Expression of heat shock protein 70 and endothelial nitric oxide synthase in placental tissue of preeclamptic and intrauterine growth-restricted pregnancies

Ischemia, hypoxia, and elevated vascular resistance disturb placental functions by increasing oxidative stress. Heat shock protein 70 (HSP70) is an oxidative stress marker. Endothelial nitric oxide synthase (eNOS) is a nitric oxide enzyme with a key role in pathologic and physiologic angiogenesis and vasculogenesis. This study was performed to investigate the role of oxidative stress in the pathogenesis of preeclampsia and intrauterine growth-restricted (IUGR) pregnancies by comparing the levels of HSP70 and eNOS in placentas from women with these diseases and those with healthy pregnancies.HSP70 and eNOS were examined using the streptavidin-biotin-peroxidase complex technique on formalin-fixed, paraffin-embedded sections from 135 placental villous tissues obtained from normal pregnancies (n=45) and pregnancies complicated with preeclampsia (n=45) and IUGR (n=45). The intensity of labeling in placental tissues with antibodies to HSP70 and eNOS was scored between 0 and 3, using a semiquantitative scale.HSP70 and eNOS levels were increased in the syncytiotrophoblasts, cytotrophoblasts, and extravillous trophoblast cells of preeclamptic and IUGR placentas (P<0.001), compared with normal pregnancies. However, their levels were increased only in the villous endothelial cells of IUGR placentas (P<0.001).Oxidative stress is thought to play an important role in the pathogenesis of preeclampsia and IUGR pregnancies. © 2010 Elsevier GmbH.

Dergi Adı Pathology Research and Practice
Dergi Cilt Bilgisi 206
Dergi Sayısı 9
Sayfalar 651 - 656
Yayın Yılı 2010
Eser Adı
[dc.title]
Expression of heat shock protein 70 and endothelial nitric oxide synthase in placental tissue of preeclamptic and intrauterine growth-restricted pregnancies
Yazar
[dc.contributor.author]
Barut F.
Yazar
[dc.contributor.author]
Barut A.
Yazar
[dc.contributor.author]
Dogan Gun B.
Yazar
[dc.contributor.author]
Kandemir N.O.
Yazar
[dc.contributor.author]
Aktunc E.
Yazar
[dc.contributor.author]
Harma M.
Yazar
[dc.contributor.author]
Harma M.I.
Yayın Yılı
[dc.date.issued]
2010
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Ischemia, hypoxia, and elevated vascular resistance disturb placental functions by increasing oxidative stress. Heat shock protein 70 (HSP70) is an oxidative stress marker. Endothelial nitric oxide synthase (eNOS) is a nitric oxide enzyme with a key role in pathologic and physiologic angiogenesis and vasculogenesis. This study was performed to investigate the role of oxidative stress in the pathogenesis of preeclampsia and intrauterine growth-restricted (IUGR) pregnancies by comparing the levels of HSP70 and eNOS in placentas from women with these diseases and those with healthy pregnancies.HSP70 and eNOS were examined using the streptavidin-biotin-peroxidase complex technique on formalin-fixed, paraffin-embedded sections from 135 placental villous tissues obtained from normal pregnancies (n=45) and pregnancies complicated with preeclampsia (n=45) and IUGR (n=45). The intensity of labeling in placental tissues with antibodies to HSP70 and eNOS was scored between 0 and 3, using a semiquantitative scale.HSP70 and eNOS levels were increased in the syncytiotrophoblasts, cytotrophoblasts, and extravillous trophoblast cells of preeclamptic and IUGR placentas (P<0.001), compared with normal pregnancies. However, their levels were increased only in the villous endothelial cells of IUGR placentas (P<0.001).Oxidative stress is thought to play an important role in the pathogenesis of preeclampsia and IUGR pregnancies. © 2010 Elsevier GmbH.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Endothelial nitric oxide synthase
Konu Başlıkları
[dc.subject]
Heat shock protein 70
Konu Başlıkları
[dc.subject]
Intrauterine growth restriction
Konu Başlıkları
[dc.subject]
Placenta
Konu Başlıkları
[dc.subject]
Preeclampsia
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0344-0338
İlk Sayfa Sayısı
[dc.identifier.startpage]
651
Son Sayfa Sayısı
[dc.identifier.endpage]
656
Dergi Adı
[dc.relation.journal]
Pathology Research and Practice
Dergi Sayısı
[dc.identifier.issue]
9
Dergi Cilt Bilgisi
[dc.identifier.volume]
206
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.prp.2010.04.001
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5842
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
36
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
10 Şubat 2024 00:42
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Tıklayınız
pregnancies stress placentas levels preeclampsia placental oxidative (n=45) villous tissues increased normal nitric pathogenesis complicated labeling antibodies scored between obtained intensity cytotrophoblasts Elsevier important thought Oxidative endothelial However compared preeclamptic trophoblast extravillous sections syncytiotrophoblasts semiquantitative
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