Comparison of Spermac and Diff-quik staining methods in the assessment of sperm morphology in asthenozoopermia cases [Asthenozoospermia olgularinda sperm morfolojisi degerlendirmede Spermac ve Diff-quik boya yöntemlerinin karşilaştirilmasi]

Sperm motility problems (Asthenozoopermia) is one of the major causes of male infertility. In cases with asthenozoopermia infertility increases to a greater extent when sperm morphology disorders are added to coexisting motility disorders. A total of 65 males (21 males with sperm motility disorder and 44 males without this disorder) who referred to the in vitro fertilization unit of Dokuz Eylul University Medical School between November 15, 2004 and April 5, 2005 were included in the present study. Spermac and Diff-quik staining methods were used in the assessment of sperm morphology in all semen samples. Test results showed consistency with both staining methods on 55 semen samples examined. Sperm morphology was normal in 32 semen samples with consistent test results by both methods, while the percentage of morphology was low in 23 cases. In 7 of remaining 10 cases, the sperm morphology was in the normal range with Diff-quik staining method while an abnormal percentage was detected with Spermac method. In 3 cases the sperm morphology was within normal limits with Spermac staining method while Diff-quik staining method demonstrated abnormal sperm morphology. Statistical analysis with Mc Nemar test demonstrated that both staining methods were reliable. Since neither of the two methods used in the evaluation of sperm morphology has proven superiority to the other, a cost reduction may be provided by choosing the economical one. © Gülhane Askeri Tip Akademisi 2008.

Dergi Adı Gulhane Medical Journal
Dergi Cilt Bilgisi 50
Dergi Sayısı 1
Sayfalar 23 - 26
Yayın Yılı 2008
Eser Adı
[dc.title]
Comparison of Spermac and Diff-quik staining methods in the assessment of sperm morphology in asthenozoopermia cases [Asthenozoospermia olgularinda sperm morfolojisi degerlendirmede Spermac ve Diff-quik boya yöntemlerinin karşilaştirilmasi]
Yazar
[dc.contributor.author]
Ok E.
Yazar
[dc.contributor.author]
Özyurt D.
Yazar
[dc.contributor.author]
Gülekli B.
Yayın Yılı
[dc.date.issued]
2008
Yayıncı
[dc.publisher]
Gulhane Medical School, University of Health Sciences
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Sperm motility problems (Asthenozoopermia) is one of the major causes of male infertility. In cases with asthenozoopermia infertility increases to a greater extent when sperm morphology disorders are added to coexisting motility disorders. A total of 65 males (21 males with sperm motility disorder and 44 males without this disorder) who referred to the in vitro fertilization unit of Dokuz Eylul University Medical School between November 15, 2004 and April 5, 2005 were included in the present study. Spermac and Diff-quik staining methods were used in the assessment of sperm morphology in all semen samples. Test results showed consistency with both staining methods on 55 semen samples examined. Sperm morphology was normal in 32 semen samples with consistent test results by both methods, while the percentage of morphology was low in 23 cases. In 7 of remaining 10 cases, the sperm morphology was in the normal range with Diff-quik staining method while an abnormal percentage was detected with Spermac method. In 3 cases the sperm morphology was within normal limits with Spermac staining method while Diff-quik staining method demonstrated abnormal sperm morphology. Statistical analysis with Mc Nemar test demonstrated that both staining methods were reliable. Since neither of the two methods used in the evaluation of sperm morphology has proven superiority to the other, a cost reduction may be provided by choosing the economical one. © Gülhane Askeri Tip Akademisi 2008.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
tur
Konu Başlıkları
[dc.subject]
Asthenozoopermia
Konu Başlıkları
[dc.subject]
Diff-quik
Konu Başlıkları
[dc.subject]
Sperm morphology
Konu Başlıkları
[dc.subject]
Spermac
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
1302-0471
İlk Sayfa Sayısı
[dc.identifier.startpage]
23
Son Sayfa Sayısı
[dc.identifier.endpage]
26
Dergi Adı
[dc.relation.journal]
Gulhane Medical Journal
Dergi Sayısı
[dc.identifier.issue]
1
Dergi Cilt Bilgisi
[dc.identifier.volume]
50
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4834
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
9
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
08 Şubat 2024 17:05
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Tıklayınız
morphology staining methods method normal Spermac Diff-quik motility samples results percentage abnormal demonstrated infertility disorders (Asthenozoopermia) analysis Statistical problems limits within detected remaining Akademisi Askeri Gülhane economical choosing provided reliable reduction superiority proven evaluation neither
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