An in-vitro investigation of the effect of perfluorooctane sulphonate on cell lines of embryonic origin

Fluorinated organic compounds, such as perfluorooctane sulfonate, are stable chemicals with a wide range of industrial applications. The potential toxicity of perfluorooctane sulfonate is not well characterized, and even less known are the mechanisms underlying its toxic effects. Perfluorooctane sulfonate change of inner mitochondrial membrane permeability has been implicated as a potential mechanism of toxicity. In this study, we research that perfluorooctane sulfonate effects the expression of Apaf1 and Caspase3 genes in the amnion and fetal lung cell line that initiate the cells to undergo apoptosis. The expression of Caspase3 and Apaf1 was determined by using quantitative RT-PCR. In the study there is significant increase in expression of Caspase3 and Apaf1 in amnion and fetal lung cell line exposed to high dose (p < 0.001, p = 0.004). Also there is significant increase in cell lines exposed for a long period of time to perfluorooctane sulfonate (p = 0.001). But no significant increase was seen in the low doses and exposed for a short period of time. In conclusion, apoptotic gene expression is increase in cells exposed perfluorooctane sulfonate by dose dependent manner was determined. So this work is the first study examines the apoptotic effects of perfluorooctane sulfonate in human embryonic cells it will lead the way to the other topical studies. © 2014 Springer Science+Business Media.

Yazar Karakas-Celik S.
Aras N.
Yayın Türü Article
Tek Biçim Adres https://hdl.handle.net/20.500.12628/4247
Tek Biçim Adres 10.1007/s11033-014-3240-4
Konu Başlıkları Apaf-1
Apoptosis
Caspase3
Perfluorooctane sulphonate
Koleksiyonlar Araştırma Çıktıları | WoS | Scopus | TR-Dizin | PubMed | SOBİAD
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu
Dergi Adı Molecular Biology Reports
Dergi Cilt Bilgisi 41
Dergi Sayısı 6
Sayfalar 3755 - 3759
Yayın Yılı 2014
Eser Adı
[dc.title]
An in-vitro investigation of the effect of perfluorooctane sulphonate on cell lines of embryonic origin
Yazar
[dc.contributor.author]
Karakas-Celik S.
Yazar
[dc.contributor.author]
Aras N.
Yayın Yılı
[dc.date.issued]
2014
Yayıncı
[dc.publisher]
Kluwer Academic Publishers
Yayın Türü
[dc.type]
article
Özet
[dc.description.abstract]
Fluorinated organic compounds, such as perfluorooctane sulfonate, are stable chemicals with a wide range of industrial applications. The potential toxicity of perfluorooctane sulfonate is not well characterized, and even less known are the mechanisms underlying its toxic effects. Perfluorooctane sulfonate change of inner mitochondrial membrane permeability has been implicated as a potential mechanism of toxicity. In this study, we research that perfluorooctane sulfonate effects the expression of Apaf1 and Caspase3 genes in the amnion and fetal lung cell line that initiate the cells to undergo apoptosis. The expression of Caspase3 and Apaf1 was determined by using quantitative RT-PCR. In the study there is significant increase in expression of Caspase3 and Apaf1 in amnion and fetal lung cell line exposed to high dose (p < 0.001, p = 0.004). Also there is significant increase in cell lines exposed for a long period of time to perfluorooctane sulfonate (p = 0.001). But no significant increase was seen in the low doses and exposed for a short period of time. In conclusion, apoptotic gene expression is increase in cells exposed perfluorooctane sulfonate by dose dependent manner was determined. So this work is the first study examines the apoptotic effects of perfluorooctane sulfonate in human embryonic cells it will lead the way to the other topical studies. © 2014 Springer Science+Business Media.
Kayıt Giriş Tarihi
[dc.date.accessioned]
2019-12-23
Açık Erişim Tarihi
[dc.date.available]
2019-12-23
Yayın Dili
[dc.language.iso]
eng
Konu Başlıkları
[dc.subject]
Apaf-1
Konu Başlıkları
[dc.subject]
Apoptosis
Konu Başlıkları
[dc.subject]
Caspase3
Konu Başlıkları
[dc.subject]
Perfluorooctane sulphonate
Haklar
[dc.rights]
info:eu-repo/semantics/closedAccess
ISSN
[dc.identifier.issn]
0301-4851
İlk Sayfa Sayısı
[dc.identifier.startpage]
3755
Son Sayfa Sayısı
[dc.identifier.endpage]
3759
Dergi Adı
[dc.relation.journal]
Molecular Biology Reports
Dergi Sayısı
[dc.identifier.issue]
6
Dergi Cilt Bilgisi
[dc.identifier.volume]
41
Tek Biçim Adres
[dc.identifier.uri]
https://dx.doi.org/10.1007/s11033-014-3240-4
Tek Biçim Adres
[dc.identifier.uri]
https://hdl.handle.net/20.500.12628/4247
Görüntülenme Sayısı ( Şehir )
Görüntülenme Sayısı ( Ülke )
Görüntülenme Sayısı ( Zaman Dağılımı )
Görüntülenme
29
09.12.2022 tarihinden bu yana
İndirme
1
09.12.2022 tarihinden bu yana
Son Erişim Tarihi
09 Şubat 2024 07:07
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Tıklayınız
sulfonate perfluorooctane expression increase exposed significant Caspase3 effects apoptotic amnion period potential toxicity determined Fluorinated embryonic Science+Business Springer studies topical examines manner dependent conclusion initiate underlying mechanisms characterized applications industrial chemicals stable compounds Perfluorooctane research
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