A chemometric optimization of method for determination of nitrosamines in gastric juices by GC-MS

A chemometrically optimized isolation procedure combined with gas chromatography-mass spectrometry detection technique has been proposed for quantitative determination of trace levels of nitrosamines in gastric juice samples of patients with the gastrointestinal tract problems. The extraction conditions of each nitrosamine were optimized using regression modelling based on central composite design. The extraction conditions for all nitrosamines were selected to be 10.7. min for extraction time, 4.2 for pH and 23 for 2-propanol percentage in extraction solution.The obtained recoveries of nitrosamines ranged from 94.0 (NDMA) to 99.3 (NDPheA) %, and the precision of this method, as indicated by the relative standard deviations was within the range of 0.7 (NDPheA) and 2.6 (NDMA) %. The detection limits obtained from calculations by using GC-MS results based on S/N. =. 3 were found within the range from 0.3 to 1.1. pg/mL. Total nitrosamine concentrations were found at the highest concentration up to 2431.12. pg/mL in cancer patients, whereas they were found at the lowest concentration down to 12.18. pg/mL in gastritis patients. The classification results of the gastric juice samples in different patient groups were very satisfactory, allowing 100% of patients to be correctly grouped. A new mathematical model has been developed allowing for the classification of gastric juices with a 93.1% success rate based on just the ratio of MNPIZ to DNPIZ. The ratio of MNPIZ to DNPIZ might be considered as a biomarker for the classification of gastric juices of patients and might act as an indicator of increased risk for stomach cancer. © 2015 Elsevier B.V.

Yazar Akyüz M.
Ata Ş.
Dinç E.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/3886
Tek Biçim Adres 10.1016/j.jpba.2015.08.021
Konu Başlıkları Chemometric
Diseases
Gastric juices
GC-MS
Nitrosamines
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Journal of Pharmaceutical and Biomedical Analysis
Dergi Cilt Bilgisi 117
Sayfalar 26 - 36
Yayın Yılı 2016
Eser Adı
[dc.title]
A chemometric optimization of method for determination of nitrosamines in gastric juices by GC-MS
Yazar
[dc.contributor.author]
Akyüz M.
Yazar
[dc.contributor.author]
Ata Ş.
Yazar
[dc.contributor.author]
Dinç E.
Yayın Yılı
[dc.date.issued]
2016
Yayıncı
[dc.publisher]
Elsevier
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
A chemometrically optimized isolation procedure combined with gas chromatography-mass spectrometry detection technique has been proposed for quantitative determination of trace levels of nitrosamines in gastric juice samples of patients with the gastrointestinal tract problems. The extraction conditions of each nitrosamine were optimized using regression modelling based on central composite design. The extraction conditions for all nitrosamines were selected to be 10.7. min for extraction time, 4.2 for pH and 23 for 2-propanol percentage in extraction solution.The obtained recoveries of nitrosamines ranged from 94.0 (NDMA) to 99.3 (NDPheA) %, and the precision of this method, as indicated by the relative standard deviations was within the range of 0.7 (NDPheA) and 2.6 (NDMA) %. The detection limits obtained from calculations by using GC-MS results based on S/N. =. 3 were found within the range from 0.3 to 1.1. pg/mL. Total nitrosamine concentrations were found at the highest concentration up to 2431.12. pg/mL in cancer patients, whereas they were found at the lowest concentration down to 12.18. pg/mL in gastritis patients. The classification results of the gastric juice samples in different patient groups were very satisfactory, allowing 100% of patients to be correctly grouped. A new mathematical model has been developed allowing for the classification of gastric juices with a 93.1% success rate based on just the ratio of MNPIZ to DNPIZ. The ratio of MNPIZ to DNPIZ might be considered as a biomarker for the classification of gastric juices of patients and might act as an indicator of increased risk for stomach cancer. © 2015 Elsevier B.V.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Chemometric
Konu Başlıkları
[dc.subject]
Diseases
Konu Başlıkları
[dc.subject]
Gastric juices
Konu Başlıkları
[dc.subject]
GC-MS
Konu Başlıkları
[dc.subject]
Nitrosamines
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0731-7085
İlk Sayfa Sayısı
[dc.identifier.startpage]
26
Son Sayfa Sayısı
[dc.identifier.endpage]
36
Dergi Adı
[dc.relation.journal]
Journal of Pharmaceutical and Biomedical Analysis
Dergi Cilt Bilgisi
[dc.identifier.volume]
117
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.jpba.2015.08.021
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/3886
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
20
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
08 Şubat 2024 06:00
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Tıklayınız
patients extraction gastric nitrosamines classification results (NDPheA) concentration juices (NDMA) allowing within obtained cancer conditions samples detection optimized nitrosamine Elsevier lowest whereas highest stomach gastritis different correctly success developed mathematical grouped increased considered satisfactory groups
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