Effect of chronic fluorosis on lipid peroxidation and histology of kidney tissues in first- and second-generation rats

This experiment was designed to investigate the lipid peroxidation and histological effects of chronic fluorosis on first- and second-generation rat kidney tissues. Sixteen virgin female Wistar rats were mated with eight males (2 : 1) for approx 12 h to obtain first-generation rats. Mating was confirmed by the presence of sperm in vaginal smears. Sperm in vaginal smears was observed in 10 of 16 rats (d 0). These rats were identified as pregnant and included in this experiment. Pregnant rats were divided into two experimental groups (control and fluoride-supplemented), each containing five rats. The pregnant rats in the fluoride-supplemented group were exposed to 30 mg/L sodium fluoride (NaF) in commercial drinking water containing 0.07 mg/L NaF throughout the gestation and the lactation periods. After the lactation period, young animals (first generation [F1]) were exposed to the same amount of NaF in drinking water for 4 mo. At the end of the 4-mo experimental period, nine randomly chosen male rats (F1) were sacrificed, and the kidneys were removed for the histological and lipid peroxidation examinations. The remaining eight female rats were mated with four males (2 :1) for approx 12 h to obtain second-generation rats. Six female were identified as pregnant, and treated similarly throughout the gestation and the lactation periods. After the lactation period, the young male rats (second-generation male rats [F2]) were also treated similarly for 4 mo. At the end of the 4-mo experimental period, nine randomly chosen male rats (F2) were sacrificed, and the kidneys were removed for the histological and lipid peroxidation examinations. The rats in the control groups underwent the same procedure without NaF supplementation. It was found that the plasma fluoride and kidney TBARS levels of fluoride-supplemented F1 and F2 rats were higher than controls. Hydropic epithelial cell degenerations and moderate tubular dilatation were observed in some proximal and distal tubules. There were markedly focal mononuclear cell infiltrations and hemorrhage at some areas of the interstitium, especially at the corticomedullar junction. Mononuclear cell infiltrations were also evident in some peritubular and perivascular areas. Most of the vascular structures were congestive. Many Bowman capsules were narrowed. The severe degenerative changes in most of the shrunken glomerules and vascular congestion were also observed. It is concluded that chronic fluorosis causes a marked destruction in kidney tissues of F1 and F2 rats by causing lipid peroxidation.

Yazar Karaoz E.
Oncu M.
Gulle K.
Kanter M.
Gultekin F.
Karaoz S.
Mumcu E.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/5285
Tek Biçim Adres 10.1385/BTER:102:1-3:199
Konu Başlıkları Fluorosis
Histology
Kidney
Lipid peroxidation
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Biological Trace Element Research
Dergi Cilt Bilgisi 102
Dergi Sayısı 01.Mar
Sayfalar 199 - 208
Yayın Yılı 2004
Eser Adı
[dc.title]
Effect of chronic fluorosis on lipid peroxidation and histology of kidney tissues in first- and second-generation rats
Yazar
[dc.contributor.author]
Karaoz E.
Yazar
[dc.contributor.author]
Oncu M.
Yazar
[dc.contributor.author]
Gulle K.
Yazar
[dc.contributor.author]
Kanter M.
Yazar
[dc.contributor.author]
Gultekin F.
Yazar
[dc.contributor.author]
Karaoz S.
Yazar
[dc.contributor.author]
Mumcu E.
Yayın Yılı
[dc.date.issued]
2004
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
This experiment was designed to investigate the lipid peroxidation and histological effects of chronic fluorosis on first- and second-generation rat kidney tissues. Sixteen virgin female Wistar rats were mated with eight males (2 : 1) for approx 12 h to obtain first-generation rats. Mating was confirmed by the presence of sperm in vaginal smears. Sperm in vaginal smears was observed in 10 of 16 rats (d 0). These rats were identified as pregnant and included in this experiment. Pregnant rats were divided into two experimental groups (control and fluoride-supplemented), each containing five rats. The pregnant rats in the fluoride-supplemented group were exposed to 30 mg/L sodium fluoride (NaF) in commercial drinking water containing 0.07 mg/L NaF throughout the gestation and the lactation periods. After the lactation period, young animals (first generation [F1]) were exposed to the same amount of NaF in drinking water for 4 mo. At the end of the 4-mo experimental period, nine randomly chosen male rats (F1) were sacrificed, and the kidneys were removed for the histological and lipid peroxidation examinations. The remaining eight female rats were mated with four males (2 :1) for approx 12 h to obtain second-generation rats. Six female were identified as pregnant, and treated similarly throughout the gestation and the lactation periods. After the lactation period, the young male rats (second-generation male rats [F2]) were also treated similarly for 4 mo. At the end of the 4-mo experimental period, nine randomly chosen male rats (F2) were sacrificed, and the kidneys were removed for the histological and lipid peroxidation examinations. The rats in the control groups underwent the same procedure without NaF supplementation. It was found that the plasma fluoride and kidney TBARS levels of fluoride-supplemented F1 and F2 rats were higher than controls. Hydropic epithelial cell degenerations and moderate tubular dilatation were observed in some proximal and distal tubules. There were markedly focal mononuclear cell infiltrations and hemorrhage at some areas of the interstitium, especially at the corticomedullar junction. Mononuclear cell infiltrations were also evident in some peritubular and perivascular areas. Most of the vascular structures were congestive. Many Bowman capsules were narrowed. The severe degenerative changes in most of the shrunken glomerules and vascular congestion were also observed. It is concluded that chronic fluorosis causes a marked destruction in kidney tissues of F1 and F2 rats by causing lipid peroxidation.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Fluorosis
Konu Başlıkları
[dc.subject]
Histology
Konu Başlıkları
[dc.subject]
Kidney
Konu Başlıkları
[dc.subject]
Lipid peroxidation
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0163-4984
İlk Sayfa Sayısı
[dc.identifier.startpage]
199
Son Sayfa Sayısı
[dc.identifier.endpage]
208
Dergi Adı
[dc.relation.journal]
Biological Trace Element Research
Dergi Sayısı
[dc.identifier.issue]
01.Mar
Dergi Cilt Bilgisi
[dc.identifier.volume]
102
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1385/BTER:102:1-3:199
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5285
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
27
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 06:34
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Tıklayınız
peroxidation period lactation female observed kidney experimental pregnant histological gestation throughout periods fluoride drinking infiltrations exposed fluoride-supplemented kidneys similarly treated examinations removed sacrificed experiment chosen randomly containing groups approx smears second-generation tissues fluorosis chronic identified
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