Dose-dependent neuroprotective effects of melatonin on experimental spinal cord injury in rats.

BACKGROUND: This report examines the dose-dependent effects of melatonin on early lipid peroxidation levels, ultrastructural changes, and neurological function in experimental spinal cord injury (SCI) by comparing them with therapeutic levels of methylprednisone in rats. METHODS: SCI was performed by an aneurysm clip placed extradurally at the level of T10. Rats were randomly divided into six groups of 10 rats each. Group 1 (sham) received only laminectomy; group 2 (control) received SCI; group 3 (placebo) received SCI and physiological saline; group 4 received methylprednisone (30 mg/kg); groups 5 and 6 received melatonin at doses of 50 or 100 mg/kg, respectively, after SCI. Rats were neurologically tested 24 hours after trauma. Spinal cord samples were harvested for both lipid peroxidation levels and ultrastructural histopathological evaluation. RESULTS: Neurological scores of rats were not different in SCI groups. Lipid peroxidation levels are significantly restricted only in methylprednisone group at 24 hours. Melatonin-treated groups showed more ultrastructural improvement on electron microscope studies when compared with methylprednisone group. However, the therapeutic effects of melatonin were mainly observed on white matter of spinal cord in ultrastructural investigation. There was significant difference between melatonin dose groups increasing with dose. CONCLUSIONS: Results showed that melatonin has no significant dose-dependent effects on early lipid peroxidation bur rather some neuroprotective effects on both axons and myelin sheaths of white matter in ultrastructural observations when compared with methylprednisone. These effects significantly augmented with dose increase.

Yazar Gül S.
Celik S.E.
Kalayci M.
Taşyürekli M.
Cokar N.
Bilge T.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/5194
Tek Biçim Adres 10.1016/j.surneu.2005.03.036
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
Dergi Adı Surgical neurology
Dergi Cilt Bilgisi 64
Dergi Sayısı 4
Sayfalar 355 - 361
Yayın Yılı 2005
Eser Adı
[dc.title]
Dose-dependent neuroprotective effects of melatonin on experimental spinal cord injury in rats.
Yazar
[dc.contributor.author]
Gül S.
Yazar
[dc.contributor.author]
Celik S.E.
Yazar
[dc.contributor.author]
Kalayci M.
Yazar
[dc.contributor.author]
Taşyürekli M.
Yazar
[dc.contributor.author]
Cokar N.
Yazar
[dc.contributor.author]
Bilge T.
Yayın Yılı
[dc.date.issued]
2005
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
BACKGROUND: This report examines the dose-dependent effects of melatonin on early lipid peroxidation levels, ultrastructural changes, and neurological function in experimental spinal cord injury (SCI) by comparing them with therapeutic levels of methylprednisone in rats. METHODS: SCI was performed by an aneurysm clip placed extradurally at the level of T10. Rats were randomly divided into six groups of 10 rats each. Group 1 (sham) received only laminectomy; group 2 (control) received SCI; group 3 (placebo) received SCI and physiological saline; group 4 received methylprednisone (30 mg/kg); groups 5 and 6 received melatonin at doses of 50 or 100 mg/kg, respectively, after SCI. Rats were neurologically tested 24 hours after trauma. Spinal cord samples were harvested for both lipid peroxidation levels and ultrastructural histopathological evaluation. RESULTS: Neurological scores of rats were not different in SCI groups. Lipid peroxidation levels are significantly restricted only in methylprednisone group at 24 hours. Melatonin-treated groups showed more ultrastructural improvement on electron microscope studies when compared with methylprednisone group. However, the therapeutic effects of melatonin were mainly observed on white matter of spinal cord in ultrastructural investigation. There was significant difference between melatonin dose groups increasing with dose. CONCLUSIONS: Results showed that melatonin has no significant dose-dependent effects on early lipid peroxidation bur rather some neuroprotective effects on both axons and myelin sheaths of white matter in ultrastructural observations when compared with methylprednisone. These effects significantly augmented with dose increase.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0090-3019
İlk Sayfa Sayısı
[dc.identifier.startpage]
355
Son Sayfa Sayısı
[dc.identifier.endpage]
361
Dergi Adı
[dc.relation.journal]
Surgical neurology
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
64
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.surneu.2005.03.036
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5194
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
7
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
07 Şubat 2024 20:09
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Tıklayınız
received ultrastructural groups methylprednisone melatonin effects levels peroxidation spinal significantly compared matter significant showed dose-dependent therapeutic microscope scores studies different RESULTS electron histopathological improvement evaluation Neurological restricted Melatonin-treated BACKGROUND However increase augmented observations sheaths myelin
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