A novel genetic lineage of Tula orthohantavirus in Altai voles (Microtus obscurus) from Turkey

Orthohantaviruses (family Hantaviridae order Bunyavirales) are emerging pathogens with a significant impact on human health. They are transmitted via aerosolized excreta of rodents which also act as reservoir hosts, constituting a unique route for dispersion. Dobrava-Belgrade and Puumala orthohantaviruses have been previously reported from Anatolia, in rodents, case reports and occasional outbreaks. We have collected rodents at several locations during a surveillance study in eastern Anatolia. The specimens were morphologically-identified and various tissues were screened via a generic orthohantavirus reverse transcription polymerase chain reaction assay. DNA barcoding via mitochondrial cytochrome b sequencing was performed in rodents with detectable orthohantavirus sequences. High throughput sequencing was performed for viral genome characterization. Fifty rodents were collected and identified morphologically as Microtus spp. (96%) and Apodemus spp. (4%). Orthohantavirus sequences were detected in lung and spleen or liver tissues of 4 voles (8%), barcoded as Microtus obscurus. The virus sequences were identified as Tula orthohantavirus (TULV) and near-complete genomic segments of the prototype viral genome, tentatively named as the Tula orthohantavirus-Turkey (TULV-T), could be characterized. Putative open reading frames for viral nucleocapsid and a nonstructural protein on the S segment, glycoproteins G1 and G2 on the M segment and viral replicase on the L segment were identified on the TULV-T. Several minor sequence variants were further characterized. No evidence of recombination could be detected and pairwise comparisons displayed over 95% amino acid sequence identities to various Eurasian TULV strains. Phylogenetic analyses revealed distinct clustering of all genome segments from previously-characterized TULV strains via various approaches and models. Here, TULV-T constituted a novel lineage, forming an intermediate among Asian and European TULV lineages. This report describes the initial documentation of TULV circulation and its potential reservoir in Anatolia. The extent of virus dispersion, alternate hosts or outcomes of human exposure require elucidation. © 2018 Elsevier B.V.

Yazar Polat C.
Ergünay K.
Irmak S.
Erdin M.
Brinkmann A.
Çetintaş O.
Çoğal M.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/4000
Tek Biçim Adres 10.1016/j.meegid.2018.11.015
Konu Başlıkları Arvicolinae
Hantavirus
Rodentia
Turkey
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Infection, Genetics and Evolution
Dergi Cilt Bilgisi 67
Sayfalar 150 - 158
Yayın Yılı 2019
Eser Adı
[dc.title]
A novel genetic lineage of Tula orthohantavirus in Altai voles (Microtus obscurus) from Turkey
Yazar
[dc.contributor.author]
Polat C.
Yazar
[dc.contributor.author]
Ergünay K.
Yazar
[dc.contributor.author]
Irmak S.
Yazar
[dc.contributor.author]
Erdin M.
Yazar
[dc.contributor.author]
Brinkmann A.
Yazar
[dc.contributor.author]
Çetintaş O.
Yazar
[dc.contributor.author]
Çoğal M.
Yayın Yılı
[dc.date.issued]
2019
Yayıncı
[dc.publisher]
Elsevier B.V.
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Orthohantaviruses (family Hantaviridae order Bunyavirales) are emerging pathogens with a significant impact on human health. They are transmitted via aerosolized excreta of rodents which also act as reservoir hosts, constituting a unique route for dispersion. Dobrava-Belgrade and Puumala orthohantaviruses have been previously reported from Anatolia, in rodents, case reports and occasional outbreaks. We have collected rodents at several locations during a surveillance study in eastern Anatolia. The specimens were morphologically-identified and various tissues were screened via a generic orthohantavirus reverse transcription polymerase chain reaction assay. DNA barcoding via mitochondrial cytochrome b sequencing was performed in rodents with detectable orthohantavirus sequences. High throughput sequencing was performed for viral genome characterization. Fifty rodents were collected and identified morphologically as Microtus spp. (96%) and Apodemus spp. (4%). Orthohantavirus sequences were detected in lung and spleen or liver tissues of 4 voles (8%), barcoded as Microtus obscurus. The virus sequences were identified as Tula orthohantavirus (TULV) and near-complete genomic segments of the prototype viral genome, tentatively named as the Tula orthohantavirus-Turkey (TULV-T), could be characterized. Putative open reading frames for viral nucleocapsid and a nonstructural protein on the S segment, glycoproteins G1 and G2 on the M segment and viral replicase on the L segment were identified on the TULV-T. Several minor sequence variants were further characterized. No evidence of recombination could be detected and pairwise comparisons displayed over 95% amino acid sequence identities to various Eurasian TULV strains. Phylogenetic analyses revealed distinct clustering of all genome segments from previously-characterized TULV strains via various approaches and models. Here, TULV-T constituted a novel lineage, forming an intermediate among Asian and European TULV lineages. This report describes the initial documentation of TULV circulation and its potential reservoir in Anatolia. The extent of virus dispersion, alternate hosts or outcomes of human exposure require elucidation. © 2018 Elsevier B.V.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Arvicolinae
Konu Başlıkları
[dc.subject]
Hantavirus
Konu Başlıkları
[dc.subject]
Rodentia
Konu Başlıkları
[dc.subject]
Turkey
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1567-1348
İlk Sayfa Sayısı
[dc.identifier.startpage]
150
Son Sayfa Sayısı
[dc.identifier.endpage]
158
Dergi Adı
[dc.relation.journal]
Infection, Genetics and Evolution
Dergi Cilt Bilgisi
[dc.identifier.volume]
67
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.meegid.2018.11.015
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4000
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
14
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
18 Şubat 2024 23:27
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Tıklayınız
rodents sequences orthohantavirus identified various Anatolia genome segment detected TULV-T characterized Microtus sequence collected dispersion sequencing segments strains performed tissues reservoir comparisons evidence recombination pairwise replicase further Putative reading frames nucleocapsid nonstructural protein glycoproteins variants
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