Effect of hemoglobin F and A2 on hemoglobin A1c determined by cation exchange high-performance liquid chromatography

The potential effect of increase in hemoglobin (Hb) A2 and HbF on glycated hemoglobin (HbA1c) measurements were investigated using a high-performance liquid chromatography (HPLC) method compared with an immunoturbimetric assay. Samples producing abnormal chromatograms during the measurement of routine HbA1c testing with HPLC were further analyzed to characterize abnormal Hb variants. Patients were divided into three groups that had only high HbF (group 1), only high HbA2 (group 2), and both high HbA2 and HbF (group 3). HbA1c values of patients were re-assayed using the immunoturbidimetric method (Advia, Siemens Healthcare, Germany). HbA1c levels were significantly higher in all groups measured by immunoassay than in HPLC. We found a positive correlation between HPLC and immunoturbidimetry in the group 2 and a slight correlation in the group 1. There was no correlation between the two methods in group 3. HbA1c measurement by HPLC method interfering with elevated HbA2 and HbF, especially HbF, should be verified by an immunoturbidimetric method. © 2019 Walter de Gruyter GmbH, Berlin.

Yazar Can M.
Güven B.
Akca A.S.D.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/5318
Tek Biçim Adres 10.1515/labmed-2019-0040
Konu Başlıkları HbA1c
HbA2
HbF
hemoglobin variant
HPLC
immunoassay
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
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Dergi Adı Journal of Laboratory Medicine
Sayfalar -
Yayın Yılı 2019
Eser Adı
[dc.title]
Effect of hemoglobin F and A2 on hemoglobin A1c determined by cation exchange high-performance liquid chromatography
Yazar
[dc.contributor.author]
Can M.
Yazar
[dc.contributor.author]
Güven B.
Yazar
[dc.contributor.author]
Akca A.S.D.
Yayın Yılı
[dc.date.issued]
2019
Yayıncı
[dc.publisher]
Walter de Gruyter GmbH
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
The potential effect of increase in hemoglobin (Hb) A2 and HbF on glycated hemoglobin (HbA1c) measurements were investigated using a high-performance liquid chromatography (HPLC) method compared with an immunoturbimetric assay. Samples producing abnormal chromatograms during the measurement of routine HbA1c testing with HPLC were further analyzed to characterize abnormal Hb variants. Patients were divided into three groups that had only high HbF (group 1), only high HbA2 (group 2), and both high HbA2 and HbF (group 3). HbA1c values of patients were re-assayed using the immunoturbidimetric method (Advia, Siemens Healthcare, Germany). HbA1c levels were significantly higher in all groups measured by immunoassay than in HPLC. We found a positive correlation between HPLC and immunoturbidimetry in the group 2 and a slight correlation in the group 1. There was no correlation between the two methods in group 3. HbA1c measurement by HPLC method interfering with elevated HbA2 and HbF, especially HbF, should be verified by an immunoturbidimetric method. © 2019 Walter de Gruyter GmbH, Berlin.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
HbA1c
Konu Başlıkları
[dc.subject]
HbA2
Konu Başlıkları
[dc.subject]
HbF
Konu Başlıkları
[dc.subject]
hemoglobin variant
Konu Başlıkları
[dc.subject]
HPLC
Konu Başlıkları
[dc.subject]
immunoassay
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
2567-9430
Dergi Adı
[dc.relation.journal]
Journal of Laboratory Medicine
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1515/labmed-2019-0040
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/5318
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
19
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
28 Şubat 2024 08:34
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method correlation (group immunoturbidimetric measurement groups between abnormal hemoglobin higher immunoassay Gruyter measured significantly Berlin levels Germany) Healthcare Siemens Walter positive methods elevated especially should interfering slight verified immunoturbidimetry (Advia re-assayed high-performance producing Samples immunoturbimetric
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