Effect of dexmedetomidine on testicular torsion/detorsion damage in rats

Background and Objective: We assessed the antioxidant activity of dexmedetomidine (DEX) during an ischemic period in a rat model of testicular torsion/detorsion (T/DT) by using biochemical and histopathological methods. Methods: Wistar Albino male rats weighing 250-300 g were divided into three groups: sham (group S, n = 7); torsion/detorsion (group T/DT, n = 7), and DEX treatment (group DEX, n = 7). In the T/DT group, right testes were rotated 720° for 1 h. Group S served for normal basal values. Rats in group T/DT were operated to make T/DT, this group served as a control group. Group DEX received intraperitoneal DEX 10 µg · kg-1 after the 30-min torsion period. For measurement of total antioxidant enzyme activities and malondialdehyde (MDA) levels, testes of 7 animals in each group were excised after 4 h of reperfusion. Germ cell apoptosis was evaluated using the apoptosis protease-activating factor 1 (APAF-1) antibody in all groups and also on the expressions of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) were assessed within the bilateral testes. Results: Mean MDA levels in group T/DT were significantly higher than in groups S and DEX (p < 0.05). There were also significant decreases in mean total antioxidant activities in group T/DT when compared to groups S and DEX (p < 0.05). These values were significantly higher in group DEX than group T/DT. Germ cell apoptosis, eNOS and iNOS levels were significantly higher in group T/DT when compared to groups S and DEX (p < 0.05). Conclusions: DEX treatment has potential biochemical and histopathological benefits by preventing ischemia/reperfusion-related cellular damage in an experimental testicular torsion model. Preference of DEX for anesthesia during the detorsion procedure may attenuate ischemia-reperfusion injury. © 2010 S. Karger AG, Basel.

Dergi Adı Urologia Internationalis
Dergi Cilt Bilgisi 84
Dergi Sayısı 1
Sayfalar 105 - 111
Yayın Yılı 2010
Eser Adı
[dc.title]
Effect of dexmedetomidine on testicular torsion/detorsion damage in rats
Yazar
[dc.contributor.author]
Hanci V.
Yazar
[dc.contributor.author]
Erol B.
Yazar
[dc.contributor.author]
Bektaş S.
Yazar
[dc.contributor.author]
Mungan G.
Yazar
[dc.contributor.author]
Yurtlu S.
Yazar
[dc.contributor.author]
Tokgöz H.
Yazar
[dc.contributor.author]
Can M.
Yayın Yılı
[dc.date.issued]
2010
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Background and Objective: We assessed the antioxidant activity of dexmedetomidine (DEX) during an ischemic period in a rat model of testicular torsion/detorsion (T/DT) by using biochemical and histopathological methods. Methods: Wistar Albino male rats weighing 250-300 g were divided into three groups: sham (group S, n = 7); torsion/detorsion (group T/DT, n = 7), and DEX treatment (group DEX, n = 7). In the T/DT group, right testes were rotated 720° for 1 h. Group S served for normal basal values. Rats in group T/DT were operated to make T/DT, this group served as a control group. Group DEX received intraperitoneal DEX 10 µg · kg-1 after the 30-min torsion period. For measurement of total antioxidant enzyme activities and malondialdehyde (MDA) levels, testes of 7 animals in each group were excised after 4 h of reperfusion. Germ cell apoptosis was evaluated using the apoptosis protease-activating factor 1 (APAF-1) antibody in all groups and also on the expressions of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) were assessed within the bilateral testes. Results: Mean MDA levels in group T/DT were significantly higher than in groups S and DEX (p < 0.05). There were also significant decreases in mean total antioxidant activities in group T/DT when compared to groups S and DEX (p < 0.05). These values were significantly higher in group DEX than group T/DT. Germ cell apoptosis, eNOS and iNOS levels were significantly higher in group T/DT when compared to groups S and DEX (p < 0.05). Conclusions: DEX treatment has potential biochemical and histopathological benefits by preventing ischemia/reperfusion-related cellular damage in an experimental testicular torsion model. Preference of DEX for anesthesia during the detorsion procedure may attenuate ischemia-reperfusion injury. © 2010 S. Karger AG, Basel.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Dexmedetomidine
Konu Başlıkları
[dc.subject]
Ischemia/reperfusion injury
Konu Başlıkları
[dc.subject]
Surgical detorsion
Konu Başlıkları
[dc.subject]
Testicular torsion
Konu Başlıkları
[dc.subject]
Testicular torsion/detorsion, rats
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0042-1138
İlk Sayfa Sayısı
[dc.identifier.startpage]
105
Son Sayfa Sayısı
[dc.identifier.endpage]
111
Dergi Adı
[dc.relation.journal]
Urologia Internationalis
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
84
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1159/000273476
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5298
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
31
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 09:45
Google Kontrol
Tıklayınız
groups significantly levels (group testes higher antioxidant apoptosis values compared served treatment torsion nitric biochemical testicular torsion/detorsion during histopathological period synthase assessed activities Results expressions endothelial bilateral (eNOS) inducible (iNOS) within Conclusions Preference Karger injury
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms