumulate in the liver of mice with experimental obstructive jaundice. Conclusion: Oxidized LDL may be an important and direct indicator of ongoing oxidative stress and enhanced lipid peroxidation in obstructive jaundice. The potential roles of this finding were also discussed, briefly. © 2004 Blackwell Publishing Asia Pty Ltd.

Dergi Adı Journal of Gastroenterology and Hepatology (Australia)
Dergi Cilt Bilgisi 19
Dergi Sayısı 9
Sayfalar 1052 - 1057
Yayın Yılı 2004
Eser Adı
[dc.title]
Experimental bile-duct ligation resulted in accumulation of oxidized low-density lipoproteins in BALB/c mice liver
Yazar
[dc.contributor.author]
Cömert M.
Yazar
[dc.contributor.author]
Tekin I.Ö.
Yazar
[dc.contributor.author]
Açikgöz Ş.
Yazar
[dc.contributor.author]
Üstundag Y.
Yazar
[dc.contributor.author]
Uçan B.H.
Yazar
[dc.contributor.author]
Acun Z.
Yazar
[dc.contributor.author]
Barut F.
Yayın Yılı
[dc.date.issued]
2004
Yayıncı
[dc.publisher]
Blackwell Publishing
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background and Aim: Oxidized low-density lipoproteins (LDL), which are produced during oxidative stress by the process of lipid peroxidation, have also been proposed to have complex roles in many other immuno-inflammatory mechanisms. It has been shown that bile-duct ligation results in oxidative stress in the liver of animals. The aim of this study was to investigate if oxidized LDL are produced in the liver tissues of bile-duct-ligated mice. Methods: Obstructive jaundice was induced in BALB/c mice by the ligation and division of the common bile duct. Liver concentrations of glutathione and malondialdehyde were measured in the sham-operated (n = 10) and bile-duct-ligated (n = 10) mice on the 10th day of obstructive jaundice. The presence of oxidized LDL in the liver tissue sections was evaluated using a special, novel immunofluorescent staining method. The final step was to explore the existence of oxidized LDL under fluorescent microscopy. Results: Compared with sham-operated mice, jaundiced mice showed significantly higher levels of malondialdehyde and lower concentrations of reduced glutathione in the liver. While there was no staining in the sham-operated group, bile-duct ligation resulted in positive oxidized LDL staining in the liver tissues of mice. The present study testifies that bile-duct ligation results in oxidative stress and enhanced lipid peroxidation in the hepatic tissues of BALB/c mice and moreover, that oxidized LDL accumulate in the liver of mice with experimental obstructive jaundice. Conclusion: Oxidized LDL may be an important and direct indicator of ongoing oxidative stress and enhanced lipid peroxidation in obstructive jaundice. The potential roles of this finding were also discussed, briefly. © 2004 Blackwell Publishing Asia Pty Ltd.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Liver
Konu Başlıkları
[dc.subject]
Obstructive jaundice
Konu Başlıkları
[dc.subject]
Oxidized low-density lipoprotein
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0815-9319
İlk Sayfa Sayısı
[dc.identifier.startpage]
1052
Son Sayfa Sayısı
[dc.identifier.endpage]
1057
Dergi Adı
[dc.relation.journal]
Journal of Gastroenterology and Hepatology (Australia)
Dergi Sayısı
[dc.identifier.issue]
9
Dergi Cilt Bilgisi
[dc.identifier.volume]
19
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1111/j.1440-1746.2004.03400.x
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5822
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
37
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
13 Şubat 2024 17:50
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oxidized stress ligation oxidative jaundice tissues sham-operated bile-duct peroxidation staining obstructive results enhanced BALB/c malondialdehyde bile-duct-ligated glutathione concentrations Oxidized produced Blackwell Publishing reduced levels higher significantly showed jaundiced Compared Results microscopy potential direct indicator ongoing
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