Effects of caffeine on oxidant-antioxidant mechanisms in the rat liver

Objective: Caffeine (1, 3, 7-trimethylxanthine) is a purine alkaloid which exists in a variety of foods and drinks. Today, caffeine is a regularly consumed substance, found in coffee, tea, chocolate and cola. The main aim of our study was to compare the potential antioxidant effects of oral caffeine intake in rat the liver at two different doses over a short period of time. Methods: We measured malondialdehyde (MDA) levels, which is a product of lipid peroxidation, in rat livers following caffeine administration. In addition, we evaluated superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione S transferase (GST) activities as well as glutathione (GSH) levels in the liver. Thirty male Wister rats were used. Rats were equally divided into three groups. Group 1 was the control group, Group 2 received 30 mg/kg of caffeine and Group 3 received 100 mg/kg caffeine (non-toxic high dose) orally for 14 days (a short time period). Results: Our results showed that the 30mg/kg and 100 mg/kg caffeine doses decreased lipid peroxidation in liver. Antioxidant enzyme activities in the rat liver, like SOD, catalase, GPx and GST, showed a statistically significant increase with caffeine intake. Liver glutathione levels, in comparison to the control group, showed a slight increase, but this was not statistically significant. Results from the Spearman analysis showed a strong negative correlation between MDA levels and GPx, GST and SOD activities. Tissue GST activity and tissue catalase activity showed a strong positive correlation. Conclusion: Decreased lipid peroxidation and increased antioxidant enzyme activities demonstrate improved control of oxidative stress, suggesting that these doses of caffeine may have antioxidant activity. ©Copyright 2012 by Gazi University Medical Faculty.

Yazar Demirtaş C.
Ofluoglu E.
Hussein A.
Paşaoglu H.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/5446
Tek Biçim Adres 10.5152/gmj.2012.04
Konu Başlıkları Antioxidant enzymes
Caffeine
Liver
Malondialdehyde
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
Dergi Adı Gazi Medical Journal
Dergi Cilt Bilgisi 23
Dergi Sayısı 1
Sayfalar 13 - 18
Yayın Yılı 2012
Eser Adı
[dc.title]
Effects of caffeine on oxidant-antioxidant mechanisms in the rat liver
Yazar
[dc.contributor.author]
Demirtaş C.
Yazar
[dc.contributor.author]
Ofluoglu E.
Yazar
[dc.contributor.author]
Hussein A.
Yazar
[dc.contributor.author]
Paşaoglu H.
Yayın Yılı
[dc.date.issued]
2012
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Objective: Caffeine (1, 3, 7-trimethylxanthine) is a purine alkaloid which exists in a variety of foods and drinks. Today, caffeine is a regularly consumed substance, found in coffee, tea, chocolate and cola. The main aim of our study was to compare the potential antioxidant effects of oral caffeine intake in rat the liver at two different doses over a short period of time. Methods: We measured malondialdehyde (MDA) levels, which is a product of lipid peroxidation, in rat livers following caffeine administration. In addition, we evaluated superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione S transferase (GST) activities as well as glutathione (GSH) levels in the liver. Thirty male Wister rats were used. Rats were equally divided into three groups. Group 1 was the control group, Group 2 received 30 mg/kg of caffeine and Group 3 received 100 mg/kg caffeine (non-toxic high dose) orally for 14 days (a short time period). Results: Our results showed that the 30mg/kg and 100 mg/kg caffeine doses decreased lipid peroxidation in liver. Antioxidant enzyme activities in the rat liver, like SOD, catalase, GPx and GST, showed a statistically significant increase with caffeine intake. Liver glutathione levels, in comparison to the control group, showed a slight increase, but this was not statistically significant. Results from the Spearman analysis showed a strong negative correlation between MDA levels and GPx, GST and SOD activities. Tissue GST activity and tissue catalase activity showed a strong positive correlation. Conclusion: Decreased lipid peroxidation and increased antioxidant enzyme activities demonstrate improved control of oxidative stress, suggesting that these doses of caffeine may have antioxidant activity. ©Copyright 2012 by Gazi University Medical Faculty.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Antioxidant enzymes
Konu Başlıkları
[dc.subject]
Caffeine
Konu Başlıkları
[dc.subject]
Liver
Konu Başlıkları
[dc.subject]
Malondialdehyde
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1300056X
İlk Sayfa Sayısı
[dc.identifier.startpage]
13
Son Sayfa Sayısı
[dc.identifier.endpage]
18
Dergi Adı
[dc.relation.journal]
Gazi Medical Journal
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
23
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.5152/gmj.2012.04
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5446
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
20
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
17 Şubat 2024 13:52
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caffeine showed glutathione levels activities control catalase antioxidant activity peroxidation strong intake enzyme increase correlation significant received statistically Results (non-toxic orally Antioxidant period) decreased 30mg/kg results Objective demonstrate Faculty Medical University ©Copyright suggesting stress oxidative
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