The effects of the synovium on chondrocyte growth: An experimental study

Purpose: The objective of this study was to evaluate the effects of synovium on the proliferation of the cartilage tissue and chondrocytes using a rabbit knee model as an in vivo synovial culture medium. Methods: Twelve New Zealand rabbits were used as the animal model in this investigation. Standard size chondral and osteochondral cartilage grafts were taken from, respectively, the left and right knees of all the animals. Two groups of 6 animals were formed: in Group I (synovium group), grafts were placed into the synovial tissue and in group II (patellar tendon group) behind the patellar tendon of the corresponding knees. After 4 months, samples were collected and evaluated macroscopically by measuring their dimensions (vertical = D1, horizontal = D2, and depth = D3) and volumes, and histologically by counting the chondrocyte number using camera lucida method. Results: Macroscopically, the increase in average D1, D2, and D3 measurements and volume in the osteochondral specimens were significantly higher compared to the chondral specimens in both groups (P < 0.05). However, no significant difference was observed between the two groups in terms of macroscopic values. Histologically, the mean chondrocyte counts in osteochondral and chondral specimens for Group I (synovium) were 20.2 and 18.1, and for Group II (patellar tendon) were 18.7 and 15.6, respectively. The mean number of chondrocytes was found to be significantly higher in osteochondral specimens than that of chondral specimens in either group (P < 0.05). Overall average chondrocyte count was significantly higher for Group I compared to Group II (P < 0.05). Conclusion: Transplantation of the cartilage grafts into the synovial tissue in rabbit knees significantly enhanced the chondrocyte production compared with the group where the grafts were transplanted into intra-articular patellar tendon. The results of this study indicate that native synovial tissue may have the potential to be used as an in vivo culture medium for osteochondral tissue growth. © 2011 Springer-Verlag.

Yazar Bilge O.
Doral M.N.
Atesok K.
Atay O.A.
Donmez G.
Turhan E.
Uzumcugil A.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/8060
Tek Biçim Adres 10.1007/s00167-010-1391-y
Konu Başlıkları Cartilage regeneration
Chondrocyte
Knee
Synovium
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Knee Surgery, Sports Traumatology, Arthroscopy
Dergi Cilt Bilgisi 19
Dergi Sayısı 7
Sayfalar 1214 - 1223
Yayın Yılı 2011
Eser Adı
[dc.title]
The effects of the synovium on chondrocyte growth: An experimental study
Yazar
[dc.contributor.author]
Bilge O.
Yazar
[dc.contributor.author]
Doral M.N.
Yazar
[dc.contributor.author]
Atesok K.
Yazar
[dc.contributor.author]
Atay O.A.
Yazar
[dc.contributor.author]
Donmez G.
Yazar
[dc.contributor.author]
Turhan E.
Yazar
[dc.contributor.author]
Uzumcugil A.
Yayın Yılı
[dc.date.issued]
2011
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Purpose: The objective of this study was to evaluate the effects of synovium on the proliferation of the cartilage tissue and chondrocytes using a rabbit knee model as an in vivo synovial culture medium. Methods: Twelve New Zealand rabbits were used as the animal model in this investigation. Standard size chondral and osteochondral cartilage grafts were taken from, respectively, the left and right knees of all the animals. Two groups of 6 animals were formed: in Group I (synovium group), grafts were placed into the synovial tissue and in group II (patellar tendon group) behind the patellar tendon of the corresponding knees. After 4 months, samples were collected and evaluated macroscopically by measuring their dimensions (vertical = D1, horizontal = D2, and depth = D3) and volumes, and histologically by counting the chondrocyte number using camera lucida method. Results: Macroscopically, the increase in average D1, D2, and D3 measurements and volume in the osteochondral specimens were significantly higher compared to the chondral specimens in both groups (P < 0.05). However, no significant difference was observed between the two groups in terms of macroscopic values. Histologically, the mean chondrocyte counts in osteochondral and chondral specimens for Group I (synovium) were 20.2 and 18.1, and for Group II (patellar tendon) were 18.7 and 15.6, respectively. The mean number of chondrocytes was found to be significantly higher in osteochondral specimens than that of chondral specimens in either group (P < 0.05). Overall average chondrocyte count was significantly higher for Group I compared to Group II (P < 0.05). Conclusion: Transplantation of the cartilage grafts into the synovial tissue in rabbit knees significantly enhanced the chondrocyte production compared with the group where the grafts were transplanted into intra-articular patellar tendon. The results of this study indicate that native synovial tissue may have the potential to be used as an in vivo culture medium for osteochondral tissue growth. © 2011 Springer-Verlag.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Cartilage regeneration
Konu Başlıkları
[dc.subject]
Chondrocyte
Konu Başlıkları
[dc.subject]
Knee
Konu Başlıkları
[dc.subject]
Synovium
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0942-2056
Sponsor YAYINCI
[dc.description.sponsorship]
Acknowledgments This study was supported by funds from the Hacettepe University, the Center of Scientific Researches. We wish to thank Reha ALPAR PhD Prof. and Sevilay KARAHAN PhD for the biostatistical evaluations.
İlk Sayfa Sayısı
[dc.identifier.startpage]
1214
Son Sayfa Sayısı
[dc.identifier.endpage]
1223
Dergi Adı
[dc.relation.journal]
Knee Surgery, Sports Traumatology, Arthroscopy
Dergi Sayısı
[dc.identifier.issue]
7
Dergi Cilt Bilgisi
[dc.identifier.volume]
19
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1007/s00167-010-1391-y
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/8060
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
19
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 10:25
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Tıklayınız
specimens osteochondral tissue chondrocyte significantly chondral grafts synovial tendon groups higher compared cartilage average group) number (patellar patellar animals rabbit culture medium chondrocytes respectively potential native Springer-Verlag macroscopic between observed difference significant growth However values
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