Combination therapy of Nigella sativa and human parathyroid hormone on bone mass, biomechanical behavior and structure in streptozotocin-induced diabetic rats

Extracts of the seeds of Nigella sativa (NS), an annual herbaceous plant of the Ranunculaceae family, have been used for many years for therapeutic purposes, including their potential anti-diabetic properties. The aim of the present study was to test the hypothesis that combined treatment with NS and human parathyroid hormone (hPTH) is more effective than treatment with NS or hPTH alone in improving bone mass, connectivity, biomechanical behaviour and strength in insulin-dependent diabetic rats. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) at a single dose of 50 mg/kg. The diabetic rats received NS (2 ml/kg/day, i.p.), hPTH (6 µg/kg/day, i.p.) or NS and hPTH combined for 4 weeks, starting 8 weeks after STZ injection. The ß-cells of the pancreatic islets of Langerhans were examined by immunohistochemical methods. In addition, bone sections of femora were processed for histomorphometry and biomechanical analysis. In diabetic rats, the ß-cells were essentially negative for insulin-immunoreactivity. NS treatment (alone or in combination with hPTH) significantly increased the area of insulin immunoreactive ß-cells in diabetic rats; however, hPTH treatment alone only led to a slightly increase in the insulin-immunoreactivity. These results suggest that NS might be used in a similar manner to insulin as a safe and effective therapy for diabetes and might be useful in the treatment of diabetic osteopenia. © 2007 Elsevier GmbH. All rights reserved.

Dergi Adı Acta Histochemica
Dergi Cilt Bilgisi 109
Dergi Sayısı 4
Sayfalar 304 - 314
Yayın Yılı 2007
Eser Adı
[dc.title]
Combination therapy of Nigella sativa and human parathyroid hormone on bone mass, biomechanical behavior and structure in streptozotocin-induced diabetic rats
Yazar
[dc.contributor.author]
Altan M.F.
Yazar
[dc.contributor.author]
Kanter M.
Yazar
[dc.contributor.author]
Donmez S.
Yazar
[dc.contributor.author]
Kartal M.E.
Yazar
[dc.contributor.author]
Buyukbas S.
Yayın Yılı
[dc.date.issued]
2007
Yayıncı
[dc.publisher]
Elsevier GmbH
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Extracts of the seeds of Nigella sativa (NS), an annual herbaceous plant of the Ranunculaceae family, have been used for many years for therapeutic purposes, including their potential anti-diabetic properties. The aim of the present study was to test the hypothesis that combined treatment with NS and human parathyroid hormone (hPTH) is more effective than treatment with NS or hPTH alone in improving bone mass, connectivity, biomechanical behaviour and strength in insulin-dependent diabetic rats. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) at a single dose of 50 mg/kg. The diabetic rats received NS (2 ml/kg/day, i.p.), hPTH (6 µg/kg/day, i.p.) or NS and hPTH combined for 4 weeks, starting 8 weeks after STZ injection. The ß-cells of the pancreatic islets of Langerhans were examined by immunohistochemical methods. In addition, bone sections of femora were processed for histomorphometry and biomechanical analysis. In diabetic rats, the ß-cells were essentially negative for insulin-immunoreactivity. NS treatment (alone or in combination with hPTH) significantly increased the area of insulin immunoreactive ß-cells in diabetic rats; however, hPTH treatment alone only led to a slightly increase in the insulin-immunoreactivity. These results suggest that NS might be used in a similar manner to insulin as a safe and effective therapy for diabetes and might be useful in the treatment of diabetic osteopenia. © 2007 Elsevier GmbH. All rights reserved.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Bone histomorphometry
Konu Başlıkları
[dc.subject]
Diabetes mellitus
Konu Başlıkları
[dc.subject]
Human parathyroid hormone
Konu Başlıkları
[dc.subject]
Nigella sativa
Konu Başlıkları
[dc.subject]
Pancreatic ß-cells
Konu Başlıkları
[dc.subject]
Rat
Konu Başlıkları
[dc.subject]
Streptozotocin
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0065-1281
İlk Sayfa Sayısı
[dc.identifier.startpage]
304
Son Sayfa Sayısı
[dc.identifier.endpage]
314
Dergi Adı
[dc.relation.journal]
Acta Histochemica
Dergi Sayısı
[dc.identifier.issue]
4
Dergi Cilt Bilgisi
[dc.identifier.volume]
109
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1016/j.acthis.2007.02.006
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4752
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
28
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 06:06
Google Kontrol
Tıklayınız
treatment diabetic ß-cells effective biomechanical insulin combined insulin-immunoreactivity injection sections essentially immunohistochemical methods negative islets pancreatic analysis histomorphometry femora processed addition examined Langerhans (alone reserved rights Elsevier osteopenia useful diabetes therapy manner combination similar suggest
6698 sayılı Kişisel Verilerin Korunması Kanunu kapsamında yükümlülüklerimiz ve çerez politikamız hakkında bilgi sahibi olmak için alttaki bağlantıyı kullanabilirsiniz.

creativecommons
Bu site altında yer alan tüm kaynaklar Creative Commons Alıntı-GayriTicari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.
Platforms